Optical manipulation and microfluidics for studies of single cell dynamics

Eriksson, Emma; Scrimgeour, Jan; Granéli, Annette; Ramser, Kerstin; Wellander, Rikard; Enger, Jonas; Hanstorp, Dag; Goksör, Mattias

doi:10.1088/1464-4258/9/8/s02

Cited by 52 publications

(36 citation statements)

References 38 publications

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“…It was found that there was no contamination when cells were dragged to a place with a different liquid medium and then transferred back again from different channels. This group (Eriksson et al 2007) used this technique to investigate the cell response to a sharp glucose concentration gradient between the two channels in which 25 yeast cells were moved back and forth between the two media by holographic OT. This provides a new method to investigate real-time cell responses to various extracellular environment conditions without being removed from the field of view of the microscope.…”

Section: Active Nanomanipulationmentioning

confidence: 99%

Optical tweezers for single cells

Zhang

Liu

2008

J. R. Soc. Interface.

662

441

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Optical tweezers (OT) have emerged as an essential tool for manipulating single biological cells and performing sophisticated biophysical/biomechanical characterizations. Distinct advantages of using tweezers for these characterizations include non-contact force for cell manipulation, force resolution as accurate as 100 aN and amiability to liquid medium environments. Their wide range of applications, such as transporting foreign materials into single cells, delivering cells to specific locations and sorting cells in microfluidic systems, are reviewed in this article. Recent developments of OT for nanomechanical characterization of various biological cells are discussed in terms of both their theoretical and experimental advancements. The future trends of employing OT in single cells, especially in stem cell delivery, tissue engineering and regenerative medicine, are prospected. More importantly, current limitations and future challenges of OT for these new paradigms are also highlighted in this review.

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Section: Active Nanomanipulationmentioning

confidence: 99%

Optical tweezers for single cells

Zhang

Liu

2008

J. R. Soc. Interface.

662

441

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“…Several other methods have also been coupled with microfluidics for cell immobilization and conducting controlled, complete cell assays. Flow-based active cell trapping by using control valves [14,15], non-invasive optical trapping [16][17][18], dielectrophoresis [19][20][21], surface chemistry modification techniques [22,23], arrays of physical barriers [24], cell trapping by negative pressure [25,26], and hydrodynamic methods [27][28][29] are some of these successfully established techniques.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous work, we developed an experimental platform combining a microfluidic chamber with optical tweezers and advanced time-lapse microscopy [17,30,31], which has vastly been used to identify the underlying mechanisms of different signaling pathways in Saccharomyces cerevisiae (budding yeast) cells. Cells were trapped by the optical tweezers and positioned precisely in an array format at the bottom of the microfluidic chamber.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications

et al. 2013

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Abstract:The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 μm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min −1 , moderate = 50 nL min −1 , and high = 100 nL min −1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.

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“…To this end, RT have been combined with microfluidic systems that allow fast, controlled exchange of the microliter volumes of working fluids in the sample chamber, injection and collection of the analyzed specimen through multiple inlet/outlet ports, and incorporation of various optical sorting techniques [431,432] (see Section 4.6). Such a combination of RT with a microfluidic system was employed by Ramser et al to study the oxygenation cycle in single optically trapped red blood cells [433,434]. The transition between the oxygenated and de-oxygenated hemoglobin states was triggered by flushing the working channel with the solution of sodium dithionite in HEPES buffer via electro-osmotic flow.…”

mentioning

confidence: 99%

Light at work: The use of optical forces for particle manipulation, sorting, and analysis

2008

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We review the combinations of optical micro-manipulation with other techniques and their classical and emerging applications to non-contact optical separation and sorting of micro- and nanoparticle suspensions, compositional and structural analysis of specimens, and quantification of force interactions at the microscopic scale. The review aims at inspiring researchers, especially those working outside the optical micro-manipulation field, to find new and interesting applications of these methods.

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Optical manipulation and microfluidics for studies of single cell dynamics

Cited by 52 publications

References 38 publications

Optical tweezers for single cells

Optical tweezers for single cells

Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications

Light at work: The use of optical forces for particle manipulation, sorting, and analysis

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