Optical fine-needle aspiration biopsy in a rat model

Abstract

Fine needle aspiration biopsy technique and following histological examination show its effectiveness and safety but its performing takes several time. However, the problem of real-time analysis of pathological changes in tissues remains relevant. We demonstrate optical fine-needle biopsy method, combining a fine needle (17.5G) and a fiber-optic probe (1 mm diameter) for minimally invasive interrogation of tissue in vivo. During rat tumor experiment, we collected spectrally-resolved diffuse reflectance and fluorescence. Quantified differences between tumor and normal tissues were demonstrated and approved with morphological analysis. The proposed methodology seems promising for developing new diagnostic criteria for clinical practice.

Optical fine-needle biopsy approach for intraoperative multimodal diagnostics in minimally invasive abdominal surgery

“…Previously, a test study of the capabilities of the developed device on pathologically altered rat tissues was conducted 12 .…”

Brain metabolism changes in cases of impaired breathing or blood circulation in rodents evaluated by real time optical spectroscopy methods

“…The diffuse reflectance spectra channel included HL-2000-FHSA Tungsten Halogen Light Source ("Ocean Optics", USA) with a wavelength range of 360-2400 nm. The light collected from the tissue was filtered by 400 nm and 495 nm optical filters to attenuate backscattered radiation and then it was analyzed by a CCD Flame Spectrometer ("Ocean Optics", USA) in the range of 350-1000 nm 11 . To avoid photobleaching effect, fluorescence spectra were recorded 1-2 time per minute with the excitation laser turned off between the measurements.…”

Optical fine needle biopsy in hepatocellular carcinoma mouse model

“…The measurements were carried out using custom developed optical biopsy setup ( Fig. 1) implementing FS and DRS channels 14,15 . The LED with a wavelength of 365 nm and a laser diode with a wavelength of 450 nm were used to excite NADH and FAD autofluorescence.…”