Optical control of protein activity and gene expression by photoactivation of caged cyclofen

Hamouri, Fatima; Zhang, Weiting; Aujard, Isabelle; Saux, Thomas Le; Ducos, Bertrand; Vriz, Sophie; Jullien, Ludovic; Bensimon, David

doi:10.1016/bs.mie.2019.04.009

Cited by 3 publications

(3 citation statements)

References 55 publications

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“…[1] In the recent past, light‐controlled gene expression has been established in form of two concepts either by employing light‐sensitive proteins or light‐activatable (bio)molecules. [2] Mostly, genetically encoded light‐sensitive photoreceptors, which have their natural origin in plants or fungi (e. g. LOV domains, phytochromes or other photosensory proteins), are used to construct recombinant control elements applicable for activating or repressing transcription. [ 1b , 3 ] In contrast, light‐activatable molecules consist of a bioactive component and a photoremovable protecting group, retaining it in an inactive state until irradiation with a certain wavelength restores its bioactivity by photochemically initiated covalent bond cleavage.…”

Section: Introductionmentioning

confidence: 99%

Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems

et al. 2021

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Photocaged compounds are applied for implementing precise, optochemical control of gene expression in bacteria. To broaden the scope of UV-light-responsive inducer molecules, six photocaged carbohydrates were synthesized and photochemically characterized, with the absorption exhibiting a red-shift. Their differing linkage through ether, carbonate, and carbamate bonds revealed that carbonate and carbamate bonds are convenient. Subsequently, those compounds were successfully applied in vivo for controlling gene expression in E. coli via blue light illumination. Furthermore, benzoate-based expression systems were subjected to light control by establishing a novel photocaged salicylic acid derivative. Besides its synthesis and in vitro characterization, we demonstrate the challenging choice of a suitable promoter system for light-controlled gene expression in E. coli. We illustrate various bottlenecks during both photocaged inducer synthesis and in vivo application and possibilities to overcome them. These findings pave the way towards novel caged inducer-dependent systems for wavelength-selective gene expression.This article is part of a Special Collection dedicated to the Biotrans 2021 conference. Please see our homepage for more articles in the collection.

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Section: Introductionmentioning

confidence: 99%

Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems

et al. 2021

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“…Recently, versatile optogenetic approaches have been developed to control gene expression and protein activity in a live animal at single cell level and with temporal resolution of a few seconds [ 129 , 176 ]. Among them, an original and fast optogenetic approach is based on a conditional ERT/caged Cyclofen-OH (cCYC) induction system in vivo, allowing the activation of specific genes either permanently (by using Cre-ERT/loxP system) or transiently (by using a Gal4-ERT/UAS system) [ 125 , 126 , 127 , 128 , 129 , 130 ]. Such an optogenetic approach allows precise spatiotemporal control of gene expression and protein activity at single cell level or in few cells.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in vsDPGs expression and activity may be responsible for the phenotypic variation of NCCs derivatives via differential cell/tissue proliferation, migration, timing of differentiation and, ultimately, tissue growth and shape [ 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 ]. New tools allowing precise spatiotemporal modulation of gene activity at cellular resolution may allow to tackle these questions [ 125 , 126 , 127 , 128 , 129 , 130 ].…”

Section: Reprogramming Capacity Of Developmental Potential Guardians ...mentioning

confidence: 99%

Vertebrate Cell Differentiation, Evolution, and Diseases: The Vertebrate-Specific Developmental Potential Guardians VENTX/NANOG and POU5/OCT4 Enter the Stage

Ducos

Bensimon

Scerbo

2022

Cells

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During vertebrate development, embryonic cells pass through a continuum of transitory pluripotent states that precede multi-lineage commitment and morphogenesis. Such states are referred to as “refractory/naïve” and “competent/formative” pluripotency. The molecular mechanisms maintaining refractory pluripotency or driving the transition to competent pluripotency, as well as the cues regulating multi-lineage commitment, are evolutionarily conserved. Vertebrate-specific “Developmental Potential Guardians” (vsDPGs; i.e., VENTX/NANOG, POU5/OCT4), together with MEK1 (MAP2K1), coordinate the pluripotency continuum, competence for multi-lineage commitment and morphogenesis in vivo. During neurulation, vsDPGs empower ectodermal cells of the neuro-epithelial border (NEB) with multipotency and ectomesenchyme potential through an “endogenous reprogramming” process, giving rise to the neural crest cells (NCCs). Furthermore, vsDPGs are expressed in undifferentiated-bipotent neuro-mesodermal progenitor cells (NMPs), which participate in posterior axis elongation and growth. Finally, vsDPGs are involved in carcinogenesis, whereby they confer selective advantage to cancer stem cells (CSCs) and therapeutic resistance. Intriguingly, the heterogenous distribution of vsDPGs in these cell types impact on cellular potential and features. Here, we summarize the findings about the role of vsDPGs during vertebrate development and their selective advantage in evolution. Our aim to present a holistic view regarding vsDPGs as facilitators of both cell plasticity/adaptability and morphological innovation/variation. Moreover, vsDPGs may also be at the heart of carcinogenesis by allowing malignant cells to escape from physiological constraints and surveillance mechanisms.

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Monitoring of uncaging processes by designing photolytical reactions

Nakad

Chaud

Morville

et al. 2020

Photochem Photobiol Sci

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Optical control of protein activity and gene expression by photoactivation of caged cyclofen

Cited by 3 publications

References 55 publications

Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems

Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems

Vertebrate Cell Differentiation, Evolution, and Diseases: The Vertebrate-Specific Developmental Potential Guardians VENTX/NANOG and POU5/OCT4 Enter the Stage

Monitoring of uncaging processes by designing photolytical reactions

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