Optical projection tomography for rapid whole mouse brain imaging

Nguyen, David; Marchand, Paul J.; Planchette, Arielle; Nilsson, Julia; Sison, Miguel; Extermann, Jérôme; López, Antonio; Sylwestrzak, Marcin; Sordet-Dessimoz, Jessica; Schmidt-Christensen, Anja; Holmberg, Dan; Ville, Dimitri Van De; Lasser, Theo

doi:10.1364/boe.8.005637

Cited by 30 publications

(23 citation statements)

References 45 publications

(58 reference statements)

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“…Optical projection tomography (OPT), essentially an optical equivalent of X‐ray computed tomography (CT), is a noninvasive 3‐dimensional imaging approach of biological tissues or whole body of small specimen . It fills the gap of mesoscopic range of specimen sizes biometric measurement . It has important applications in biomedical research .…”

Section: Introductionmentioning

confidence: 99%

Dual‐modality optical projection tomography reconstruction method from fewer views

Dou

Liu

Yang

2018

Journal of Biophotonics

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In optical projection tomography (OPT), for longitudinal living model studies, multiple measurements of the same sample are required at different time points. It is important to decrease both the total acquisition time and the light dose to the sample. We improved the ordered subsets expectation maximization reconstruction algorithm for OPT, which reduces the acquisition time and number of projections greatly compared with filtered back projection (FBP), and obtained satisfactory reconstructed images. Using zebrafish, in transmission and fluorescence mode, we demonstrate the capability of the method to reconstruct image from downsampled projection subsets. The result shows that the reconstruction image quality of the proposed method using 30 projections is comparable to that of FBP using 720 projections. The total acquisition procedure can be finished in a few seconds. The method also provides OPT with the remarkable capability to resist noises and artifacts. Projection image and fused image of zebrafish.

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Section: Introductionmentioning

confidence: 99%

Dual‐modality optical projection tomography reconstruction method from fewer views

Dou

Liu

Yang

2018

Journal of Biophotonics

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“…This allows the light to traverse the whole tissue section without much degradation and thus enable imaging, for example, of intact whole mouse brains. Some microscope configurations that exploit tissue clearing are optical projection tomography [122][123][124], light-sheet microscopy [125][126][127][128][129][130], clearing assisted scattering tomography [111], optical frequency domain imaging [111], and expansion super-resolution microscopy [131]. Despite the opportunity offered by tissue clearing to perform an optical imaging of complete whole brains, this process presents a few drawbacks such as a long preparation time, tissue shrinkage, protein and lipid loss, and the usage of highly toxic chemical reagents [132,133].…”

Section: Alternatives To Sbhmentioning

confidence: 99%

A Review of Intrinsic Optical Imaging Serial Blockface Histology (ICI-SBH) for Whole Rodent Brain Imaging

2019

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In recent years, multiple serial histology techniques were developed to enable whole rodent brain imaging in 3-D. The main driving forces behind the emergence of these imaging techniques were the genome-wide atlas of gene expression in the mouse brain, the pursuit of the mouse brain connectome, and the BigBrain project. These projects rely on the use of optical imaging to target neuronal structures with histological stains or fluorescent dyes that are either expressed by transgenic mice or injected at specific locations in the brain. Efforts to adapt the serial histology acquisition scheme to use intrinsic contrast imaging (ICI) were also put forward, thus leveraging the natural contrast of neuronal tissue. This review focuses on these efforts. First, the origin of optical contrast in brain tissue is discussed with emphasis on the various imaging modalities exploiting these contrast mechanisms. Serial blockface histology (SBH) systems using ICI modalities are then reported, followed by a review of some of their applications. These include validation studies and the creation of multimodal brain atlases at a micrometer resolution. The paper concludes with a perspective of future developments, calling for a consolidation of the SBH research and development efforts around the world. The goal would be to offer the neuroscience community a single standardized open-source SBH solution, including optical design, acquisition automation, reconstruction algorithms, and analysis pipelines.

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“…9 Optical imaging modalities, including fluorescence, multiphoton fluorescence, bioluminescence, optical projection tomography, optical coherence tomography (OCT), and diffuse optical tomography (DOT), were demonstrated for in vivo animal imaging. [10][11][12] These methods used nonionizing radiation, unlike CT scan (x-ray), SPET, and PET (gamma rays), and can provide structural as well as functional information at high resolution. [13][14][15][16] However, due to the strong optical absorption and scattering of tissue, the imaging depth was limited to ∼0.1 mm (roughly the mean free path) in conventional widefield optical microscopy.…”

Section: Introductionmentioning

confidence: 99%

Photoacoustic imaging in the second near-infrared window: a review

2019

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Photoacoustic (PA) imaging is an emerging medical imaging modality that combines optical excitation and ultrasound detection. Because ultrasound scatters much less than light in biological tissues, PA generates high-resolution images at centimeters depth. In recent years, wavelengths in the second near-infrared (NIR-II) window (1000 to 1700 nm) have been increasingly explored due to its potential for preclinical and clinical applications. In contrast to the conventional PA imaging in the visible (400 to 700 nm) and the first NIR-I (700 to 1000 nm) window, PA imaging in the NIR-II window offers numerous advantages, including high spatial resolution, deeper penetration depth, reduced optical absorption, and tissue scattering. Moreover, the second window allows a fivefold higher light excitation energy density compared to the visible window for enhancing the imaging depth significantly. We highlight the importance of the second window for PA imaging and discuss the various NIR-II PA imaging systems and contrast agents with strong absorption in the NIR-II spectral region. Numerous applications of NIR-II PA imaging, including whole-body animal imaging and human imaging, are also discussed.

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Optical projection tomography for rapid whole mouse brain imaging

Cited by 30 publications

References 45 publications

Dual‐modality optical projection tomography reconstruction method from fewer views

Dual‐modality optical projection tomography reconstruction method from fewer views

A Review of Intrinsic Optical Imaging Serial Blockface Histology (ICI-SBH) for Whole Rodent Brain Imaging

Photoacoustic imaging in the second near-infrared window: a review

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