Optical coherence tomography for complex diagnosis of vulvar diseases

Sirotkina, Marina A.; Potapov, Arseniy L.; Konovalova, Elizaveta A.; Vagapova, Nailya N.; Safonov, Ivan K.; Timakova, A.A.; Раденска-Лоповок, С Г; Kuznetsova, Irina A.; Karashtin, D. A.; Matveev, Lev A.; Moiseev, Alexander A.; Gelikonov, Grigory V.; Zagaynova, Elena V.; Gladkova, Natalia D.

doi:10.1117/12.2545801

Cited by 1 publication

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“…The purpose of this study is the description of polymorphic changes in connective tissue of VLS at different lesion degrees using MPM. Our research group plans to use the efforts we have made to isolate the degrees of dermal remodeling in VLS in the future for the development of an intravital high‐resolution imaging method—multimodal optical coherence tomography [35–37] and its use in the diagnosis and monitoring of VLS treatment.…”

Section: Introductionmentioning

confidence: 99%

Multiphoton microscopy assessment of the structure and variability changes of dermal connective tissue in vulvar lichen sclerosus: A pilot study

Potapov

Sirotkina

Matveev

et al. 2022

Journal of Biophotonics

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In this article, we offer a novel classification of progressive changes in the connective tissue of dermis in vulvar lichen sclerosus (VLS) relying on quantitative assessment of the second harmonic generation (SHG) signal received from formalin fixed and deparaffinized tissue sections. We formulate criteria for distinguishing four degrees of VLS development: Initial‐Mild‐Moderate‐Severe. Five quantitative characteristics (length and thickness type I Collagen fibers, Mean SHG signal intensity, Skewness and Coherence SHG signal) are used to describe the sequential degradation of connective tissue (changes in the structure, orientation, shape and density of collagen fibers) up to the formation of specific homogeneous masses. Each of the degrees has a characteristic set of quantitatively expressed features. We focus on the identification and description of early, initial changes of the dermis as the least specific. The results obtained by us and the proposed classification of the degrees of the disease can be used to objectify the dynamics of tissue changes during treatment.

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Section: Introductionmentioning

confidence: 99%