Optical coherence tomography through a rigid borescope applied to quantification of articular cartilage thickness in a porcine knee model

Jelly, Evan T.; Steelman, Zachary A.; Wax, Adam

doi:10.1364/ol.44.005590

Cited by 5 publications

(7 citation statements)

References 19 publications

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“…We have previously adapted this OCT engine for endoscopic use by adapting a commercial 30 cm rigid borescope with a 4 mm diameter. 40 In this study, a prototype 30-deg-angled rigid borescope (Foreal Spectrum) treated with an antireflective coating near 840 nm was employed. The distal profile of the borescope was 4 mm in diameter with a 20-cm long probe.…”

Section: Methodsmentioning

confidence: 99%

“…The first iteration of a borescope OCT device featured a 30-cm long flat-angled borescope. 40 However, many endoscopic applications require a low-angle direction of view (DOV) to adequately compensate for unique approach angles between the surgical entry port and operative site. For this study, a 30-deg DOV borescope was used to realize our design’s clinical potential further.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously reported the endoscopic delivery of OCT using a handheld rigid borescope 40 and a custom, portable spectral domain OCT (SD-OCT) system shown to offer comparable imaging performance to commercially available systems. 41 , 42 The potential utility of this device was first validated as a method to measure the thickness of knee cartilage for cases of severe osteoarthritis.…”

Section: Introductionmentioning

confidence: 99%

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Optical coherence tomography of small intestine allograft biopsies using a handheld surgical probe

et al. 2021

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. Significance: The current gold standard for monitoring small intestinal transplant (IT) rejection is endoscopic visual assessment and biopsy of suspicious lesions; however, these lesions are only superficially visualized by endoscopy. Invasive biopsies provide a coarse sampling of tissue health without depicting the true presence and extent of any pathology. Optical coherence tomography (OCT) presents a potential alternative approach with significant advantages over traditional white-light endoscopy. Aim: The aim of our investigation was to evaluate OCT performance in distinguishing clinically relevant morphological features associated with IT graft failure. Approach: OCT was applied to evaluate the small bowel tissues of two rhesus macaques that had undergone IT of the ileum. The traditional assessment from routine histological observation was compared with OCT captured using a handheld surgical probe during the days post-transplant and subsequently was compared with histophaology. Results: The reported OCT system was capable of identifying major biological landmarks in healthy intestinal tissue. Following IT, one nonhuman primate (NHP) model suffered a severe graft ischemia, and the second NHP graft failed due to acute cellular rejection. OCT images show visual evidence of correspondence with histological signs of IT rejection. Conclusions: Results suggest that OCT imaging has significant potential to reveal morphological changes associated with IT rejection and to improve patient outcomes overall.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optical coherence tomography of small intestine allograft biopsies using a handheld surgical probe

et al. 2021

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“…The form factor of a probe is advantageous because only the probe needs to be sterilized. Probes can be incorporated with hand-held devices to address imaging needs during minimally invasive medical procedures such as endoscopy, tumor ablation, and intravascular imaging [30,57,58] as well as in-line biomanufacturing processes where aseptic direct interrogation of the sample is needed.…”

Section: Glossarymentioning

confidence: 99%

Nondestructive testing of native and tissue-engineered medical products: adding numbers to pictures

Castro

Babakhanova

et al. 2022

Trends in Biotechnology

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Traditional destructive tests are used for quality assurance and control within manufacturing workflows. Their applicability to biomanufacturing is limited due to inherent constraints of the biomanufacturing process. To address this, photo-and acoustic-based nondestructive testing has risen in prominence to interrogate not only structure and function, but also to integrate quantitative measurements of biochemical composition to cross-correlate structural, compositional, and functional variances. We survey relevant literature related to single-mode and multimodal nondestructive testing of soft tissues, which adds numbers (quantitative measurements) to pictures (qualitative data). Native and tissue-engineered articular cartilage is highlighted because active biomanufacturing processes are being developed. Included are recent efforts and prominent trends focused on technologies for clinical and in-process biomanufacturing applications. Nondestructive testing of soft tissuesNondestructive (see Glossary) qualitative and quantitative assessment of native and tissueengineered soft tissues is a research area with great clinical and industrial potential. Specific to the biomanufacturing of tissue-engineered medical products (TEMPs), a core challenge of the field is in understanding and assessing the critical quality attributes of engineered tissues and correlating the critical process parameters and material attributes that greatly affect the intended clinical function [1]. Therefore, there is a growing need to develop nondestructive and noninvasive quantitative tools and methods to verify and to validate the critical biochemical components of TEMPs produced from a biological source, monitor extracellular matrix (ECM) development and composition, and correlate these critical components to tissue function (continuously and at predefined timepoints) [1].For example, when developing TEMPs for articular cartilage (AC), the mechanical properties (function) of native tissue are used to qualify the robustness and project the long-term efficacy of developing and clinically used products [2]. One method of tissue engineering AC is using the self-assembling process, where mature chondrocytes are harvested, dedifferentiated, and subsequently redifferentiated in vitro to produce a more hyaline-like AC [3][4][5]. Tissue engineering is inherently time-, resource-, and cost-intensive, and the destructive testing used to verify and validate tissue-engineered AC can be inappropriate or impractical for scale-up and biomanufacturing. Therefore, nondestructive methods to assess the evolution of mechanical properties and correlate them back to structure and composition are needed to further the clinical translation of TEMPs.The structure and composition of tissues has been independently evaluated by linear and nonlinear optics-based imaging techniques (e.g., second-harmonic generation (SHG) [6][7][8][9], Highlights Optics-based nondestructive techniques have shown to produce corroborative and congruent results to traditional tests.

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“…In this implementation, a 4f lens relay produces a scan at the input of the borescope which is then relayed to the sample plane at the distal end (figure 2(d)). The liquid lens allows electronic control of the beam focus, a practical way to adjust the optical imaging distance from the end of the borescope [85].…”

Section: Scanning Optics For Handheld Probesmentioning

confidence: 99%

A review of low-cost and portable optical coherence tomography

et al. 2021

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Optical coherence tomography (OCT) is a powerful optical imaging technique capable of visualizing the internal structure of biological tissues at near cellular resolution. For years, OCT has been regarded as the standard of care in ophthalmology, acting as an invaluable tool for the assessment of retinal pathology. However, the costly nature of most current commercial OCT systems has limited its general accessibility, especially in low-resource environments. It is therefore timely to review the development of low-cost OCT systems as a route for applying this technology to population-scale disease screening. Low-cost, portable and easy to use OCT systems will be essential to facilitate widespread use at point of care settings while ensuring that they offer the necessary imaging performances needed for clinical detection of retinal pathology. The development of low-cost OCT also offers the potential to enable application in fields outside ophthalmology by lowering the barrier to entry. In this paper, we review the current development and applications of low-cost, portable and handheld OCT in both translational and research settings. Design and cost-reduction techniques are described for general low-cost OCT systems, including considerations regarding spectrometer-based detection, scanning optics, system control, signal processing, and the role of 3D printing technology. Lastly, a review of clinical applications enabled by low-cost OCT is presented, along with a detailed discussion of current limitations and outlook.

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Optical coherence tomography through a rigid borescope applied to quantification of articular cartilage thickness in a porcine knee model

Cited by 5 publications

References 19 publications

Optical coherence tomography of small intestine allograft biopsies using a handheld surgical probe

Optical coherence tomography of small intestine allograft biopsies using a handheld surgical probe

Nondestructive testing of native and tissue-engineered medical products: adding numbers to pictures

A review of low-cost and portable optical coherence tomography

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