Optical Detection of Ion-Channel-Induced Proton Transport in Supported Phospholipid Bilayers

Yang, Tsung-Han Calvin; Yee, Chanel K.; Amweg, Meri L.; Singh, Seema; Kendall, Eric L.; Dattelbaum, Andrew M.; Shreve, Andrew P.; Brinker, C. Jeffrey; Parikh, Atul N.

doi:10.1021/nl071184j

Cited by 22 publications

(20 citation statements)

References 33 publications

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“…Water interfaces with nanostructured materials have attracted much attention in recent years. [1][2][3][4][5][6][7][8][9] Interesting phenomena such as preferential ion adsorption in pores, 1,2 unique single-molecule diffusion patterns, 3 gate-controlled ion transport, 4,5 high-sensitivity ion sensing, [6][7][8] and enhanced streaming current in nanochannels 9 have been reported. The case of the water/porous-silica interface is particularly interesting.…”

Section: Introductionmentioning

confidence: 99%

Nanoporous silica-water interfaces studied by sum-frequency vibrational spectroscopy

Zhang

Singh

Tian

et al. 2009

The Journal of Chemical Physics

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Using sum-frequency vibrational spectroscopy, we found that water structure at nanoporous silica/water interfaces depended on the nanoporous film structure. For a periodic, self-assembled nanoporous film with monosized 2 nm pores occupying 20% of the top surface area, the surface vibrational spectrum was dominated by water in contact with silica, bare or covered by silane, at the top surface. It resembled the spectral characteristic of the hydrophilic water/silica or the hydrophobic water/silane interface. For a fractal nanoporous film with pores ranging from 5 to 50 nm in size occupying 90% of the top surface, the spectrum for a trimethyl silane-coated superhydrophobic porous film resembled largely that of a water/air interface. Only when the silane was completely removed would the spectrum revert to that characteristic of a hydrophilic water/silica interface. The surface charging behaviors of the bare nanoporous films in water with different pH were monitored by spectroscopic measurements and atomic force microscopy force measurements. The point of zero charge for the periodic porous film is around pH 2, similar to that of the flat silica surface. The point of zero charge could only be determined to be pH<6 for the fractal porous film because the thin fractal solid network limited the amount of surface charge and therefore, the accuracy of the measurements.

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Section: Introductionmentioning

confidence: 99%

Nanoporous silica-water interfaces studied by sum-frequency vibrational spectroscopy

Zhang

Singh

Tian

et al. 2009

The Journal of Chemical Physics

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“…While mesoporous silica particles have been widely studied and characterized for in vitro biocompatibility, high cargo capacity, tunable pore diameters and surface chemistries [31, 45–50], this report is the first in vivo demonstration of protocells as non-toxic and functionally capable non-viral spinal gene therapy vectors. The therapeutic application of protocells is highly beneficial for several critical reasons.…”

Section: Discussionmentioning

confidence: 99%

Mesoporous silica-supported lipid bilayers (protocells) for DNA cargo delivery to the spinal cord

Dengler

Liu

Kerwin

et al. 2013

Journal of Controlled Release

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Amorphous mesoporous silica nanoparticles (‘protocells’) that support surface lipid bilayers recently characterized in vitro as carrier constructs for small drug and DNA delivery are reported here as highly biocompatible both in vitro and in vivo, involving the brain and spinal cord following spinal delivery into the lumbosacral subarachnoid space (intrathecal; i.t.). Specifically, positively charged, 1, 2-Dioleoyl-3-Trimethylammonium-Propane (DOTAP) -cholesterol (DOTAP:Chol) liposome-formulated protocells revealed stable in vitro cargo release kinetics and cellular interleukin-10 (IL-10) transgene transfection. Recent approaches using synthetic non-viral vector platforms to deliver the pain-suppressive therapeutic transgene, IL-10, to the spinal subarachnoid space has yielded promising results in animal models of peripheral neuropathy, a condition involving aberrant neuronal communication within sensory pathways in the nervous system. Non-viral drug and gene delivery protocell platforms offer potential flexibility because cargo release-rates can be pH-dependent. We report here that i.t. delivery of protocells, with modified chemistry supporting a surface coating of DOTAP:Chol liposomes and containing the IL-10 transgene, results in functional suppression of pain-related behavior in rats for extended periods. This study is the first demonstration that protocell vectors offer amenable and enduring in vivo biological characteristics that can be applied to spinal gene delivery.

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“…The same functionalisation approach was used to fabricate photopatterned pH sensors. 43 These sensors integrated selective ion-channel transport polypeptides (gramicidin A) embedded in a supported phospholipid bilayer with pH-responsive mesostructured and mesoporous silica-based arrays (Fig. 19).…”

Section: Direct Luminescence Intensity Detection Of Ph With Functiona...mentioning

confidence: 99%

Optical chemical sensors based on hybrid organic–inorganic sol–gel nanoreactors

et al. 2011

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Sol-gel porous materials with tailored or nanostructured cavities have been increasingly used as nanoreactors for the enhancement of reactions between entrapped chemical reactants. The domains of applications issued from these designs and engineering are extremely wide. This tutorial review will focus on one of these domains, in particular on optical chemical sensors, which are the subject of extensive research and development in environment, industry and health.

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Optical Detection of Ion-Channel-Induced Proton Transport in Supported Phospholipid Bilayers

Cited by 22 publications

References 33 publications

Nanoporous silica-water interfaces studied by sum-frequency vibrational spectroscopy

Nanoporous silica-water interfaces studied by sum-frequency vibrational spectroscopy

Mesoporous silica-supported lipid bilayers (protocells) for DNA cargo delivery to the spinal cord

Optical chemical sensors based on hybrid organic–inorganic sol–gel nanoreactors

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