Optical Photothermal Infrared Microspectroscopy with Simultaneous Raman – A New Non-Contact Failure Analysis Technique for Identification of &lt;10 μm Organic Contamination in the Hard Drive and other Electronics Industries

Kansiz, Mustafa; Prater, C. B.; Dillon, Eoghan; Lo, Michael; Anderson, Jay; Marcott, Curtis; Demissie, Abel T.; Chen, Yanling; Kunkel, Gary J.

doi:10.1017/s1551929520000917

Cited by 46 publications

(42 citation statements)

References 18 publications

(28 reference statements)

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“…The probe was a CW 532 nm visible variable power laser, the photothermal effect was detected through the modulation of the green laser intensity induced by the pulsed IR laser. Further details about the fundamentals of the technique and the instrument itself can be found in references 16 , 20 and in the Supplementary Information. Spectra were averaged for 20–50 scans with 1 s acquisition time per spectra to generate data of sufficient signal-to-noise ratio.…”

Section: Methodsmentioning

confidence: 99%

“…For this study, two aspects of O-PTIR were critical: first, the spatial resolution of O-PTIR (~300 nm) 4 , 20 , and second, measurements in non-contact mode. Submicron spatial resolution was necessary to trace protein structures inside cells; the non-contact mode was essential to preserve 100 nm thin Si 3 N 4 membrane used for S-XRF imaging.…”

Section: Introductionmentioning

confidence: 99%

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Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons

et al. 2021

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Alzheimer’s disease (AD) is the most common cause of dementia, costing about 1% of the global economy. Failures of clinical trials targeting amyloid-β protein (Aβ), a key trigger of AD, have been explained by drug inefficiency regardless of the mechanisms of amyloid neurotoxicity, which are very difficult to address by available technologies. Here, we combine two imaging modalities that stand at opposite ends of the electromagnetic spectrum, and therefore, can be used as complementary tools to assess structural and chemical information directly in a single neuron. Combining label-free super-resolution microspectroscopy for sub-cellular imaging based on novel optical photothermal infrared (O-PTIR) and synchrotron-based X-ray fluorescence (S-XRF) nano-imaging techniques, we capture elemental distribution and fibrillary forms of amyloid-β proteins in the same neurons at an unprecedented resolution. Our results reveal that in primary AD-like neurons, iron clusters co-localize with elevated amyloid β-sheet structures and oxidized lipids. Overall, our O-PTIR/S-XRF results motivate using high-resolution multimodal microspectroscopic approaches to understand the role of molecular structures and trace elements within a single neuronal cell.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons

et al. 2021

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“…The probe was a CW 532 nm visible variable power laser; the photothermal effect was detected through the modulation of the probe laser intensity induced by the pulsed IR laser. Details about the fundamentals of the technique and the instrument itself can be found in the literature [17,30,31]. To generate data with a sufficient signal-to-noise ratio, spectra were averaged from at least 10 scans with an acquisition time of 1 sec per spectra.…”

Section: Optical Photothermal Infrared Measurementsmentioning

confidence: 99%

Nano-Infrared Imaging of Primary Neurons

Freitas

Cernescu

Engdahl

et al. 2021

Cells

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Alzheimer’s disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-β (Aβ) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, Aβ proteins undergo structural changes to form β-sheet structures that are considered neurotoxic. Numerous intensive in vitro studies have provided detailed information about amyloid polymorphs; however, little is known on how amyloid β-sheet-enriched aggregates can cause neurotoxicity in relevant settings. We used scattering-type scanning near-field optical microscopy (s-SNOM) to study amyloid structures at the nanoscale, in individual neurons. Specifically, we show that in well-validated systems, s-SNOM can detect amyloid β-sheet structures with nanometer spatial resolution in individual neurons. This is a proof-of-concept study to demonstrate that s-SNOM can be used to detect Aβ-sheet structures on cell surfaces at the nanoscale. Furthermore, this study is intended to raise neurobiologists’ awareness of the potential of s-SNOM as a tool for analyzing amyloid β-sheet structures at the nanoscale in neurons without the need for immunolabeling.

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“…Characteristic IR absorbance peaks ( Figure 1d) at 1738 cm −1 (CO stretching), [21] 1470 cm −1 (-CH 2 -scissoring), [22] and 1173 cm −1 (C-O-C valence vibrations, C-O stretching) [2] along with corresponding active Raman bands ( Figure 1e) at 2876 cm −1 (-CH 2 -/-CH 3 stretching) [2] and 970 cm −1 (Si-O stretching) [23] taken at targetspecific locations near peripheral and central regions confirm the presence of PODA and the underlying native oxide layerpassivated Si-wafer substrate, which agrees well (98.1%) with Wiley's KnowItAll IR spectral database ( Figure S2, Supporting Information) and conventional Raman spectra ( Figure S3, Supporting Information) independently collected. O-PTIR+R has very recently been used to characterize depth-resolved molecules in living cells, [7] polymorphic amyloid aggregates in neurons, [24] sub-micrometer atmospheric particulates, [25] pharmaceutical dry-powder aerosols, [26] <10-µm organic contaminants on hard drives, [27] bioplastic composite interfaces, [28] high-explosive trace materials, [29] and Ruddlesden-Popper hybrid perovskite crystals exhibiting peripheral 2D/3D heterostructure formation, [30] which show excellent correlation to conventional FTIR absorbance spectra.…”

Section: Optical-photothermal Infrared (O-ptir) With Simultaneous Hypmentioning

confidence: 99%

Resolving Nanocomposite Interfaces via Simultaneous Submicrometer Optical‐Photothermal Infrared‐Raman Microspectroscopy

Wang

Dillon²,

Maharjan

et al. 2021

Adv Materials Inter

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Development and optimization of multiphase nanoscale systems and functional nanoarchitectures require a meticulous physicochemical understanding of interfacial regions and their interactions. Disordered multiphase systems like polymer nanocomposites often exhibit intricate and convoluted interfacial regions across interphases. [1-4] Resolving such nanocomposite interphases with sub-micrometer resolution and chemical exactitude is of substantial importance towards a complete quantitative understanding of nanoscale systems. [5] Vibrational spectroscopies afford the most chemical specificity, but are notably limited in spatial resolution. Conversely, electron microspectroscopies provide the highest spatial resolution but lack a high degree of chemical specificity, particularly for organic species. Electron and other high resolution microspectroscopies like scanning transmission X-ray microscopynear edge X-ray absorption fine structure spectroscopy (STXM-NEXAFS) typically require the sample to be characterized under vacuum, which can appreciably affect particle morphology and composition at the particle vacuum interface. Advancements in ubiquitous techniques like Fourier transform-infrared (FTIR) spectroscopy have culminated with the advent of the discrete-frequency quantum cascade laser (QCL), enabling single-frequency infrared (IR) imaging and faster data acquisition. [6] Notwithstanding advancements, such far-field IR vibrational microspectroscopies lack depth-resolving capabilities and are intrinsically restricted in spatial resolution by the wavelength-dependent diffraction limit of the probing IR light, which is typically between 5 and 12 µm across fingerprint regions and dependent on the particular objective lens used. [7] Efforts to circumvent such diffraction limited resolution have led to recent progress in optical-photothermal infrared (O-PTIR) microspectroscopy techniques, where selective absorbance of mid-IR excitation laser light is detected using a visible light probe laser via a photothermally induced thermal lensing effect. [8-18] In such all-optical schemes, the detectable signal is the modulated probe laser power ΔP probe , which is linearly proportional to

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Optical Photothermal Infrared Microspectroscopy with Simultaneous Raman – A New Non-Contact Failure Analysis Technique for Identification of <10 μm Organic Contamination in the Hard Drive and other Electronics Industries

Abstract: Abstract

Cited by 46 publications

References 18 publications

Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons

Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons

Nano-Infrared Imaging of Primary Neurons

Resolving Nanocomposite Interfaces via Simultaneous Submicrometer Optical‐Photothermal Infrared‐Raman Microspectroscopy

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