Optical coherence tomography: a reliable alternative to invasive histological assessment of acute wound healing in human skin?

Greaves, Nicholas S.; Benatar, Brian; Whiteside, Sigrid; Alonso-Rasgado, Teresa A; Baguneid, Mohamed; Bayat, Ardeshir

doi:10.1111/bjd.12786

Cited by 45 publications

(58 citation statements)

References 28 publications

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“…The remodeling phase imaged here coincided with the completion of revascularization and the normalization of vascular parameters surrounding the wound area. Similar observations were noted previously (61). Additionally, the swirled dermal patterns observed here have been linked previously to collagen type III being converted to type I (61,69).…”

Section: Discussionsupporting

confidence: 91%

“…Similar observations were noted previously (61). Additionally, the swirled dermal patterns observed here have been linked previously to collagen type III being converted to type I (61,69). This is corroborated by the bright dermis imaged immediately below the now healed wound indicating enhanced collagenous activity (65).…”

Section: Discussionsupporting

confidence: 90%

“…This is typical of partial thickness burns because as they extend to the dermis and damage blood vessels, the immediate response of the microcirculation is to cause coagulation at the wound site and hyperemia in the surrounding areas (60). A similar hyperemic response was noted previously with OCT (61). At this point in the healing process, the increased vasculature was not the result of angiogenesis; rather, the recruitment of pre-existing vessels that previously had little or no blood flow, which was below the minimal velocity that can be detected by OCTA (~4 µm/s) (62).…”

Section: Discussionsupporting

confidence: 57%

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Optical coherence tomography angiography monitors human cutaneous wound healing over time

Deegan¹,

Men²,

Li³

et al. 2018

Quant. Imaging Med. Surg.

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Background: In vivo imaging of the complex cascade of events known to be pivotal elements in the healing of cutaneous wounds is a difficult but essential task. Current techniques are highly invasive, or lack the level of vascular and structural detail required for accurate evaluation, monitoring and treatment. We aimed to use an advanced optical coherence tomography (OCT)-based angiography (OCTA) technique for the noninvasive, high resolution imaging of cutaneous wound healing.Methods: We used a clinical prototype OCTA to image, identify and track key vascular and structural adaptations known to occur throughout the healing process. Specific vascular parameters, such as diameter and density, were measured to aid our interpretations under a spatiotemporal framework.Results: We identified multiple distinct, yet overlapping stages, hemostasis, inflammation, proliferation, and remodeling, and demonstrated the detailed vascularization and anatomical attributes underlying the multifactorial processes of dermatologic wound healing.Conclusions: OCTA provides an opportunity to both qualitatively and quantitatively assess the vascular response to acute cutaneous damage and in the future, may help to ascertain wound severity and possible healing outcomes; thus, enabling more effective treatment options.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 57%

See 1 more Smart Citation

Optical coherence tomography angiography monitors human cutaneous wound healing over time

Deegan¹,

Men²,

Li³

et al. 2018

Quant. Imaging Med. Surg.

View full text Add to dashboard Cite

show abstract

“…Furthermore, previous research also suggests conventional OCT to be useful to assess acute wound healing in human skin by obtaining the mean gray scale value (MGV; i.e., intensity level). 7,28 In these studies, the MGV was significantly increased 6 weeks after wounding and OCT assessed architectural changes corresponded with the different phases of wound healing (i.e., inflammatory, proliferative, and remodeling phase) as evaluated histologically, suggesting that conventional OCT can assess the amount of fibrosis and monitor wound healing noninvasively.…”

Section: Discussionmentioning

confidence: 94%

“…6,7 With polarization-sensitive OCT (PS-OCT), the polarimetric properties of a sample can be determined. For example, when light travels through a birefringent sample, its polarization state changes.…”

Section: Introductionmentioning

confidence: 99%

In vivo polarization-sensitive optical coherence tomography of human burn scars: birefringence quantification and correspondence with histologically determined collagen density

Jaspers

Feroldi²,

Vlig

et al. 2017

J. Biomed. Opt.

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In vivo polarization-sensitive optical coherence tomography of human burn scars: birefringence quantification and correspondence with histologically determined collagen density," J. Biomed. Opt. Abstract. Obtaining adequate information on scar characteristics is important for monitoring their evolution and the effectiveness of clinical treatment. The aberrant type of collagen in scars may give rise to specific birefringent properties, which can be determined using polarization-sensitive optical coherence tomography (PS-OCT). The aim of this pilot study was to evaluate a method to quantify the birefringence of the scanned volume and correlate it with the collagen density as measured from histological slides. Five human burn scars were measured in vivo using a handheld probe and custom-made PS-OCT system. The local retardation caused by the tissue birefringence was extracted using the Jones formalism. To compare the samples, histograms of birefringence values of each volume were produced. After imaging, punch biopsies were harvested from the scar area of interest and sent in for histological evaluation using Herovici polychrome staining. Two-dimensional en face maps showed higher birefringence in scars compared to healthy skin. The Pearson's correlation coefficient for the collagen density as measured by histology versus the measured birefringence was calculated at r ¼ 0.80 (p ¼ 0.105). In conclusion, the custom-made PS-OCT system was capable of in vivo imaging and quantifying the birefringence of human burn scars, and a nonsignificant correlation between PS-OCT birefringence and histological collagen density was found.

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High‐Resolution Optical Coherence Tomography (OCT) for Skin Imaging

Wang

et al. 2019

Imaging Technologies and Transdermal Delivery in Skin Disorders

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Optical coherence tomography: a reliable alternative to invasive histological assessment of acute wound healing in human skin?

Abstract: Optical coherence tomography enabled accurate assessment of healing tissue comparable with histological analysis of biopsy specimens. This noninvasive tool is highly suited to wound assessment and may represent a diagnostic alternative to punch biopsies.

Cited by 45 publications

References 28 publications

Optical coherence tomography angiography monitors human cutaneous wound healing over time

Optical coherence tomography angiography monitors human cutaneous wound healing over time

In vivo polarization-sensitive optical coherence tomography of human burn scars: birefringence quantification and correspondence with histologically determined collagen density

High‐Resolution Optical Coherence Tomography (OCT) for Skin Imaging

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