Optical properties of mouse biotissues and their optical phantoms

Krainov, A D; Mokeeva, A.; Сергеева, Е. А.; Агрба, П. Д.; Kirillin, Mikhail

doi:10.1134/s0030400x13080122

Cited by 21 publications

(22 citation statements)

References 18 publications

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“…[28][29][30] Briefly, liquid (calibration) phantoms with known optical properties (μ a , μ 0 s ) were created to span a biological relevant range of μ 0 s and μ a in tissue. 31,32 The phantom optical properties, system configuration, and LUT appear in Figs Sampling depth of the DRS configuration of the multimodal probe was defined as the depth reached by 50% of the photons and calculated as reported elsewhere. 23,33 Methods are described in detail in Sampling Depth in the Supplementary Material.…”

Section: Probe Diffuse Reflectance Spectroscopy Characterization: Calmentioning

confidence: 99%

Diffuse reflectance spectroscopy to monitor murine colorectal tumor progression and therapeutic response

et al. 2020

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Diffuse reflectance spectroscopy to monitor murine colorectal tumor progression and therapeutic response," J. AbstractSignificance: Many studies in colorectal cancer (CRC) use murine ectopic tumor models to determine response to treatment. However, these models do not replicate the tumor microenvironment of CRC. Physiological information of treatment response derived via diffuse reflectance spectroscopy (DRS) from murine primary CRC tumors provide a better understanding for the development of new drugs and dosing strategies in CRC.Aim: Tumor response to chemotherapy in a primary CRC model was quantified via DRS to extract total hemoglobin content (tHb), oxygen saturation (StO 2 ), oxyhemoglobin, and deoxyhemoglobin in tissue.Approach: A multimodal DRS and imaging probe (0.78 mm outside diameter) was designed and validated to acquire diffuse spectra longitudinally-via endoscopic guidance-in developing colon tumors under 5-fluoruracil (5-FU) maximum-tolerated (MTD) and metronomic regimens. A filtering algorithm was developed to compensate for positional uncertainty in DRS measurements Results: A maximum increase in StO 2 was observed in both MTD and metronomic chemotherapy-treated murine primary CRC tumors at week 4 of neoadjuvant chemotherapy, with 21 AE 6% and 17 AE 6% fold changes, respectively. No significant changes were observed in tHb. Conclusion:Our study demonstrates the feasibility of DRS to quantify response to treatment in primary CRC models.

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Section: Probe Diffuse Reflectance Spectroscopy Characterization: Calmentioning

confidence: 99%

Diffuse reflectance spectroscopy to monitor murine colorectal tumor progression and therapeutic response

et al. 2020

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“…7 and 2 were not present with the transurethral light delivery method used to image the ex vivo prostate (Fig. 18,[30][31][32][35][36][37] In addition, the artifact appears in a similar relative orientation to seed reverberations in the ultrasound images of Figs. However, they are unlikely to be caused by an in vivo acoustic absorber, primarily because this artifact is located farther from the light source than the most distant seed in Fig.…”

Section: Echo Cluttermentioning

confidence: 99%

In vivovisualization of prostate brachytherapy seeds with photoacoustic imaging

et al. 2014

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We conducted a canine study to investigate the in vivo feasibility of photoacoustic imaging for intraoperative updates to brachytherapy treatment plans. A fiber coupled to a 1064-nm Nd:YAG laser was inserted into high-dose-rate brachytherapy needles, which diffused light spherically. These needles were inserted through the perineum into the prostate for interstitial light delivery and the resulting acoustic waves were detected with a transrectal ultrasound probe. Postoperative computed tomography images and ex vivo photoacoustic images confirmed seed locations. Limitations with insufficient light delivery were mitigated with short-lag spatial coherence (SLSC) beamforming, providing a 10-20 dB contrast improvement over delay-and-sum (DAS) beamforming for pulse energies ranging from 6.8 to 10.5 mJ with a fiber-seed distance as large as 9.5 mm. For the same distance and the same range of energy densities, signal-to-noise ratios (SNRs) were similar while the contrast-to-noise ratio (CNR) was higher in SLSC compared to DAS images. Challenges included visualization of signals associated with the interstitial fiber tip and acoustic reverberations between seeds separated by ≤ 2 mm. Results provide insights into the potential for clinical translation to humans.

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“…Optical properties were modeled at a laser wavelength of 1064 nm, using the values for canine prostates 40 and 100% India ink 41 reported in Table 2. Note that the optical absorption of India ink is similar (within an order of magnitude) to that of stainless steel brachytherapy seeds.…”

Section: Monte Carlo Simulationmentioning

confidence: 99%

Transurethral light delivery for prostate photoacoustic imaging

et al. 2015

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Abstract. Photoacoustic imaging has broad clinical potential to enhance prostate cancer detection and treatment, yet it is challenged by the lack of minimally invasive, deeply penetrating light delivery methods that provide sufficient visualization of targets (e.g., tumors, contrast agents, brachytherapy seeds). We constructed a sidefiring fiber prototype for transurethral photoacoustic imaging of prostates with a dual-array (linear and curvilinear) transrectal ultrasound probe. A method to calculate the surface area and, thereby, estimate the laser fluence at this fiber tip was derived, validated, applied to various design parameters, and used as an input to three-dimensional Monte Carlo simulations. Brachytherapy seeds implanted in phantom, ex vivo, and in vivo canine prostates at radial distances of 5 to 30 mm from the urethra were imaged with the fiber prototype transmitting 1064 nm wavelength light with 2 to 8 mJ pulse energy. Prebeamformed images were displayed in real time at a rate of 3 to 5 frames per second to guide fiber placement and beamformed offline. A conventional delay-and-sum beamformer provided decreasing seed contrast (23 to 9 dB) with increasing urethra-to-target distance, while the shortlag spatial coherence beamformer provided improved and relatively constant seed contrast (28 to 32 dB) regardless of distance, thus improving multitarget visualization in single and combined curvilinear images acquired with the fiber rotating and the probe fixed. The proposed light delivery and beamforming methods promise to improve key prostate cancer detection and treatment strategies.

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Optical properties of mouse biotissues and their optical phantoms

Cited by 21 publications

References 18 publications

Diffuse reflectance spectroscopy to monitor murine colorectal tumor progression and therapeutic response

Diffuse reflectance spectroscopy to monitor murine colorectal tumor progression and therapeutic response

In vivovisualization of prostate brachytherapy seeds with photoacoustic imaging

Transurethral light delivery for prostate photoacoustic imaging

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