Optical properties of tissues quantified by Fourier-transform light scattering

Ding, Huafeng; Nguyen, Freddy T.; Boppart, Stephen A.; Popescu, Gabriel

doi:10.1364/ol.34.001372

Cited by 64 publications

(47 citation statements)

References 17 publications

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“…[30][31][32] It has been shown that the knowledge of the amplitude and phase associated with an optical field transmitted through tissues captures the entire information regarding light-tissue interaction, including scattering properties. [33][34][35][36] Yet, the potential of QPI for label-free pathology has not been explored. Here we employ spatial light interference microscopy (SLIM), [37][38][39] a new white light QPI method developed in our laboratory, to image the entire unstained prostate and breast biopsies and perform a side-by-side comparison with stained pathological slides.…”

Section: Introductionmentioning

confidence: 99%

Tissue refractive index as marker of disease

et al. 2011

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Abstract. The gold standard in histopathology relies on manual investigation of stained tissue biopsies. A sensitive and quantitative method for in situ tissue specimen inspection is highly desirable, as it would allow early disease diagnosis and automatic screening. Here we demonstrate that quantitative phase imaging of entire unstained biopsies has the potential to fulfill this requirement. Our data indicates that the refractive index distribution of histopathology slides, which contains information about the molecular scale organization of tissue, reveals prostate tumors and breast calcifications. These optical maps report on subtle, nanoscale morphological properties of tissues and cells that cannot be recovered by common stains, including hematoxylin and eosin. We found that cancer progression significantly alters the tissue organization, as exhibited by consistently higher refractive index variance in prostate tumors versus normal regions. Furthermore, using the quantitative phase information, we obtained the spatially resolved scattering mean free path and anisotropy factor g for entire biopsies and demonstrated their direct correlation with tumor presence. In essence, our results show that the tissue refractive index reports on the nanoscale tissue architecture and, in principle, can be used as an intrinsic marker for cancer diagnosis. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Section: Introductionmentioning

confidence: 99%

Tissue refractive index as marker of disease

et al. 2011

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“…The growing scientific interest and increased number of studies using FTLS is indicative of its effectiveness in the study of various phenomena in biophysics and cell biology. [8][9][10][11] To measure light scattering from sickle RBCs, we prepared blood samples extracted from a patient with SCD as well as from a healthy individual under a research protocol approved by the institutional review board (IRB). The SCD patient was under treatment with hydroxyurea.…”

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confidence: 99%

Anisotropic light scattering of individual sickle red blood cells

et al. 2012

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“…3. In order to analyze the behavior of anisotropy at the different wavelengths, we normalized the magnitude squared of the Fourier transform to obtain the probability density map of the angular scattering intensity [23,24] [see Figs. 4(a) and 4(d)].…”

Section: Resultsmentioning

confidence: 99%

Measurement of multispectral scattering properties in mouse brain tissue

Min

Ban

Wang

et al. 2017

Biomed. Opt. Express

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Abstract:We present the scattering properties of mouse brain using multispectral diffraction phase microscopy. Typical diffraction phase microscopy was incorporated with the broadband light source which offers the measurement of the scattering coefficient and anisotropy in the spectral range of 550-900 nm. The regional analysis was performed for both the myeloarchitecture and cytoarchitecture of the brain tissue. Our results clearly evaluate the multispectral scattering properties in the olfactory bulb and corpus callosum. The scattering coefficient measured in the corpus callosum is about four times higher than in the olfactory bulb. It also indicates that it is feasible to realize the quantitative phase microscope in near infrared region for thick brain tissue imaging.

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Optical properties of tissues quantified by Fourier-transform light scattering

Cited by 64 publications

References 17 publications

Tissue refractive index as marker of disease

Tissue refractive index as marker of disease

Anisotropic light scattering of individual sickle red blood cells

Measurement of multispectral scattering properties in mouse brain tissue

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