Optical-digital restoration of out-of-focus color images detected in microscopy

Ortiz-Sosa, R.; Berriel‐Valdos, L. R.; Aguilar, J. F.; Carranza-Gallardo, Jazmin; Orlando-Guerrero, I. J.; Mejía-Romero, Sergio

doi:10.1117/12.903416

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2017

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“…Since, the concept of point source is a mathematical abstraction, very useful for developing theoretical results, but the experimental realizations it is necessary to use a real point source. We propose to use an object with enough small dimensions that the OS cannot resolve, this kind of object is commonly known as a quasi-point source (qps) [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Comparison of an optical-digital restoration technique with digital methods for microscopy defocused images

Ortiz-Sosa¹,

Berriel‐Valdos²,

Aguilar³

2017

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An optical-digital technique for restoring defocused images, and based on the quasi-point source concept, is introduced and compared with two conventional digital methods. The comparison is performed on the restoration of defocused microscopy images obtained with a conventional microscope but with three different numerical apertures. The effects of these techniques on the aberrated images are shown. We discuss and conclude about the advantages of our proposed technique.

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Section: Introductionmentioning

confidence: 99%