Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-system

Hermanto, Dhony

doi:10.48129/kjs.v49i1.9400

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…The film functioned as a support material for immobilizing AgNP and AChE. Single films were made by mixing alginate hydrosol and chitosan hydrosol, as done by Hermanto et al [19]. In a petri dish, 1 μL of Tris-HCl buffer solution (pH 6.5) was added, and the mixture was stirred with a magnetic stirrer until homogeneous.…”

Section: Fabrication Of Visual Biosensormentioning

confidence: 99%

Inhibitive determination of organophosphate pesticides using acetylcholinesterase and silver nanoparticle as colorimetric probe

Hermanto,

Ismillayli,

Hamdiani

et al. 2023

Environmental Engineering Research

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Accurate and real-time detection of pesticides is needed to ensure food safety and ecosystem protection. A biosensor was developed to determine organophosphate pesticides using acetylcholinesterase (AChE) and silver nanoparticle (AgNP) immobilized on the alginate-chitosan film matrix. The sensing principle is based on organophosphate pesticides (OP) inhibiting AChE activity. AChE hydrolyzes acetylthiocoline (ATCh) to thiocholine (TCh) and acetic acid (AA). The -SH group on TCh induces AgNP aggregation, resulting in fading of the film's brownish-yellow color. The presence of OP in the sample inhibited AChE activity, leading to a decrease in TCh and color fading. The biosensor response is determined by measuring the Red Green Blue (RGB) value of the film. The biosensor offers rapid detection directly in-situ and real time without the use of sophisticated instruments, with optimal measurement conditions of pH 7, incubation time of 1 minute, and concentrations of AgNP, ATCh, and tris-HCl buffer of 10 μg/mL, 50, and 7 mM, respectively. Biosensors have excellent sensitivity, reproducibility, and selectivity. Measurement of OP levels in nine vegetable samples revealed that the proposed colorimetric probe's results agreed well with the GC result as the reference method. Therefore, this method can be applied in monitoring food and environment OP levels.

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Section: Fabrication Of Visual Biosensormentioning

confidence: 99%

Inhibitive determination of organophosphate pesticides using acetylcholinesterase and silver nanoparticle as colorimetric probe

Hermanto,

Ismillayli,

Hamdiani

et al. 2023

Environmental Engineering Research

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“…Parameter of pH is determining aspect in the uricase-PTOL film optimizing which is changes in pH could affect sensor response sensitively. Therefore, the influence of matrix and other electrocatalytic interference can be minimized by using a buffer solution , Hermanto et al, 2022. In phosphate buffer solution containing 0.6 mM UA, the pH effect on the uricase-PTOL film response was measured at intervals of 4.0 to 9.0 (Table 1).…”

Section: Applications Of Ptol Filmmentioning

confidence: 99%

Conducting Matrices of the Poly(-O-Toluidine, PTOL) Film for Immobilizing Uricase

Hermanto

Ismillayli

Zuryati

et al. 2022

molekul

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Polyaniline, PANI as conducting polymer has been used as enzyme immobilization matrix in biosensors due to its excellent stability and electrochromic effects, conductivity, and redox reversibility. However, at pH above 4, the electron transfer ability and redox activity decrease, henceit is necessary to modify the PANI structure to increase the PANI pH range. In this study, the PANI derivative, the poly(-o-toluidine, PTOL) film was produced for the uricase immobilization matrix. This study aimed to develop a supporting material that maintains catalytic activity uricase with a facile and inexpensive procedure. The PTOL was prepared by using the chemical oxidative polymerization technique with ammonium persulfate (dissolved in 1.0 M HCl) as an oxidizing agent. The FTIR spectra confirmed the success of PTOL formation by the appearance of its characteristic peaks. The UV-Vis spectra indicated that PTOL was in an emeraldine state with a semi-crystalline structure that was shown by its XRD pattern. While, SEM analysis described that the PTOL film was uniform with some granular aggregation in its surface morphology, but there is no holes or porosity were found. The use of PTOL film as a uricase matrix could maintain the uricase activity against the substrate (uric acid, UA) with excellent analytical characteristics. Therefore, PTOL film could be used as conducting matrices for immobilizing enzymes (e.g. biosensors).

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“…Working electrodes used PVC membrane and wire of Ag-AgCl while internal reference electrode (comparative electrode) utilized Ag-AgCl. Both electrodes were used in the measurement of the electromotive force (emf) (Hermanto, Mudasir, et al, 2022).…”

Section: Emf Measurementmentioning

confidence: 99%

Design and Synthesis of the Poly Vinyl Chloride (PVC) Membrane for Fe(III) Ion Selective Electrode (ISE) Based on Ester Modified Humic Acid (EHA) as an Ionophore

Linga

Siswanta

Mudasir

et al. 2022

JART

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Design and synthesis of the poly vinyl chloride (PVC) membrane as Fe(III) ion selective electrode (ISE) sensor based on EHA ionophore as an excellent sensory molecule for monitoring Fe(III) in the sample solution had been done. Simple and efficient EHA was made by condensation of carboxylic acid HA with ethanol and sulfuric acid as catalist. PVC membrane was prepared by mixed all component such as PVC powder, EHA, plasticizer (o-NPOE and DOP) and anion lipophilic (Sodium-TPB and oleic acid) with a total weight of 100 mg and dissolved in 1 mL of dry THF. The electrode membrane responses showed that the electrodes were highly selective to ion Fe3+ at pH 3 with response time of ±50 seconds. The dynamic range of the membrane ISE sensor was 10-1–10-7 M with the value of Nernst factor was ±19 mV per decade. The membrane electrode can be used for at least 5 weeks. The analytical results of Fe(III) measurement showed good confirmity with reference method (AAS), suggesting that the developed analytical tool could be used to measure and monitor Fe(III) in the real water samples.

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Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-system

Cited by 6 publications

References 24 publications

Inhibitive determination of organophosphate pesticides using acetylcholinesterase and silver nanoparticle as colorimetric probe

Inhibitive determination of organophosphate pesticides using acetylcholinesterase and silver nanoparticle as colorimetric probe

Conducting Matrices of the Poly(-O-Toluidine, PTOL) Film for Immobilizing Uricase

Design and Synthesis of the Poly Vinyl Chloride (PVC) Membrane for Fe(III) Ion Selective Electrode (ISE) Based on Ester Modified Humic Acid (EHA) as an Ionophore

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