Optical clearing agent increases effectiveness of photodynamic therapy in a mouse model of cutaneous melanoma: an analysis by Raman microspectroscopy

Martinelli, Letícia Palombo; Iermak, Ievgeniia; Moriyama, Lílian Tan; Requena, Michelle Barreto; Pires, Layla; Kurachi, Cristina

doi:10.1364/boe.405039

Cited by 10 publications

(4 citation statements)

References 52 publications

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“…imposed in light treatments. Amidst possible solutions that could increase the efficacy of PDT, one can mention the offset illumination of tissue, 1 the application of optical clearing agents, 42 and the utilization of mechanical waves, more specifically, ultrasonic waves.…”

Section: Light Propagation In Skinmentioning

confidence: 99%

Theoretical study of the conditions for acoustic nucleation and cavitation in biological tissue for its application in sono-photodynamic therapy

Aparecida Antunes,

Tovar,

Pratavieira

2024

Clinical Biophotonics III

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Photodynamic therapy (PDT) is a therapeutic modality that combines light, a light-activated drug, and the molecular oxygen of the medium to disrupt cells. Due to the low penetration of light in biological tissue and its absorption by pigmented cells, such as melanoma, the effective application of PDT is usually limited to superficial and non-pigmented lesions. The utilization of ultrasound-based therapies, such as Sonodynamic therapy (SDT) and Sono-photodynamic Therapy (SPDT), poses a possible solution to overcome these limitations. Besides the greater penetration of acoustic waves in the tissue, the propagation of ultrasonic waves can form gas/vapor bubbles in the medium and cause its subsequent oscillation and implosion, known as acoustic nucleation and cavitation. The implosion of oscillating bubbles can generate sonomechanical and sonochemical effects, such as the production of reactive oxygen species (ROS) by pyrolysis and sonoluminescence, leading to tumor death. This study aims to obtain, in a first approximation, the profile of light and ultrasound penetration in skin and melanoma cells, as well as the increase in temperature generated by the implosion of cavitated bubbles. For this purpose, the Monte Carlo eXtreme (MCX) and k-wave toolbox for MATLAB were used to obtain the beam profile of light and ultrasound, respectively, in skin and melanoma. For obtaining the inertial cavitation threshold and the peak of temperature due to the bubble's implosion, the Keller-Miksis (KM) equation was solved in the software Wolfram Mathematica. While the results were obtained from simplified models, they corroborate the greater penetration of ultrasound (US) in skin and melanoma when compared to light. While light at a wavelength (λ) equal to 630 nm reaches less than 3 mm within the tissue, US waves at the frequency of 1 MHz and acoustic pressure equal to 0.32 MPa can penetrate 15 mm, and the acoustic intensity is smoothly reduced in melanoma. Furthermore, the implosion of the cavitated bubbles can generate a local increase in temperature up to 1000 K, which can be responsible for the generation of ROS. Despite the need for more refined models to describe these phenomena with greater precision, they demonstrate that the utilization of SDT and SPDT can be a good approach for the effective and noninvasive treatment of melanoma.

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Section: Light Propagation In Skinmentioning

confidence: 99%

Theoretical study of the conditions for acoustic nucleation and cavitation in biological tissue for its application in sono-photodynamic therapy

Aparecida Antunes,

Tovar,

Pratavieira

2024

Clinical Biophotonics III

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“…The novelty of OTC-3D medical application imaging is the use of entire biopsies marked by specific fluorescent probes, making it a cost-effective, nonradioactive mode of imaging for cancer detection (Martinelli 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Recent findings in OTC-3D imaging are focused on the improvement of near-infrared probes. This spectrum range (700–850 nm) permits absorption of light deeper than others emitting at 400–600 nm, allowing a better tissue visualization and resulting in lower light absorption and minor autofluorescence by blood and other components in this spectrum range (Martinelli 2020 ). Moreover, cancer tissues are often characterized by necrotic areas, which might not be imaged in standard conditions.…”

Section: Introductionmentioning

confidence: 99%

Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies

Brenna

Simioni

Varano

et al. 2022

Histochem Cell Biol

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Understanding the inner morphology of intact tissues is one of the most competitive challenges in modern biology. Since the beginning of the twentieth century, optical tissue clearing (OTC) has provided solutions for volumetric imaging, allowing the microscopic visualization of thick sections of tissue, organoids, up to whole organs and organisms (for example, mouse or rat). Recently, tissue clearing has also been introduced in clinical settings to achieve a more accurate diagnosis with the support of 3D imaging. This review aims to give an overview of the most recent developments in OTC and 3D imaging and to illustrate their role in the field of medical diagnosis, with a specific focus on clinical applications. Graphical abstract

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“…76 proporcionou uma resposta mais uniforme até 725 μm de profundidade na espessura dos tecidos. 77 O Clorina e6, um agente fotossensibilizante de segunda geração derivado de clorina natural, apresenta absorção significativa na região do vermelho do espectro luminoso, dentro da chamada janela "terapêutica" (600-800 nm). Além disso, possui eficiente cruzamento intersistemas, possibilitando a geração efetiva de oxigênio singlete (1O 2 ), um fator crucial para a indução da morte celular tumoral na Terapia Fotodinâmica (TFD).…”

Section: Terapia Fotodinâmica (Tfd)unclassified

Investigação da morte de células B16F10 >i<in vitro>/i< com associação de radioterapia e terapia fotodinâmica

Lopes

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Dedico este trabalho à minha família e ao meu amor, Nayra, que me apoiaram tornando esta conquista possível. AGRADECIMENTOSAgradeço à Deus por me conceder a conclusão de mais uma etapa da minha vida e por fazer-se presente todo tempo.Aos meus familiares. Aqui agradeço minha mãe: mulher guerreira, meu espelho e fortaleza. Meus avós, pelo amor incondicional. Meu irmão, pela parceria e apoio. Meus tios Rogério e João Carlos sempre estiveram prontos para ajudar, não importa a dificuldade em que eu me encontrasse.À Ma. Nayra, meu amor e companheira de vida, agradeço por me ajudar por todo o mestrado com revisões e sugestões, me apoiando em todos os momentos principalmente nos momentos mais críticos e sempre acreditando em mim.À Drª Natalia Inada, minha orientadora e amiga, por ter aberto as portas mim no IFSC. Obrigado por seus ensinamentos, seus conselhos e conversas, sua atenção, ajuda e por construir junto comigo esse caminho.Ao Giancarlo, meu ex companheiro de laboratório. Obrigado por seu apoio e pela amizade que começou no IFSC e continua fora dele.Ao Johan, pela amizade, conselhos e as piadas que deixaram os dias mais leves.À Claudinha, pelas conversas divertidas e sempre estar disposta a dividir um cafezinho.À Letícia, minha "mãe de laboratório", obrigado pelas dicas, ajudas e ensinamentos dentro laboratório. Pelas sugestões e revisões no texto Às amigas, Camilla Costa, Bretas, Malu, Camila Antunes e Lorraine, obrigado pela amizade, pelos "rolês", pelas conversas e trocas e sempre me incentivarem.Aos amigos da sala 35, Tiago, Maju, Rebeca, Gabriel, AnaJu, Loraine, obrigado pelas conversas, pelos bandecos e pela hora da cachaça.À professora Cristina Kurachi, pela amizade, conselhos e conversas durante o café.À Neusa e a toda equipe da Biblioteca Prof. Bernhard Gross, do IFSC, não apenas pelo excelente suporte nas formatações, mas também pela atenção e cuidado em me tranquilizar.Aos funcionários da secretaria, Adriane, Benê, Cristiane e Wagner, pelo excepcional suporte administrativo. A disponibilidade e atenção foram fundamentais para que eu pudesse conduzir minha pesquisa de forma tranquila.Ao Prof Dr Vanderlei Salvado Bagnato, pelas discussões cientificas, ideias, motivações e todo imprescindível apoio financeiro.À Profª Drª Alexandra Ivo de Medeiros e seu aluno Breno Vilas Boas Raimundo, por me instruírem com os experimentos de detecção de morte celular.

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Optical clearing agent increases effectiveness of photodynamic therapy in a mouse model of cutaneous melanoma: an analysis by Raman microspectroscopy

Cited by 10 publications

References 52 publications

Theoretical study of the conditions for acoustic nucleation and cavitation in biological tissue for its application in sono-photodynamic therapy

Theoretical study of the conditions for acoustic nucleation and cavitation in biological tissue for its application in sono-photodynamic therapy

Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies

Investigação da morte de células B16F10 >i<in vitro>/i< com associação de radioterapia e terapia fotodinâmica

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