Optical Coherence Tomography: History, Current Status, and Laboratory Work

Gabriele, M. L.; Wollstein, Gadi; Ishikawa, Hiroshi; Kagemann, Larry; Xu, Juan; Folio, Lindsey S.; Schuman, Joel S.

doi:10.1167/iovs.10-6312

Cited by 249 publications

(182 citation statements)

References 177 publications

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“…Depending on the physical properties of OCT device and the reWnement of processing method, the image resolution can achieve the quality of a real histological slide [17,18]. OCT is based on the optical interferometry, typically using near-infrared light [17,18]. The basic two-dimensional images are processed by fast Fourier-transformation (FFT) of the collected light reXection and absorption data [17,18].…”

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confidence: 99%

“…OCT is based on the optical interferometry, typically using near-infrared light [17,18]. The basic two-dimensional images are processed by fast Fourier-transformation (FFT) of the collected light reXection and absorption data [17,18]. The relatively long wavelength light allows a 1-2 mm penetration into the scattering medium, because at greater depths, a large amount of emitted light escapes without scattering and reXection [17].…”

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confidence: 99%

See 2 more Smart Citations

Optical coherence tomography for biofilm detection in chronic rhinosinusitis with nasal polyposis

Tóth

Vajas

Csomor

et al. 2012

Eur Arch Otorhinolaryngol

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Optical coherence tomography for biofilm detection in chronic rhinosinusitis with nasal polyposis

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et al. 2012

Eur Arch Otorhinolaryngol

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“…[1][2][3] In 2014, at International Nomenclature OCT (INOCT) Panel, Staurenghi et al 4 proposed a nomenclature system for normal anatomic landmarks in SD-OCT and it has been reached a consensus regarding the most proper terminology and normal anatomic landmarks in the posterior segment SD-OCT. In addition, it has been recommended as the standardised nomenclature for use in the future publication in the panel.…”

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Past and Present Terminology for the Retinal and Choroidal Structures in Optical Coherence Tomography

Turgut¹

2017

European Ophthalmic Review

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Spectral domain optical coherence tomography (SD-OCT) is a non-invasive imaging method which is used in the diagnosis and followup of various macular diseases. SD-OCT provides detailed imaging of the retina. However, it has also been used to evaluate the choroidal layers. There are many publications on the OCT terminology, the definition and classification of retinal and choroidal structures including lines, bands and zones described in OCT. The aim of this review is to provide an overview of the literature on the past and present terminology for the retinal and choroidal structures seen in SD-OCT. To know OCT terminology will provide to be understanding better the pathogenesis of these diseases and the effects of therapeutic applications for these. KeywordsOptical coherence tomography, terminology, international nomenclature Disclosure: Burak Turgut has nothing to disclose with any trade or device in relation to this article. No funding was received for the publication of this article. This study involves a review of the literature and did not involve any studies with human or animal subjects performed by any of the authors.Authorship: All named authors meet the International Committee of Medical Journal Editors (ICMJE) criteria for authorship of this manuscript, take responsibility for the integrity of the work as a whole, and have given final approval to the version to be published.Open Access: This article is published under the Creative Commons Attribution Noncommercial License, which permits any non-commercial use, distribution, adaptation and reproduction provided the original author(s) and source are given appropriate credit. In this review, based on the literature, it has been aimed to provide an overview of the definition and classification of the retinal and choroidal structures seen in SD-OCT, and to propose the standard nomenclature and terminology for these structures.To know the INOCT, OCT terminology and nomenclature of retinal and choroidal layers, the landmarks, zones, bands and lines described in OCT in healthy eyes will also provide to be understanding better the pathogenesis of various macular diseases and the effects of therapeutic applications for these.

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“…While alternative microscopy-scale methods, such as confocal, two-photon, or optical coherence tomography approaches [2][3][4][5][6], can generate reasonable images from thick tissues, such instrumentation is typically complex, expensive, and not widely available. With MUSE, thick pieces of tissue obtained by biopsy, surgery or necropsy can be imaged directly, eliminating any requirement for conventional histology processing, formalin-fixation, paraffin-embedding, or thin-sectioning.…”

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Slide-Free Microscopy via UV Surface Excitation

et al. 2016

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Microscopic evaluation of tissue remains the definitive diagnostic procedure in the evaluation of most solid tumors, as well as of many other disease processes. Conventional brightfield or fluorescence microscopy works best with thin, stained specimens mounted on glass slides, but to prepare these requires hours of processing and the help of highly skilled technical personnel. We describe a new, inexpensive form of light microscopy, based on UV surface excitation (MUSE), that can generate highquality histology and histopathology images directly from cut surfaces of fresh (or fixed) tissue samples of any thickness, with less than 1 minute of preparation. Unlike standard diagnostic processes, it is nondestructive, preserving the specimen for downstream molecular analyses. Preliminary description of this method, which depends on the shallow tissue penetration of sub-300-nm light, can be found in [1].While alternative microscopy-scale methods, such as confocal, two-photon, or optical coherence tomography approaches [2-6], can generate reasonable images from thick tissues, such instrumentation is typically complex, expensive, and not widely available. With MUSE, thick pieces of tissue obtained by biopsy, surgery or necropsy can be imaged directly, eliminating any requirement for conventional histology processing, formalin-fixation, paraffin-embedding, or thin-sectioning. The method is rapid, and by providing diagnostic-level information in minutes, can potentially change the velocity of clinical encounters; Moreover, MUSE can be more informative than conventional histology, because it provides information on surface topography, typically effaced by thin-sectioning procedures. Figure 1 illustrates the basic MUSE configuration, which is based on an inverted microscope design, largely because it is the easiest way to position large, variably shaped tissue specimens. The sample rests on (or is lightly compressed against) a UV-transmitting flat optical surface, typically quartz or sapphire (replacing the coverslip in conventional microscopy). The excitation light is provided by one or more UV-emitting LEDs with maximum emission wavelength centered around 275-285 nm, arranged to illuminate the sample in oblique configuration. We typically use a 10X lens and a scientific-grade 9-Mpixel color (RGB) CCD camera.Samples need only be cut with a sharp knife or scalpel to provide a flat imaging surface, and then briefly stained. A convenient dye combination includes rhodamine and Hoechst in phosphate-buffered saline. The specimen is dipped into this mixture for 10 s and then washed gently in more PBS, and then can be immediately positioned on the UV-transparent stage for imaging. Depending on the specimen and the brightness of the LED(s) used, images can be acquired in as little as 100 ms per field. Multiple fields can be taken by translating the stage, and then montaged into a single, large field-of-view final image file.MUSE images are acquired in fluorescence and take advantage of the fact that many fluorescent dyes can all be ...

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Optical Coherence Tomography: History, Current Status, and Laboratory Work

Cited by 249 publications

References 177 publications

Optical coherence tomography for biofilm detection in chronic rhinosinusitis with nasal polyposis

Optical coherence tomography for biofilm detection in chronic rhinosinusitis with nasal polyposis

Past and Present Terminology for the Retinal and Choroidal Structures in Optical Coherence Tomography

Slide-Free Microscopy via UV Surface Excitation

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