Optical characteristics of Agarose gel

Renmei, Zhang; Zhang, Jun; Mengyuan, Xie; Chen, Jiansu

doi:10.3788/irla201645.0721003

Cited by 4 publications

(2 citation statements)

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“…For single and multiphoton microscopy, a good signal to background ratio was achieved. Agarose and polyacrylamide gels have been extensively characterized using ultra-violet-visible and near infra-red (UV-Vis-NIR) and Raman spectroscopy, 30 – 33 and polyacrylamide gels are known to exhibit minimal autofluorescence in a limited wavelength region (350 to 500 nm), which was not used in this study, whereas agarose gels are generally considered non-fluorescent 34 – 37 For CARS imaging, only the symmetric methylene (CH2) stretching vibration was probed at 2845 cm−1.…”

Section: Resultsmentioning

confidence: 99%

Development of hydrogel-based standards and phantoms for non-linear imaging at depth

Haseeb,

Bourdakos,

Forsyth

et al. 2023

J. Biomed. Opt.

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Rapid advances in medical imaging technology, particularly the development of optical systems with non-linear imaging modalities, are boosting deep tissue imaging. The development of reliable standards and phantoms is critical for validation and optimization of these cutting-edge imaging techniques.Aim: We aim to design and fabricate flexible, multi-layered hydrogel-based optical standards and evaluate advanced optical imaging techniques at depth. Approach: Standards were made using a robust double-network hydrogel matrix consisting of agarose and polyacrylamide. The materials generated ranged from single layers to more complex constructs consisting of up to seven layers, with modality-specific markers embedded between the layers.Results: These standards proved useful in the determination of the axial scaling factor for light microscopy and allowed for depth evaluation for different imaging modalities (conventional one-photon excitation fluorescence imaging, two-photon excitation fluorescence imaging, second harmonic generation imaging, and coherent anti-Stokes Raman scattering) achieving actual depths of 1550, 1550, 1240, and 1240 μm, respectively. Once fabricated, the phantoms were found to be stable for many months. Conclusions:The ability to image at depth, the phantom's robustness and flexible layered structure, and the ready incorporation of "optical markers" make these ideal depth standards for the validation of a variety of imaging modalities.

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Section: Resultsmentioning

confidence: 99%

Development of hydrogel-based standards and phantoms for non-linear imaging at depth

Haseeb,

Bourdakos,

Forsyth

et al. 2023

J. Biomed. Opt.

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show abstract

“…A difference in humidity sensitivity existed between simulation and experiment. The difference may be because the light absorption of agarose gel [27] is about four times higher in the 2 µm waveband than the 1.5 µm waveband and changes in refractive index are nonlinear as a function of wavelength.…”

Section: Wavelength-based Modulationmentioning

confidence: 99%

Fiber sensor for relative humidity measurement in the water absorption band at 2 µm

Chen

Zhang

et al. 2019

Meas. Sci. Technol.

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An optical-fiber sensor based on a Mach-Zehnder interferometer is proposed and experimentally demonstrated for relative humidity (RH) measurement in the water absorption band at 2 µm. The sensor head is formed by a dual biconical fiber coated with agarose gel. Both the simulation and experimental results show that the proposed sensor is more sensitive to refractive index in the 2 µm waveband compared with the 1.5 µm waveband. Furthermore, we applied the sensor for humidity sensing. The results show that the sensor is very sensitive to humidity due to strong water absorption and weaker field confinement in the fiber core in the 2 µm waveband. The sensitivity achieved in our experiment was 231 pm/% RH, which was 3.5 times higher than for that sensor at 1.5 µm.

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Agarose coated macro-bend fiber sensor for relative humidity and temperature measurement at 2 μm

Chen

Zhang

et al. 2019

Optical Fiber Technology

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Optical characteristics of Agarose gel

Cited by 4 publications

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Development of hydrogel-based standards and phantoms for non-linear imaging at depth

Development of hydrogel-based standards and phantoms for non-linear imaging at depth

Fiber sensor for relative humidity measurement in the water absorption band at 2 µm

Agarose coated macro-bend fiber sensor for relative humidity and temperature measurement at 2 μm

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Optical characteristics of Agarose gel

Cited by 4 publications

References 0 publications

Development of hydrogel-based standards and phantoms for non-linear imaging at depth

Development of hydrogel-based standards and phantoms for non-linear imaging at depth

Fiber sensor for relative humidity measurement in the water absorption band at 2 µm

Agarose coated macro-bend fiber sensor for relative humidity and temperature measurement at 2 μm

Contact Info

Product

Resources

About

Agarose coated macro-bend fiber sensor for relative humidity and temperature measurement at 2 μm