Optical diagnostics of biological tissue cells during their cultivation in polymers

Letuta, S. N.; Maryakhina, Valeriya S.; Рахматуллин, Р Р

doi:10.1070/qe2011v041n04abeh014601

Cited by 11 publications

(12 citation statements)

References 7 publications

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“…Because of it, additionally the long-term fluorescence was measured from nutrient medium colored by erythrosine. As we described earlier [8], the kinetics has hyperbolic type and does not depend on pressure above samples. Consequently, it defines by annihilation of near-located of triplet molecules of dye only.…”

Section: The Experimental Resultsmentioning

confidence: 79%

“…The air pressure (oxygen concentration) above the surface of coloured biopolymer changed from 0.3 to 1.0 atm. Description of the device in detail is in [8].…”

Section: The Object and Methods Of Experimental Investigationmentioning

confidence: 99%

“…Normal and cancer mammalian cells were obtained by enzyme treatment of respective tissues cells of female mice (line BYRB) with spontaneous malignant tumors during 40 minutes at 37 o C. After that the received cell suspension was placed on the nutrient medium (biomaterial "Hyamatrix" [8]) and was incubated during several days at 37 o C. The fragmants of biomaterial with cultivated cells were coloured by erythrosine water solution with the concentration 10 -4 M. This dye was selected due to its high quantum yield in triplet state, intensive phosphorescence, high dissolving degree in water and penetration possibility into cells [9].…”

Section: The Object and Methods Of Experimental Investigationmentioning

confidence: 99%

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Long-term fluorescence of erythrosine in alive mammalian cells

Maryakhina

2016

JBPE

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Abstract. Investigation of triplet states of probes molecules located in cells and tissues is used for research of pathological processes included cancer development due to high sensitivity of triplet states lifetime to surround medium. In the work the long-term fluorescence of erythrosine located in alive mammalian cells has been analyzed by mathematical simulation. The model considers all basic processes of relaxation of probe triplet states in biological media. It is obtained that signal intensity and its form depends on oxygen concentration and viscosity of cells cytoplasm in which the dye diffuses. Interaction of erythrosine with the variety of extinguishers (included immobilization process into peptides molecules) does not impact on signal of the delayed fluorescence. Their concentration impact on cell viscosity mean only. The most important extinguisher is molecular oxygen impacting on signal. The correlation between theoretical and experimental results let to consider that the model is adequate.

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Section: The Experimental Resultsmentioning

confidence: 79%

“…The air pressure (oxygen concentration) above the surface of coloured biopolymer changed from 0.3 to 1.0 atm. Description of the device in detail is in [8].…”

Section: The Object and Methods Of Experimental Investigationmentioning

confidence: 99%

Section: The Object and Methods Of Experimental Investigationmentioning

confidence: 99%

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Long-term fluorescence of erythrosine in alive mammalian cells

Maryakhina

2016

JBPE

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“…Integral equation (3) forms the main principle of data processing in the method of photon correlation spectroscopy. The results of our experiments were processed using the DYNALS package of programs, in which approximate solution (3).…”

Section: The Results and Discussionmentioning

confidence: 99%

“…Phosphorescence kinetics was measured on experimental setup for biomedical investigations. Its description had been published in detail in our earlier work [3]. The wavelength of excitation was at 532 nm (the second harmonics of Nd:YAG laser).…”

Section: Phosphorescence Kineticsmentioning

confidence: 99%

Change of Optical Properties of Hair Cells During Malignant Tumor Development

Maryakhina

Scheglova

Anenkova

2015

JBPE

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Abstract. The results of experimental investigations on the differences of wool microstructure and its biophysical properties of healthy mice and mice with malignant tumour are represented in the paper. It is shown that destruction of wool structure as well as biochemical composition change of wool cells is during pathology development. Difference is seen even when tumour is small. We have shown that the general regularity of spectral measurements for mammary and hair cells is conserved at health state and pathology present. Taking into account this, the represented data about wool structure can be used to improve the optical techniques for non--invasive diagnostics of cancer diseases at different stages of pathology. © 2015 Samara State Aerospace University (SSAU).

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Singlet oxygen-sensitized delayed fluorescence of common water-soluble photosensitizers

Scholz

Dědic

Breitenbach

et al. 2013

Photochem Photobiol Sci

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Six common water-soluble singlet oxygen ((1)O2) photosensitizers - 5,10,15,20-tetrakis(1-methyl-4-pyridinio) porphine (TMPyP), meso-tetrakis(4-sulfonathophenyl)porphine (TPPS4), Al(III) phthalocyanine chloride tetrasulfonic acid (AlPcS4), eosin Y, rose bengal, and methylene blue - were investigated in terms of their ability to produce delayed fluorescence (DF) in solutions at room temperature. All the photosensitizers dissolved in air-saturated phosphate buffered saline (PBS, pH 7.4) exhibit easily detectable DF, which can be nearly completely quenched by 10 mM NaN3, a specific (1)O2 quencher. The DF kinetics has a biexponential rise-decay character in a microsecond time domain. Therefore, we propose that singlet oxygen-sensitized delayed fluorescence (SOSDF), where the triplet state of a photosensitizer reacts with (1)O2 giving rise to an excited singlet state of the photosensitizer, is the prevailing mechanism. It was confirmed by additional evidence, such as a monoexponential decay of triplet-triplet transient absorption kinetics, dependence of SOSDF kinetics on oxygen concentration, absence of SOSDF in a nitrogen-saturated sample, or the effect of isotopic exchange H2O-D2O. Eosin Y and AlPcS4 show the largest SOSDF quantum yield among the selected photosensitizers, whereas rose bengal possesses the highest ratio of SOSDF intensity to prompt fluorescence intensity. The rate constant for the reaction of triplet state with (1)O2 giving rise to the excited singlet state of photosensitizer was estimated to be ~/>1 × 10(9) M(-1) s(-1). SOSDF kinetics contains information about both triplet and (1)O2 lifetimes and concentrations, which makes it a very useful alternative tool for monitoring photosensitizing and (1)O2 quenching processes, allowing its detection in the visible spectral region, utilizing the photosensitizer itself as a (1)O2 probe. Under our experimental conditions, SOSDF was up to three orders of magnitude more intense than the infrared (1)O2 phosphorescence and by far the most important pathway of DF. SOSDF was also detected in a suspension of 3T3 mouse fibroblast cells, which underlines the importance of SOSDF and its relevance for biological systems.

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Optical diagnostics of biological tissue cells during their cultivation in polymers

Cited by 11 publications

References 7 publications

Long-term fluorescence of erythrosine in alive mammalian cells

Long-term fluorescence of erythrosine in alive mammalian cells

Change of Optical Properties of Hair Cells During Malignant Tumor Development

Singlet oxygen-sensitized delayed fluorescence of common water-soluble photosensitizers

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