Optical Kerr effect induced by picosecond light pulses in tRNA solutions

Dobek, A.; Deprez, J.

doi:10.1016/0021-9797(86)90009-3

Cited by 12 publications

(2 citation statements)

References 30 publications

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“…Furthermore, the optical and the electrical anisotropy of tRNA is relatively small, when compared with DNA double helices, for example. Thus, only few reports appeared on this subject (36,37). We have been able to measure electrooptical data for various tRNA's at a particularly high accuracy by application of relatively high electrical field pulses with short rise and decay times and by evaluation of the transients with an efficient deconvolution technique.…”

Section: Discussionmentioning

confidence: 99%

Permanent dipole moment of tRNA's and variation of their structure in solution

Pörschke¹,

Antosiewicz²

1990

Biophysical Journal

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The structure of six different tRNA molecules has been analyzed in solution by electrooptical measurements and by bead model simulations. The electric dichroism measured as a function of the field strength shows that tRNA's are associated with substantial permanent dipole moments, which are in the range of 1 x 10(-27) cm(identical to 300 D; before correction for the internal directing field). Rotational diffusion time constants of tRNA molecules in their native state at 2 degrees C show a considerable variation. A particularly large value found for tRNA(Tyr) (50 ns) can be explained by its nine additional nucleotide residues. However, remarkable variations remain for tRNA molecules with the standard number of 76 nucleotide residues (tRNA(Phe) [yeast] 41.6 ns, tRNA(Val) [Escherichia coli] 44.9 ns, tRNA(Glu) [E. coli] 46.8 ns; tRNA(Phe) [E. coli] 48.3 ns). These variations indicate modulations of the tertiary structure, which may be due to a change of the L-hinge angle. Bead models are used to simulate both electric and hydrodynamic parameters of tRNA molecules according to the crystal structure of tRNA(Phe) (yeast). The asymmetric distribution of phosphate charges with respect to the center of diffusion leads, under the assumption of a constant charge reduction to 15% by ion condensation, to a theoretical dipole moment of 7.2 x 10(-28) cm, which is in reasonable agreement with the measurements. The dichroism decay curve calculated for tRNA(Phe) (yeast) is also consistent with the measurements and thus the structure in solution and in the crystal must be very similar in this case. However, our measurements also indicate that the structure of some other tRNA's in solution is different, even in cases with the same number of nucleotide residues.

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Section: Discussionmentioning

confidence: 99%

Permanent dipole moment of tRNA's and variation of their structure in solution

Pörschke¹,

Antosiewicz²

1990

Biophysical Journal

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“…This was followed by Dohek et al (1976) who studied permanent dipoie moments ana xc:) in sampies of t-RNA. More recently Dobek and Deprez (1986) have extended these studies into the picosecond time range using circularly polarized infrared picosecond pulses from a YAG laser. These experiments are exciting as they can directly probe very fast relaxing species within these nucleic acids.…”

Section: Further Commentsmentioning

confidence: 99%

Measurement of the 'optical' Kerr effect induced by nanosecond laser pulses

Tyson

Jennings

1991

J. Phys. D: Appl. Phys.

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A review is made of novel attempts to date at measuring the optical Kerr effect in liquids and liquid crystals. In the conventional Kerr effect, an applied electric field induces molecular orientation which is accompanied by birefringence in a fluid composed of anisotropic molecules. In the optical Kerr effect (OKE) the high-frequency electric field of a laser beam is the inducing mechanism. The phenomenon is thus an optically induced optical effect (an optico-optical phenomenon). It has potential as a means of measuring the optical polarizability of molecules which itself is useful for determining molecular bonding structures. In addition, the phenomenon can become the basis of optically activated switching mechanisms currently of interest in optoelectronics and optical computing. This review is a survey of the arrangements used hitherto for the measurement of this novel phenomenon using nanosecond laser pulses. The purpose of the review is to provide a basis for others who wish to measure and ultimately utilize the effect.

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Optical and hydrodynamical regimes in dilute solutions of polyelectrolytes

Drouot

Maugin

1987

Rheol Acta

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Abstract." A careful analysis of the equations governing dynamical disturbances in dilute solutions of polyelectrolytes (described on the basis of a conformational model with electromechanical interactions) allows one to place in evidence two extreme regimes, one called the optical regime, which justifies the use of optical methods for measuring the main instantaneous characteristics of the solutions, and the other that corresponds to electrohydrodynamical flows where a hierarchy can be exhibited for the various effects contributing to the flow equations.

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Optical Kerr effect induced by picosecond light pulses in tRNA solutions

Cited by 12 publications

References 30 publications

Permanent dipole moment of tRNA's and variation of their structure in solution

Permanent dipole moment of tRNA's and variation of their structure in solution

Measurement of the 'optical' Kerr effect induced by nanosecond laser pulses

Optical and hydrodynamical regimes in dilute solutions of polyelectrolytes

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