Optical coherence tomography‐based angiography device with real‐time angiography B‐scans visualization and hand‐held probe for everyday clinical use

Moiseev, Alexander A.; Ksenofontov, S. Yu.; Sirotkina, Marina A.; Kiseleva, Elena B.; Gorozhantseva, Maria; Shakhova, Natalia M.; Matveev, Lev A.; Zaitsev, Vladimir Yu.; Matveyev, Alexander L.; Zagaynova, Elena V.; Gelikonov, Valentin M.; Gladkova, Natalia D.; Vitkin, I. Alex; Gelikonov, Grigory V.

doi:10.1002/jbio.201700292

Cited by 56 publications

(69 citation statements)

References 28 publications

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“…22,23 Similar functional OCT approaches are being investigated for detection, quantification, and management of early RT toxicities as well, for example in intrafraction monitoring of mucositis development in superficial mucosal layers of the oral cavity of head and neck radiotherapy patients. [52][53][54] Further, skin is indeed a very important target of such RT imaging studies, given its clinical importance as a dose-limiting organ and thus common occurrence of RIF. However, its thicker structures in humans compared to mice may somewhat limit clinical utility, as OCT can only detect important RIF changes within ∼1 mm of the skin surface (unless optical clearing is used, a promising practical solution to this obstacle), 55 thus its application should be driven by relevant tissue changes within this depth range.…”

Section: Statisticsmentioning

confidence: 99%

Preclinical quantitative in-vivo assessment of skin tissue vascularity in radiation-induced fibrosis with optical coherence tomography

et al. 2018

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Radiation therapy (RT) is widely and effectively used for cancer treatment but can also cause deleterious side effects, such as a late-toxicity complication called radiation-induced fibrosis (RIF). Accurate diagnosis of RIF requires analysis of histological sections to assess extracellular matrix infiltration. This is invasive, prone to sampling limitations, and thus rarely used; instead, current practice relies on subjective clinical surrogates, including visual observation, palpation, and patient symptomatology questionnaires. This preclinical study demonstrates that functional optical coherence tomography (OCT) is a useful tool for objective noninvasive in-vivo assessment and quantification of fibrosis-associated microvascular changes in tissue. Data were collected from murine hind limbs 6 months after 40-Gy single-dose irradiation and compared with nonirradiated contralateral tissues of the same animals. OCT-derived vascular density and average vessel diameter metrics were compared to quantitative vascular analysis of stained histological slides. Results indicate that RIF manifests significant microvascular changes at this time point posttreatment. Abnormal microvascular changes visualized by OCT in this preclinical setting suggest the potential of this label-free high-resolution noninvasive functional imaging methodology for RIF diagnosis and assessment in the context of clinical RT.

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Section: Statisticsmentioning

confidence: 99%

Preclinical quantitative in-vivo assessment of skin tissue vascularity in radiation-induced fibrosis with optical coherence tomography

et al. 2018

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“…Since the proposed method of numerical refocusing operates with the fullscale data of the scattered field measured with an OCT device, the resulting image is sensitive to phase deviations that could be caused by movements of the object and/or the OCT scanner during the measurement. To compensate for the possible phase deviations, we used the method described in [22].…”

Section: Methodsmentioning

confidence: 99%

Improving the Transverse Resolution of Optical Coherence Tomography with a Finite Impulse Response Filter and a Series of Numerically Refocused Images

Moiseev¹,

Gelikonov²,

Ksenofontov³

et al. 2019

Sovrem Tehnol Med

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Among the numerous methods for improving the informative value of the optical coherence tomography (OCT), a special place is taken by the methods for increasing the spatial resolution of the resulting images. Increasing the resolution allows one to identify more clinically significant structures in OCT images and thus improve the diagnostic value of OCT. Since the transverse resolution of OCT images is determined by the physical principles different from those for the longitudinal resolution, the ways of their improvement are also different. The aim of the study is to develop a method for increasing the transverse resolution of OCT by using a finite impulse response (FIR) filter for numerical refocusing and by combining the numerically refocused images. Results. We have developed a FIR filter able to transfer the focal plane of an OCT image using the data from 17 consecutive measurements of the field scattered by the object. In addition, we propose an original method for automatic synthesis of the final OCT image with an improved transverse resolution over the entire object depth from a series of images having different focal plane positions. This will enable the OCT instrument to produce a sharply focused beam for the scanning and thus obtain images with an improved transverse resolution in the focal plane, restore the required resolution in the out-of-focus areas using numerical transfer of the focal plane, and also synthesize the final OCT image with an improved transverse resolution. The method has been tested with model objects using an OCT device operating at a central wavelength of 1 μm with the 60 nm range and a beam focused onto a spot with a diameter of 5 μm. Conclusion. The proposed method of numerically increasing the transverse resolution of OCT images allows one to obtain images with an improved transverse resolution using only a small number of OCT measurements; in the future, it will allow for creating an OCT device that produces high-resolution images in real time.

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“…2(a-c) (in the absence of additive noise), but for randomly distributed 5 10 2  scatterers, the density of which is close to concentration of cells in biological tissues. In the simulation, the axially moved particles displaced by 2.25x10 -4 m between the consequent A-scans, which corresponds to the velocity 2.25 m/s for the acquisition rate of the OCT scanner described in [9,24] and applied for angiographic mapping in [25,26,27,28] using the high-pass filtering principle. Figure 3a shows the initial B-scan through the axis of the vertical flow and Fig.…”

Section: Examples Of Refocusing Of Simulated Oct Scans Including the mentioning

confidence: 99%

Computationally efficient model of OCT scan formation by focused beams and its usage to demonstrate a novel principle of OCT-angiography

Matveyev¹,

Matveev²,

Moiseev³

et al. 2020

Laser Phys. Lett.

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A computationally highly efficient full-wave model of OCT-scan formation by focused beams in spectral-domain optical coherence tomography (OCT) is presented. Similarly to some previous models, it is based on summation of fields scattered by discrete sub-resolution scatterers and enables accounting for axial and lateral inhomogeneity of the illuminating Gaussian beam. The main feature ensuring high computational efficiency of the described model is that instead of numerical integration of the scattered signal over the receiving aperture we apply analytical description of both the illuminating-beam focusing and collection of the scattered signals over the receiving aperture. Elimination of numerical integration over the receiving aperture has increased the computation speed by a factor of ~3 10. This is of key importance for practical feasibility of simulations of 3D OCT data volumes for large amounts (~7 5 10 10 ) of scatterers corresponding to realistic densities of cells in biological tissues. We demonstrate the model possibilities by simulating digital refocusing of strongly focused OCT beams in the presence moving scatterers. A novel principle of contrast-agent-free visualization of scatterer flows with velocities typical of blood microcirculation is demonstrated.

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Optical coherence tomography‐based angiography device with real‐time angiography B‐scans visualization and hand‐held probe for everyday clinical use

Cited by 56 publications

References 28 publications

Preclinical quantitative in-vivo assessment of skin tissue vascularity in radiation-induced fibrosis with optical coherence tomography

Preclinical quantitative in-vivo assessment of skin tissue vascularity in radiation-induced fibrosis with optical coherence tomography

Improving the Transverse Resolution of Optical Coherence Tomography with a Finite Impulse Response Filter and a Series of Numerically Refocused Images

Computationally efficient model of OCT scan formation by focused beams and its usage to demonstrate a novel principle of OCT-angiography

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