Optical Imaging in Breast Cancer Diagnosis: The Next Evolution

Herranz, Míchel; Ruibal, A

doi:10.1155/2012/863747

Cited by 72 publications

(42 citation statements)

References 49 publications

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“…Moreover, optical imaging is relatively cheap and imaging agents are easier to generate than PET tracers. As for the depth penetration issue in optical imaging, using NIR light and advanced more sensitive detection equipment, penetration depth in human tissue is now possible up to 15 cm (Herranz and Ruibal 2012). The result in Fig.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of neurotensin receptor 1 as a potential imaging target in pancreatic ductal adenocarcinoma

Yin

Wang

et al. 2017

Amino Acids

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Pancreatic cancer is one of the deadliest human malignancies and lack of effective diagnostic and therapeutic methods. Accumulating evidence suggests that the neurotensin (NT) and neurotensin receptors (NTRs) play key roles in pancreatic adenocarcinoma growth and survival. In this study, we not only evaluate the NTR1 expression in pancreatic cancer patient samples, but also explore the PET and fluorescence imaging of NTR1 expression in pancreatic cancer animal models. The NTR1 expression was evaluated by immunohistochemistry staining in clinical patient tissue samples with pancreatic ductal adenocarcinoma, insulinoma, and pancreatitis. The results showed 79.4% positive rate of NRT1 expression in pancreatic ductal adenocarcinoma, compared with 33.3% and 22.7% in insulinoma and pancreatitis samples, respectively. High NTR1 gene expression was also found in Panc-1 cells and confirmed by cell immunofluorescence. 64Cu-AmBaSar-NT and IRDye800-NT were synthesized as imaging probes and maintained the majority of NTR1 binding affinity. In vivo imaging demonstrated that 64Cu-AmBaSar-NT has prominent tumor uptake (3.76 ± 1.45 and 2.29 ± 0.10 %ID/g at 1 and 4 h post injection). NIR fluorescent imaging with IRDye800-NT demonstrated good tumor to background contrast (8.09 ± 0.38 ×108 and 6.67 ± 0.43 ×108 (p/s/cm2/sr)/(μW/cm2) at 30 and 60 min post injection). Fluorescence guided surgery was also performed as a proof of principle experiment. In summary, our results indicated that NTR1 is a promising target for pancreatic ductal adenocarcinoma imaging and therapy. The imaging probes reported here may not only be considered for improved diagnosis of pancreatic ductal adenocarcinoma, but also has the potential to be fully integrated into patient screening and treatment monitoring of future NTR1 targeted therapies.

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Section: Discussionmentioning

confidence: 99%

Evaluation of neurotensin receptor 1 as a potential imaging target in pancreatic ductal adenocarcinoma

Yin

Wang

et al. 2017

Amino Acids

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“…39 While the fusion of optical or photoacoustic imaging with other more quantitative imaging modalities can compensate for the low spatial resolution of PET or SPECT imaging, NIRF imaging remains limited by its low depth penetration, attenuation caused by overlapping tissues, and lack of quantitative methods for signal quantification. 40 …”

Section: Discussionmentioning

confidence: 99%

ImmunoPET and Near-Infrared Fluorescence Imaging of Pancreatic Cancer with a Dual-Labeled Bispecific Antibody Fragment

et al. 2017

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Dual-targeted imaging agents have shown improved targeting efficiencies in comparison to single-targeted entities. The purpose of this study was to quantitatively assess the tumor accumulation of a dual-labeled heterobifunctional imaging agent, targeting two overexpressed biomarkers in pancreatic cancer, using positron emission tomography (PET) and near-infrared fluorescence (NIRF) imaging modalities. A bispecific immunoconjugate (heterodimer) of CD105 and tissue factor (TF) Fab′ antibody fragments was developed using click chemistry. The heterodimer was dual-labeled with a radionuclide (64Cu) and fluorescent dye. PET/NIRF imaging and biodistribution studies were performed in four-to-five week old nude athymic mice bearing BxPC-3 (CD105/TF+/+) or PANC-1 (CD105/TF−/−) tumors xenografts. A blocking study was conducted to investigate the specificity of the tracer. Ex vivo tissue staining was performed to compare TF/CD105 expression in tissues with PET tracer uptake to validate in vivo results. PET imaging of 64Cu-NOTA-heterodimer-ZW800 in BxPC-3 tumor xenografts revealed enhanced tumor uptake (21.0 ± 3.4 %ID/g; n = 4) compared to the homodimer of TRC-105 (9.6 ± 2.0 %ID/g; n=4; p < 0.01) and ALT-836 (7.6 ± 3.7 %ID/g; n=4; p < 0.01) at 24 h post-injection. Blocking studies revealed that tracer uptake in BxPC-3 tumors could be decreased by four-fold with TF blocking and two-fold with CD105 blocking. In the negative model (PANC-1), heterodimer uptake was significantly lower than that found in the BxPC-3 model (3.5 ± 1.1 %ID/g; n=4; p < 0.01). The specificity was confirmed by the successful blocking of CD105 or TF, which demonstrated the dual targeting with 64Cu-NOTA-heterodimer-ZW800 provided an improvement in overall tumor accumulation. Also, fluorescence imaging validated the PET imaging, allowing for clear delineation of the xenograft tumors. Dual-labeled heterodimeric imaging agents, like 64Cu-NOTA-heterodimer-ZW800, may increase the overall tumor accumulation in comparison to single-targeted homodimers, leading to improved imaging of cancer and other related diseases.

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“…The errors in Dl a at the depths of 5 and 7 mm were 3 and 20% of the true Dl a , respectively. When a real cancer lesion with high concentrations of hemoglobin or contrast agents had l a larger than the background normal tissues as reported in the literature [9][10][11][12][13][14][15], the error may not disturb the diagnosis. The literature showed that the l a of cancer tissue was 2 -4 times as large as the l a of normal tissue.…”

Section: Although Some Mismatches In Cmentioning

confidence: 95%

“…The absorption coefficient of the photon absorber can reflect the conditions of biological tissues. Angiogenesis in cancer tissues increases the concentration of hemoglobin, which increases the absorption coefficient [9][10][11][12][13][14][15]. Some contrast agent molecules and nanoparticles increasing the light absorption coefficient are useful for finding diseased tissues [16,17] and mapping the sentinel lymph nodes [18,19].…”

Section: Introductionmentioning

confidence: 99%

Effects of the approximations of light propagation on quantitative photoacoustic tomography using two-dimensional photon diffusion equation and linearization

Okawa

Hirasawa

Kubota

et al. 2017

Opt Rev

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Quantitative photoacoustic tomography (QPAT) employing a light propagation model will play an important role in medical diagnoses by quantifying the concentration of hemoglobin or a contrast agent. However, QPAT by the light propagation model with the three-dimensional (3D) radiative transfer equation (RTE) requires a huge computational load in the iterative forward calculations involved in the updating process to reconstruct the absorption coefficient. The approximations of the light propagation improve the efficiency of the image reconstruction for the QPAT. In this study, we compared the 3D/ two-dimensional (2D) photon diffusion equation (PDE) approximating 3D RTE with the Monte Carlo simulation based on 3D RTE. Then, the errors in a 2D PDE-based linearized image reconstruction caused by the approximations were quantitatively demonstrated and discussed in the numerical simulations. It was clearly observed that the approximations affected the reconstructed absorption coefficient. The 2D PDE-based linearized algorithm succeeded in the image reconstruction of the region with a large absorption coefficient in the 3D phantom. The value reconstructed in the phantom experiment agreed with that in the numerical simulation, so that it was validated that the numerical simulation of the image reconstruction predicted the relationship between the true absorption coefficient of the target in the 3D medium and the reconstructed value with the 2D PDE-based linearized algorithm. Moreover, the the true absorption coefficient in 3D medium was estimated from the 2D reconstructed image on the basis of the prediction by the numerical simulation. The estimation was successful in the phantom experiment, although some limitations were revealed.

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Optical Imaging in Breast Cancer Diagnosis: The Next Evolution

Cited by 72 publications

References 49 publications

Evaluation of neurotensin receptor 1 as a potential imaging target in pancreatic ductal adenocarcinoma

Evaluation of neurotensin receptor 1 as a potential imaging target in pancreatic ductal adenocarcinoma

ImmunoPET and Near-Infrared Fluorescence Imaging of Pancreatic Cancer with a Dual-Labeled Bispecific Antibody Fragment

Effects of the approximations of light propagation on quantitative photoacoustic tomography using two-dimensional photon diffusion equation and linearization

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