Optical coherence tomography allows for the reliable identification of laryngeal epithelial dysplasia and for precise biopsy: A clinicopathological study of 61 patients undergoing microlaryngoscopy

Just, Tino; Lankenau, Eva; Prall, Friedrich; Hüttmann, Gereon; Pau, Hans Wilhelm; Sommer, K.

doi:10.1002/lary.21057

Cited by 26 publications

(22 citation statements)

References 16 publications

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“…Quantitative analysis of cellular and morphological features, e.g. nuclear size, nuclear density, number of nuclei per cell, nucleus/cytoplasm ratio, regularity of the cell layers, morphology of cells in a cell layer, and occurrence of cellular junctions, showed good agreement with histology, suggesting that LSM can identify pathologies in vivo (Just et al, 2006b). Confocal microscopy of the healthy human larynx revealed that the border between the superficial and basement cell layer is clearly visible, while the basement membrane itself cannot be detected (Fig.…”

Section: Introductionmentioning

confidence: 82%

“…4. Good correlation between LSM images and conventional histology were achieved in normal tissue, but also in benign and cancerous lesions of the larynx (Just et al, 2006b;Just et al, 2007b) and oropharynx (Just et al, 2008;White et al, 1999). 5.…”

Section: Introductionmentioning

confidence: 97%

“…Next, we commenced ex-vivo studies of laryngeal mucosa (Figures 2-7). A pre-clinical trial has been carried out to assess benign, pre-malignant and malignant lesions of the human larynx (Just et al, 2006b;Just et al, 2007b). Quantitative analysis of cellular and morphological features, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…2. LSM provides longitudinal-sections of the tissue and allows for characterization of normal laryngeal epithelium and of laryngeal lesions from different histogenetic origins (Just et al, 2006b;Just et al, 2007b).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Rigid Confocal Endoscopy of the Larynx

Just¹,

Stave²,

Stachs³

et al. 2011

Laser Scanning, Theory and Applications

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Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rigid Confocal Endoscopy of the Larynx

Just¹,

Stave²,

Stachs³

et al. 2011

Laser Scanning, Theory and Applications

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“…(15) There have been studies investigating the use of OCT in other medical fields, such as cardiology, gastroenterology, (16) mastology, (17) pulmonology, urology, (18) and otolaryngology. (19) Because it enables high-resolution real-time imaging without exposure to radiation and because it is compatible with bronchoscopy, OCT has the potential to become a powerful tool in future lung research. (20) Minimally invasive visualization of tissue anatomy provides important information for the diagnosis and clinical management of a wide range of diseases.…”

Section: Discussionmentioning

confidence: 99%

Tomografia de coerência óptica broncoscópica

et al. 2012

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Objective: To evaluate the feasibility of and the potential for using optical coherence tomography in conjunction with conventional bronchoscopy in the evaluation of the airways. Methods: This was a pilot study based on an ex vivo experimental model involving three animals: one adult New Zealand rabbit and two Landrace pigs. An optical coherence tomography imaging catheter was inserted through the working channel of a flexible bronchoscope in order to reach the distal trachea of the animals. Images of the walls of the trachea were systematically taken along its entire length, from the distal to the proximal portion. Results: The imaging catheter was easily adapted to the working channel of the bronchoscope. High-resolution images of cross sections of the trachea were taken in real time, precisely delineating microstructures, such as the epithelium, submucosa, and cartilage, as well as the adventitia of the anterior and lateral tracheal walls. The corresponding layers of the epithelium, mucosa, and cartilage were clearly differentiated. The mucosa, submucosa, and trachealis muscle were clearly identified in the posterior wall. Conclusions: It is feasible to use an optical coherence tomography imaging catheter in combination with a flexible bronchoscope. Optical coherence tomography produces highresolution images that reveal the microanatomy of the trachea, including structures that are typically seen only on images produced by conventional histology.Keywords: Tomography, optical coherence; Trachea; Bronchoscopy; Microscopy; Diagnostic techniques, respiratory system. ResumoObjetivo: Avaliar a viabilidade e o potencial do uso da tomografia de coerência óptica em conjunto com um broncoscópio convencional na avaliação das vias aéreas. Métodos: Estudo piloto baseado em um modelo experimental ex vivo com três animais: um coelho adulto da raça Nova Zelândia e dois suínos da raça Landrace. Um cateter de imagem de tomografia de coerência óptica foi inserido no canal de trabalho de um broncoscópio flexível para alcançarmos a traqueia distal dos animais. As imagens foram obtidas sistematicamente em toda a traqueia ao longo das paredes, partindo da porção distal para a proximal. Resultados: O cateter de imagem se adaptou com facilidade ao canal de trabalho do broncoscópio. Imagens em alta resolução de cortes transversais da traqueia foram obtidas em tempo real, sendo delineadas microestruturas, tais como epitélio, submucosa, cartilagem e camada adventícia nas paredes anteriores e laterais da traqueia. As camadas correspondentes do epitélio, mucosa e cartilagens foram claramente diferenciadas. Na parede posterior, foi possível identificar mucosa, submucosa e musculatura traqueal. Conclusões: O uso de tomógrafo de coerência óptica em conjunto com um broncoscópio flexível é viável. A tomografia de coerência óptica produz imagens de alta resolução que permitem visualizar a microanatomia da traqueia, inclusive estruturas que normalmente são visualizadas somente na histologia convencional.Descritores: Tomografia de coerênc...

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Hyperspectral imaging of mucosal surfaces in patients

Gerstner

Laffers

Bootz

et al. 2012

Journal of Biophotonics

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The aim of this study was to proof applicability of hyperspectral imaging for the analysis and classification of human mucosal surfaces in vivo. The larynx as a prototypical anatomically well-defined surgical test area was analyzed by microlaryngoscopy with a polychromatic lightsource and a synchronous triggered monochromatic CCD-camera. Image stacks (5 benign, 7 malignant tumors) were analyzed by established software (principal component analysis PCA, hyperspectral classification, spectral profiles). Hyperspectral image datacubes were analyzed and classified by conventional software. In PCA, images at 590-680 nm loaded most onto the first PC which typically contained 95% of the total information. Hyperspectral classification clustered the data highlighting altered mucosa. The spectral profiles clearly differed between the different groups. Hyperspectral imaging can be applied to mucosal surfaces. This approach opens the way to analyze spectral characteristics of histologically different lesions in order to build up a spectral library and to allow non-touch optical biopsy.

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Optical coherence tomography allows for the reliable identification of laryngeal epithelial dysplasia and for precise biopsy: A clinicopathological study of 61 patients undergoing microlaryngoscopy

Abstract: OCT allows for a fairly accurate assessment of benign and dysplastic laryngeal epithelial lesion and greatly facilitates the taking of precise biopsies. Laryngoscope, 2010.

Cited by 26 publications

References 16 publications

Rigid Confocal Endoscopy of the Larynx

Rigid Confocal Endoscopy of the Larynx

Tomografia de coerência óptica broncoscópica

Hyperspectral imaging of mucosal surfaces in patients

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