Optical Microscopic Techniques for Synthetic Polymer Characterization

Coceancigh, Herman; Higgins, Daniel A.; Ito, Takashi

doi:10.1021/acs.analchem.8b04694

Cited by 26 publications

(21 citation statements)

References 153 publications

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“…Applications of SPT in polymer science have been reviewed recently, and so we will only highlight a few examples here. In block copolymers, SPT has been used to assess the degree of domain alignment from the difference in diffusive motion of probes when confined within aligned versus randomly oriented domains. − Schwartz et al studied diffusion of polymers on a surface, revealing highly non-Gaussian behavior of displacements in the van Hove correlation functions. − This phenomenon was attributed to intermittent hopping along the surface, verified directly using 3D localization techniques as discussed in the above section .…”

Section: Visualizing Polymers In Motionmentioning

confidence: 99%

“…For those who have explored this field, we hope to spark discussions on the most appropriate “nails” for these “hammers” and how these techniques might evolve to become even more relevant in the future. We make no attempt to be comprehensive here, as an exhaustive review of optical microscopy in soft matter could span multiple textbooks in length, and some reviews of these topics have already been published. − Instead, we tailor our discussions exclusively to the audience of polymer scientists, discussing the unique capabilities of these methods and what sorts of problems are most appropriate.…”

mentioning

confidence: 99%

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100th Anniversary of Macromolecular Science Viewpoint: Enabling Advances in Fluorescence Microscopy Techniques

Qiang

Wang

2020

ACS Macro Lett.

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Section: Visualizing Polymers In Motionmentioning

confidence: 99%

mentioning

confidence: 99%

100th Anniversary of Macromolecular Science Viewpoint: Enabling Advances in Fluorescence Microscopy Techniques

Qiang

Wang

2020

ACS Macro Lett.

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“…Optical microscopy is probably the simplest and oldest among the microscopy techniques [88]. It is a two-dimensional imaging technique and since its time of inception, there have been incessant research upgrades to suit modern needs [89][90][91][92]. A simple optical microscope comprises of two converging lenses, an objective, and an eyepiece and utilizes the optical theory of lenses to operate, wherein light emerging from the sample will be collected by the objective and directed towards the eyepiece [93].…”

Section: Optical Microscopymentioning

confidence: 99%

Microscopic Techniques for the Analysis of Micro and Nanostructures of Biopolymers and Their Derivatives

et al. 2020

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Natural biopolymers, a class of materials extracted from renewable sources, is garnering interest due to growing concerns over environmental safety; biopolymers have the advantage of biocompatibility and biodegradability, an imperative requirement. The synthesis of nanoparticles and nanofibers from biopolymers provides a green platform relative to the conventional methods that use hazardous chemicals. However, it is challenging to characterize these nanoparticles and fibers due to the variation in size, shape, and morphology. In order to evaluate these properties, microscopic techniques such as optical microscopy, atomic force microscopy (AFM), and transmission electron microscopy (TEM) are essential. With the advent of new biopolymer systems, it is necessary to obtain insights into the fundamental structures of these systems to determine their structural, physical, and morphological properties, which play a vital role in defining their performance and applications. Microscopic techniques perform a decisive role in revealing intricate details, which assists in the appraisal of microstructure, surface morphology, chemical composition, and interfacial properties. This review highlights the significance of various microscopic techniques incorporating the literature details that help characterize biopolymers and their derivatives.

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“…Although a fluorescent image of each molecule detected by a CCD camera is a diffraction-limited spot as approximated as point spread function (PSF), the position of single molecule can be tracked at a very high accuracy, typically 10-20 nm in imaging (XY) plane by applying the localization method to the PSF [18][19][20][21][22]. The wide-field fluorescence microscopy and its related techniques have been applied to the investigation of a variety of polymer systems [23][24][25][26][27][28]. In addition, by using astigmatism imaging technique [29,30] the position of single molecule can be tracked along the optical (Z) axis at an accuracy of several tens of nanometers.…”

Section: Introductionmentioning

confidence: 99%

Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate)

Ito

Hiratsuka

Takei

et al. 2022

Photochem Photobiol Sci

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We have investigated three-dimensional distribution and diffusion behaviors of single guest dyes in 1-µm thick films of poly(2-hydroxyethyl acrylate) (PHEA) by using astigmatism imaging method. Perylene diimide derivative (BP-PDI) in the PHEA films localized along the Z-axis at ca. Z = 600-700 nm distant from the interface (Z = 0) between PHEA and glass substrate. This Z-localization was not observed in different polymer films of poly(methyl methacrylate) (PMMA), poly(methyl acrylate) (PMA), and polystyrene (PSt). Because the glass transition temperature of the PHEA is lower than the room temperature, BP-PDI in the PHEA films exhibited Brownian motion, normal diffusion on the XY plane and confined motion along the Z-direction. For elucidating the mechanism of the peculiar localization of the guest dyes along film thickness in the PHEA films, we measured diffusion behaviors of different dyes, R6G and Atto 488, in 1-µm thick PHEA films, obtaining result that the Z-distributions of the dyes were overall similar to that of BP-PDI. The result indicates that the Z-localization of the guest dyes should be ascribed not to the interaction between glass surface and guest dye but mainly to the Z-dependent property of the PHEA film. Indeed, the lateral diffusion coefficients of the guest dyes depended on their Z-positions.

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Optical Microscopic Techniques for Synthetic Polymer Characterization

Cited by 26 publications

References 153 publications

100th Anniversary of Macromolecular Science Viewpoint: Enabling Advances in Fluorescence Microscopy Techniques

100th Anniversary of Macromolecular Science Viewpoint: Enabling Advances in Fluorescence Microscopy Techniques

Microscopic Techniques for the Analysis of Micro and Nanostructures of Biopolymers and Their Derivatives

Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate)

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