Optical MEMS for displays in portable systems

Davis, Wyatt O.

doi:10.1533/9780857098610.2.569

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…Local subcellular activation with light for optogenetic experiments was achieved by a DMD ( Davis, 2013 ) with dimensions of 640 × 480, able to generate eight-bit grayscale patterns. The pattern was individually adjusted for every cell and dynamically evolved during the experiment according to the cell shape.…”

Section: Methodsmentioning

confidence: 99%

Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking

Vaidžiulytė

Macé

Battistella

et al. 2022

eLife

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Migrating cells present a variety of paths, from random to highly directional ones. While random movement can be explained by basal intrinsic activity, persistent movement requires stable polarization. Here, we quantitatively address emergence of persistent migration in RPE1 cells over long timescales. By live-cell imaging and dynamic micropatterning, we demonstrate that the Nucleus-Golgi axis aligns with direction of migration leading to efficient cell movement. We show that polarized trafficking is directed towards protrusions with a 20 min delay, and that migration becomes random after disrupting internal cell organization. Eventually, we prove that localized optogenetic Cdc42 activation orients the Nucleus-Golgi axis. Our work suggests that polarized trafficking stabilizes the protrusive activity of the cell, while protrusive activity orients this polarity axis, leading to persistent cell migration. Using a minimal physical model, we show that this feedback is sufficient to recapitulate the quantitative properties of cell migration in the timescale of hours.

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Section: Methodsmentioning

confidence: 99%

Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking

Vaidžiulytė

Macé

Battistella

et al. 2022

eLife

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show abstract

“…Local subcellular activation with light for optogenetic experiments was achieved by a Digital Micromirror Device (DMD) (Davis, 2013) with dimensions of 640 x 480, able to generate 8-bit grayscale patterns. The pattern was individually adjusted for every cell and dynamically evolved during the experiment according to the cell shape.…”

Section: Methodsmentioning

confidence: 99%

Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking

Vaidžiulytė

Macé

Battistella

et al. 2021

Preprint

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Migrating cells present a variety of paths, from non-persistent random walks to highly directional trajectories. While random movement can be easily explained by an intrinsic basal activity of the cell, persistent movement requires the cell to be stably polarized. It remains unclear how this is achieved from the regulation of underlying subcellular processes. In the context of mesenchymal migration, the ability of cells to migrate persistently over several hours require a mechanism stabilizing their protruding activity at their front. Here, we address this mechanism using human RPE1 cell line as our model. We measure, manipulate, and quantitatively perturb cell protrusive activity of the cortex as well as intracellular organization of the endomembrane trafficking system using dynamic micropatterning, pharmacological and trafficking assays, optogenetics and live-cell imaging with tracking. First, we demonstrate that the Nucleus-Golgi axis aligns with the direction of migration and its alignment with the protrusive activity leads to efficient cell movement. Then, using low doses of Nocodazole to disrupt internal cell organization, we show that long-lived polarity breaks down and migration becomes random. Next, we indicate that a flow of vesicles is directed towards the protrusive activity with a delay of 20 min. Eventually, by applying a sustained optogenetic activation, we prove that a localized Cdc42 gradient is able to orient the Nucleus-Golgi axis over a couple of hours. Taken together, our results suggest that the internal polarity axis, provided by the polarized trafficking of vesicles, is stabilizing the protrusive activity of the cell, while the protrusive activity biases this polarity axis. Using a novel minimal physical model, we show that this feedback is sufficient by itself to recapitulate the quantitative properties of cell migration in the timescale of hours. Our work highlights the importance of the coupling between high-level cellular functions in stabilizing the direction of migration over long timescales.

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Fast Neighbor Discovery in MEMS FSO Networks

Atakora

Chenji

2020

2020 International Conference on Computing, Networking and Communications (ICNC)

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Optical MEMS for displays in portable systems

Cited by 4 publications

References 26 publications

Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking

Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking

Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking

Fast Neighbor Discovery in MEMS FSO Networks

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