Optical Recording of The Neuronal Activity in The Brainstem-Spinal Cord

Okada, Yasumasa; Chen, Zibin; Yoshida, Hiroyuki; Kuwana, Shun-ichi; Jiang, Wuhan; Maruiwa, Hirofumi

doi:10.1007/978-1-4615-1375-9_18

Cited by 10 publications

(3 citation statements)

References 22 publications

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“…This was due to the fact that adult rats were difficult to maintain under a viable condition in vitro because the spinal cord of adult animals is vulnerable to any lack of oxygen (Okada et al, 2001). The results of the present study should therefore be interpreted with caution, because the effects of compression may differ between juvenile and adult rats.…”

Section: Figmentioning

confidence: 86%

“…A number of optical recording techniques that permit precise observation of two-dimensional changes in the neuronal activities of the spinal cord have been reported. These include calcium imaging (Lev-Tov and O'Donovan, 1995;O'Donovan et al, 1993), intrinsic optical recording (Murase et al, 1998) and optical recording using voltage-sensitive dyes (Ikeda et al, 1998;Mochida et al, 2001;Okada et al, 2001). By using a rapidly responding voltage-sensitive dye (i.e., di-4-ANEPPS), we were able to visualize the fast processes of neural activity, previously inaccessible with slow optical recording techniques such as calcium imaging.…”

Section: Figmentioning

confidence: 99%

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Synaptic Blockade Plays a Major Role in the Neural Disturbance of Experimental Spinal Cord Compression

Yoshida

Okada²,

Maruiwa³

et al. 2003

Journal of Neurotrauma

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We analyzed dynamic processes of neural excitation propagation in the experimentally compressed spinal cord using a high-speed optical recording system. Transverse slices of the juvenile rat cervical spinal cord were stained with a voltage-sensitive dye (di-4-ANEPPS). Two components were identified in the depolarizing optical responses to dorsal root electrical stimulation: a fast component of short duration corresponding to pre-synaptic excitation and a slow component of long duration corresponding to post-synaptic excitation. In the directly compressed dorsal horn, the slow component was attenuated more (attenuated to 37.4 +/- 9.1% of the control) than the fast component (to 70.5 +/- 14.9%) (p < 0.01) at 400 msec after stimulation. Depolarizing optical responses to compression and to chemical synaptic blockade were similar. There was a regional difference between white matter (attenuated to 86.2 +/- 10.5%) and gray matter (to 72.6 +/- 10.4%) (p < 0.03) in compression-induced changes of the fast components; neural activity in the white matter was resistant to compression, especially in the dorsal root entry zone. Depolarizing optical signals in the region adjacent to the directly compressed site were also attenuated; the fast component was attenuated to 77.6 +/- 10.4% and the slow component to 31.8 +/- 11.3% of the control signals (p < 0.01). Spinal cord dysfunction induced by purely mechanical compression without tissue destruction was virtually restored with early decompression. We suggest that a disturbance of synaptic transmission plays an important role in the pathophysiological mechanisms of spinal cord compression, at least under in vitro experimental conditions of juvenile rats.

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Section: Figmentioning

confidence: 86%

Section: Figmentioning

confidence: 99%

Synaptic Blockade Plays a Major Role in the Neural Disturbance of Experimental Spinal Cord Compression

Yoshida

Okada²,

Maruiwa³

et al. 2003

Journal of Neurotrauma

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“…The voltage-sensitive dye imaging technique has been described in detail previously [ 28 ]. In brief, for staining, preparations were kept for 30 min in mock CSF containing the voltage-sensitive dye Di-4-ANEPPS (7.5 mg/mL in 0.1% DMSO, Molecular Probes, Eugene, OR, USA), before being kept for at least 30 min in normal mock CSF.…”

Section: Methodsmentioning

confidence: 99%

Effect of carbamazepine and gabapentin on excitability in the trigeminal subnucleus caudalis of neonatal rats using a voltage-sensitive dye imaging technique

et al. 2015

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BackgroundThe antiepileptic drugs carbamazepine and gabapentin are effective in treating neuropathic pain and trigeminal neuralgia. In the present study, to analyze the effects of carbamazepine and gabapentin on neuronal excitation in the spinal trigeminal subnucleus caudalis (Sp5c) in the medulla oblongata, we recorded temporal changes in nociceptive afferent activity in the Sp5c of trigeminal nerve-attached brainstem slices of neonatal rats using a voltage-sensitive dye imaging technique.ResultsElectrical stimulation of the trigeminal nerve rootlet evoked changes in the fluorescence intensity of dye in the Sp5c. The optical signals were composed of two phases, a fast component with a sharp peak followed by a long-lasting component with a period of more than 500 ms. This evoked excitation was not influenced by administration of carbamazepine (10, 100 and 1,000 μM) or gabapentin (1 and 10 μM), but was increased by administration of 100 μM gabapentin. This evoked excitation was increased further in low Mg2+ (0.8 mM) conditions, and this effect of low Mg2+ concentration was antagonized by 30 μM DL-2-amino-5-phosphonopentanoic acid (AP5), a N-methyl-d-aspartate (NMDA) receptor blocker. The increased excitation in low Mg2+ conditions was also antagonized by carbamazepine (1,000 μM) and gabapentin (100 μM).ConclusionCarbamazepine and gabapentin did not decrease electrically evoked excitation in the Sp5c in control conditions. Further excitation in low Mg2+ conditions was antagonized by the NMDA receptor blocker AP5. Carbamazepine and gabapentin had similar effects to AP5 on evoked excitation in the Sp5c in low Mg2+ conditions. Thus, we concluded that carbamazepine and gabapentin may act by blocking NMDA receptors in the Sp5c, which contributes to its anti-hypersensitivity in neuropathic pain.

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Chemosensitive Neuronal Network Organization in the Ventral Medulla Analyzed by Dynamic Voltage-Imaging

Okada¹,

Kuwana²,

Masumiya³

et al. 2008

Integration in Respiratory Control

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Optical Recording of The Neuronal Activity in The Brainstem-Spinal Cord

Cited by 10 publications

References 22 publications

Synaptic Blockade Plays a Major Role in the Neural Disturbance of Experimental Spinal Cord Compression

Synaptic Blockade Plays a Major Role in the Neural Disturbance of Experimental Spinal Cord Compression

Effect of carbamazepine and gabapentin on excitability in the trigeminal subnucleus caudalis of neonatal rats using a voltage-sensitive dye imaging technique

Chemosensitive Neuronal Network Organization in the Ventral Medulla Analyzed by Dynamic Voltage-Imaging

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