Optical properties of porcine skin and subcutaneous tissue following various methods of cold storage

DeLisi, Michael P.; Peterson, Amanda M.; Tijerina, Amanda J.; Macasadia, Matthew A.; Coker, Zachary; Hood, R. Lyle; Bixler, Joel N.

doi:10.1117/12.2611109

Cited by 1 publication

(2 citation statements)

References 17 publications

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“…Tissue samples were obtained from an exempt tissue sharing protocol, approved by the Institutional Animal Care and Use Committee (IACUC), that allowed post-mortem tissue sharing from subjects on other approved protocols. Porcine skin tissue samples were obtained by punching 10 mm biopsies from the back and flank regions of the deceased pigs [10]. Dermis and subcutaneous fat tissue were manually sectioned from the biopsies into samples with thicknesses ranging from 0.78 to 2.60 mm, with an average of 1.48 mm and a standard deviation of 0.29 mm.…”

Section: Tissue Preparation and Mountingmentioning

confidence: 99%

“…Recently DeLisi et al found minimal differences in the optical properties of fresh, refrigerated, and frozen in porcine dermis and epidermis using a goniometric spectrophotometry setup. Due to the high standard deviations found in these measurements, it was concluded that while there was minimal change, any significant difference due to temperature-related storage may have been lost in the uncertainty of the measurements [10].…”

Section: Introductionmentioning

confidence: 95%

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The effects of cold storage on double integrating sphere optical properties measurements of porcine skin and subcutaneous fat

Lopez

Keppler

Macasadia

et al. 2023

Optical Interactions With Tissue and Cells XXXIV

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Accurate values of the optical properties of skin and subcutaneous fat are important for a variety of applications, such as optical imaging techniques and computational modeling of possible hazardous laser exposure. Several studies are available in the published literature that report skin optical properties, but the method of tissue preparation and storage in these experiments can be variable. These methods include the application of some form of cold storage, such as refrigeration or freezing, which may in turn affect the optical properties of the tissues compared to the in vivo or freshly excised case. We measured the absorption and scattering coefficients of skin and subcutaneous fat samples prior to and following various methods of cold storage, particularly refrigeration, slow freezing, and flash freezing. Tissues were collected from two subjects in order to capture biological variability. We employed a double integrating sphere setup and the inverse adding-doubling method to determine optical properties. The results of this investigation will help contextualize existing studies on tissue optical properties and enable informed procedural design for future measurements.

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Section: Tissue Preparation and Mountingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%