Optical imaging for brain tissue characterization using relative fluorescence lifetime imaging

Papour, Asael; Taylor, Zachary D.; Sherman, Alexander E.; Sanchez, Daniel R.; Lucey, Gregory M.; Liau, Linda M.; Stafsudd, Oscar M.; Yong, William H.; Grundfest, Warren S.

doi:10.1117/1.jbo.18.6.060504

Cited by 10 publications

(8 citation statements)

References 10 publications

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“…The optical properties of PET compare favorably with glass coverslips when evaluated by 2P or FLIM imaging. The latter open up FLIM intra-vital brain imaging of autofluorescence using PET windows [39][40][41] . For example the intrinsically fluorescent metabolites nicotinamide adenine dinucleotide hydrogen (NADH) and flavin adenine dinucleotide (FAD) are widely used in vivo to record label-free cellular activity based on their oxidation state [42][43][44][45] .…”

Section: Discussionmentioning

confidence: 99%

Cortex-wide neural interfacing via transparent polymer skulls

Ghanbari

Carter

Rynes

et al. 2018

Preprint

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Neural computations occurring simultaneously in multiple cerebral cortical regions are critical for mediating cognition, perception and sensorimotor behaviors. Enormous progress has been made in understanding how neural activity in specific cortical regions contributes to behavior. However, there is a lack of tools that allow simultaneous monitoring and perturbing neural activity from multiple cortical regions. To fill this need, we have engineered "See-Shells" -digitally designed, morphologically realistic, transparent polymer skulls that allow long-term (>200 days) optical access to 45 mm 2 of the dorsal cerebral cortex in the mouse. We demonstrate the ability to perform mesoscopic imaging, as well as cellular and subcellular resolution two-photon imaging of neural structures up to 600 µm through the See-Shells. See-Shells implanted on transgenic mice expressing genetically encoded calcium (Ca 2+ ) indicators allow tracking of neural activities from multiple, non-contiguous regions spread across millimeters of the cortex. Further, neural probes can access the brain through perforated See-Shells, either for perturbing or recording neural activity from localized brain regions simultaneously with whole cortex imaging. As See-Shells can be constructed using readily available desktop fabrication tools and modified to fit a range of skull geometries, they provide a powerful tool for investigating brain structure and function.

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Section: Discussionmentioning

confidence: 99%

Cortex-wide neural interfacing via transparent polymer skulls

Ghanbari

Carter

Rynes

et al. 2018

Preprint

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“…The contrast image can be acquired and processed in less than a second. 5,13,14 In this letter, we demonstrate the system's capability in imaging full-field human fundus with low-excitation fluence and show structural variations in vivo.…”

Section: Imaging Autofluorescence Temporal Signatures Of the Human Ocmentioning

confidence: 92%

“…Fluorescence lifetime imaging microscopy is a robust imaging modality that has been proven to be sensitive in the detection of chemicals and molecular species in the cluttered environments. [1][2][3] The examples specific to biomedical imaging are the detection of cancerous tumors 4,5 and metabolic changes in the human fundus, aimed to characterize and provide better disease prognosis. Research efforts in the field reported various fluorescence biomarkers and structural contrast mechanisms to investigate detection capabilities for age-related macular degeneration (AMD), diabetic retinopathy, etc.…”

Section: Imaging Autofluorescence Temporal Signatures Of the Human Ocmentioning

confidence: 99%

Imaging autofluorescence temporal signatures of the human ocular fundus in vivo

et al. 2015

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We demonstrate real-time in vivo fundus imaging capabilities of our fluorescence lifetime imaging technology for the first time. This implementation of lifetime imaging uses light emitting diodes to capture full-field images capable of showing direct tissue contrast without executing curve fitting or lifetime calculations. Preliminary results of fundus images are presented, investigating autofluorescence imaging potential of various retina biomarkers for early detection of macular diseases.

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“…Furthermore, the absence of dye or injected contrast simplifies use of the technique in an intraoperative environment. Prior work have provided a conceptual approach for the DOCI algorithm; [9][10][11] and remarkable contrast have been demonstrated between different tissue types in ex vivo pilot studies with human tissue. 12,13 The aim of this work is to mathematically model the DOCI system through rigorous proofs to describe the contrast mechanism of the system and differentiate DOCI from existing fluorescence lifetime imaging methodologies.…”

Section: Introductionmentioning

confidence: 99%

Dynamic optical contrast imaging (DOCI): system theory for rapid, wide-field, multispectral optical imaging using fluorescence lifetime contrast mechanism

Cheng

Xie

Pellionisz

et al. 2019

Medical Imaging 2019: Image-Guided Procedures, Robotic Interventions, and Modeling

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Dynamic optical contrast imaging (DOCI): system theory for rapid, wide-field, multispectral optical imaging using fluorescence lifetime contrast mechanism," ABSTRACT Dynamic Optical Contrast Imaging (DOCI) is an imaging technique that generates image contrast through ratiometric measurements of the autofluorescence decay rates of aggregate fluorophores in tissue. This method enables better tissue characterization by utilizing wide-field signal integration, eliminating constraints of uniform illumination, and reducing time-intensive computations that are bottlenecks in the clinical translation of traditional fluorescence lifetime imaging. Previous works have demonstrated remarkable tissue contrast between tissue types in clinical human pilot studies. However, there are still challenges in the development of several subsystems, which results in existing works to use relative models. A comprehensive mathematical framework is presented to describe the contrast mechanism of the DOCI system to allow intraoperative quantitative imaging, which merits consideration for evaluation in measuring tissue characteristics in several important clinical settings.

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Optical imaging for brain tissue characterization using relative fluorescence lifetime imaging

Cited by 10 publications

References 10 publications

Cortex-wide neural interfacing via transparent polymer skulls

Cortex-wide neural interfacing via transparent polymer skulls

Imaging autofluorescence temporal signatures of the human ocular fundus in vivo

Dynamic optical contrast imaging (DOCI): system theory for rapid, wide-field, multispectral optical imaging using fluorescence lifetime contrast mechanism

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