Optical Deformability of Soft Biological Dielectrics

Guck, Jochen; Ananthakrishnan, Revathi; Moon, T.; Cunningham, C. Casey; Käs, Josef A.

doi:10.1103/physrevlett.84.5451

Cited by 288 publications

(239 citation statements)

References 16 publications

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“…This concept was used to deform and measure the stress profiles of erythrocytes, which led to the development of the first optical stretcher device reported in 2000. 66 The setup was then demonstrated to stretch BALB 3T3 fibroblasts and measure their viscoelastic properties. 67 A typical optical stretcher system consists of a microchannel for loading cells into the testing region and two laser fibers located on the sides of the passageway (see Fig.…”

Section: Optical Stretchermentioning

confidence: 99%

Recent advances in microfluidic techniques for single-cell biophysical characterization

et al. 2013

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Section: Optical Stretchermentioning

confidence: 99%

Recent advances in microfluidic techniques for single-cell biophysical characterization

et al. 2013

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“…Several of the techniques for investigating cellular mechanics [12][13][14] make use of optical trapping techniques. For example, Guck and coworkers showed that single cells can be stretched by strong optical forces [15,16] …”

Section: Introductionmentioning

confidence: 99%

Long‐distance laser propulsion and deformation‐ monitoring of cells in optofluidic photonic crystal fiber

Unterkofler

Garbos

Euser

et al. 2012

Journal of Biophotonics

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We introduce a unique method for laser‐propelling individual cells over distances of 10s of cm through stationary liquid in a microfluidic channel. This is achieved by using liquid‐filled hollow‐core photonic crystal fiber (HC‐PCF). HC‐PCF provides low‐loss light guidance in a well‐defined single mode, resulting in highly uniform optical trapping and propulsive forces in the core which at the same time acts as a microfluidic channel. Cells are trapped laterally at the center of the core, typically several microns away from the glass interface, which eliminates adherence effects and external perturbations. During propagation, the velocity of the cells is conveniently monitored using a non‐imaging Doppler velocimetry technique. Dynamic changes in velocity at constant optical powers up to 350 mW indicate stress‐induced changes in the shape of the cells, which is confirmed by bright‐field microscopy. Our results suggest that HC‐PCF will be useful as a new tool for the study of single‐cell biomechanics. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…Compared to optical dynamometry using a beam-trapped bead as a probe [51], these experiments show that the main interests in using radiation pressure are the strong localization of excitation as well as its contactless character. In the meantime, Käs' group patented a new tool, called "Optical Stretcher", to probe the elasticity of red blood cells using the radiation pressure [52]. Due to its sensitivity, one of the ultimate goals of this technique is to differentiate healthy from malignancy cells from the difference in elastic response [53,54].…”

Section: Mechanical Aspectsmentioning

confidence: 99%

Laser microfluidics: fluid actuation by light

Delville¹,

Vincent²,

Schroll³

et al. 2009

J. Opt. A: Pure Appl. Opt.

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The development of microfluidic devices is still hindered by the lack of robust fundamental building blocks that constitute any fluidic system. An attractive approach is optical actuation because light field interaction is contactless and dynamically reconfigurable, and solutions have been anticipated through the use of optical forces to manipulate microparticles in flows. Following the concept of "optical chip" advanced from the optical actuation of suspensions, we propose in this survey new routes to extend this concept to microfluidic two-phase flows. First, we investigate the destabilization of fluid interfaces by the optical radiation pressure and the formation of liquid jets. We analyze the droplet shedding from the jet tip and the continuous transport in laser-sustained liquid channels. In a second part, we investigate a dissipative light-flow interaction mechanism consisting in heating locally two immiscible fluids to produce thermocapillary stresses along their interface. This opto-capillary coupling is implemented in adequate microchannel geometries to manipulate two-phase flows and propose a contactless optical toolbox including valves, droplet sorters and switches, droplet dividers or droplet mergers. Finally, we discuss radiation pressure and opto-capillary effects in the context of the "optical-chip" where flows, channels and operating functions would all be performed optically on the same device.

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Optical Deformability of Soft Biological Dielectrics

Cited by 288 publications

References 16 publications

Recent advances in microfluidic techniques for single-cell biophysical characterization

Recent advances in microfluidic techniques for single-cell biophysical characterization

Long‐distance laser propulsion and deformation‐ monitoring of cells in optofluidic photonic crystal fiber

Laser microfluidics: fluid actuation by light

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