Optical diffraction tomography and image reconstruction to measure host cell alterations caused by divergent Plasmodium species

Ong, Jessica Jie Ying; Oh, Jeong-hun; Ang, Xiang Yong; Naidu, Renugah; Chu, Trang T. T.; Im, Jae Hyoung; Manzoor, Umar; Nguyen, Tuyet Kha; Na, Seok-Won; Han, Eun‐Taek; Davis, Christeen; Park, Won Sun; Chun, Wanjoo; Jun, Hojong; Lee, Se Jin; Na, Sunghun; Chan, Jerry Kok Yen; Park, YongKeun; Russell, Bruce; Chandramohanadas, Rajesh; Han, Jin‐Hee

doi:10.1016/j.saa.2022.122026

Cited by 7 publications

(2 citation statements)

References 63 publications

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“…Such accelerated growth may play a pivotal role in the development of CM. Nevertheless, these findings require functional analysis of the molecular mechanisms underlying the invasion of reticulocytes or erythroid precursors by the parasite and contradict in vitro studies performed on laboratory strains (Ong et al, 2023 ). This deeper understanding could pave the way for the development of molecules aimed at preventing the invasion of these cells by Plasmodium falciparum to limit CM development.…”

Section: Discussionmentioning

confidence: 99%

Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria

Fraering,

Salnot,

Gautier

et al. 2024

EMBO Mol Med

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Cerebral malaria (CM), the most lethal complication of Plasmodium falciparum severe malaria (SM), remains fatal for 15–25% of affected children despite the availability of treatment. P. falciparum infects and multiplies in erythrocytes, contributing to anemia, parasite sequestration, and inflammation. An unbiased proteomic assessment of infected erythrocytes and plasma samples from 24 Beninese children was performed to study the complex mechanisms underlying CM. A significant down-regulation of proteins from the ubiquitin–proteasome pathway and an up-regulation of the erythroid precursor marker transferrin receptor protein 1 (TFRC) were associated with infected erythrocytes from CM patients. At the plasma level, the samples clustered according to clinical presentation. Significantly, increased levels of the 20S proteasome components were associated with SM. Targeted quantification assays confirmed these findings on a larger cohort (n = 340). These findings suggest that parasites causing CM preferentially infect reticulocytes or erythroblasts and alter their maturation. Importantly, the host plasma proteome serves as a specific signature of SM and presents a remarkable opportunity for developing innovative diagnostic and prognostic biomarkers.

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Section: Discussionmentioning

confidence: 99%

Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria

Fraering,

Salnot,

Gautier

et al. 2024

EMBO Mol Med

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“…Specifically, QPI combined with waveguide trapping has been used to study alterations in the RBC morphology during planar trapping and transportation while simultaneously recording the changes in the time-lapsed images of trapped RBCs via interference microscopy to construct optical phased maps, while the hydrodynamic deformation of RBCs in a microfluidic channel via QPI has been conducted using the digital holography [25,27,[29][30][31][32]. 3D Holographic Tomography being one of the most powerful 3D QPI methods advances further by combining various techniques into multimodal operations, integrating Raman imaging, Brillouin spectroscopy or fluorescence [33][34][35].…”

Section: Microscopic Rbc Flow Analysismentioning

confidence: 99%

Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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The utilizations of microfluidic chips for single RBC (red blood cell) studies have attracted great interests in recent years to filter, trap, analyze, and release single erythrocytes for various applications. Researchers in this field have highlighted the vast potential in developing micro devices for industrial and academia usages, including lab-on-a-chip and organ-on-a-chip systems. This article critically reviews the current state-of-the-art and recent advances of microfluidics for single RBC analyses, including integrated sensors and microfluidic platforms for microscopic/tomographic/spectroscopic single RBC analyses, trapping arrays (including bifurcating channels), dielectrophoretic and agglutination/aggregation studies, as well as clinical implications covering cancer, sepsis, prenatal, and Sickle Cell diseases. Microfluidics based RBC microarrays, sorting/counting and trapping techniques (including acoustic, dielectrophoretic, hydrodynamic, magnetic, and optical techniques) are also reviewed. Lastly, organs on chips, multi-organ chips, and drug discovery involving single RBC are described. The limitations and drawbacks of each technology are addressed and future prospects are discussed.

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Illumination-Coded Optical Diffraction Tomography

Zheng,

Xie,

et al. 2024

Coded Optical Imaging

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Optical diffraction tomography and image reconstruction to measure host cell alterations caused by divergent Plasmodium species

Cited by 7 publications

References 63 publications

Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria

Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria

Advances in Microfluidics for Single Red Blood Cell Analysis

Illumination-Coded Optical Diffraction Tomography

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