Optical brighteners as thin-layer chromatographic detection reagents for glycoalkaloids and steroid alkaloids in Solanum species

Quantification of Light-Induced Glycoalkaloids, .ALPHA.-Solanine and .ALPHA.-Chaconine, in Four Potato Cultivars (Solanum tuberosum L.) Distributed in Japan by LC/MS

“…1) are detected in tissues of tubers except in the pith or center portion (Maga, 1980). It has been reported that these glycoalkaloids show acute toxicity (Jellema et al, 1980; McMillan & Thompson, 1980), chronic toxicity (Mun et al, 1975;Keeler et al, 1976), and have a bitter taste (Sinden & Deahl, 1976; Woolfe, 1987). The intrinsic glycoalkaloid content in tubers of commercial potato cultivars is low due to selection in breeding, but the content may increase in response to environmental stress conditions, such as light (Friedman, 1997;Griffiths et al, 1994;Kozukue et al, 1993;Maga, 1980;Percival & Dixon, 1994;Percival, 1999; Sinden et al, 1984; Woolfe, 1987), frost and hail damage, and wounding during harvesting or postharvest handling (Woolfe, 1987).…”

Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry of Solanidine, Leptinidine, and Acetylleptinidine. Steroidal Alkaloids from Solanum chacoense Bitter

“…Further, it has even been suggested that it may be possible to screen for deterrent glycoalkaloid content in lieu of resistance testing (Deahl and Sinden, 1987). Because of the potential ecological and toxicological significance of certain steroidal glycoalkaloids (McMillan and Thompson, 1979;Friedman, 1992), several methods have been developed to assay (glyco)alkaloid content in leaves and tubers of Solanum species (Coxon, 1984;Coxon and Jones, 1981;Crabbe and Fryer, 1980;Deahl and Sinden, 1987;Jellema et al, 1980Jellema et al, , 1981King, 1980;Lawson et al, 1992;Morris and Lee, 1981;Van Gelder, 1985).…”

Analysis of Solanum alkaloids using internal standardization and capillary gas chromatography

“…For glycoalkaloid analysis, GC is unattractive principally because of the high operating temperatures (>300 °C) necessary to achieve elution, even if samples are derivatized (Herb et al, 1975). TLC has been limited primarily to qualitative or semiquantitative analysis (Coxon and Jones, 1981; Deahl and Sinden, 1987;Hunter et al, 1976;Shih and Ku6,1974), although a few exceptions exist (Jellema et al, 1980(Jellema et al, ,1981. HPLC provides resolution of glycoalkaloids, but due to the absence of a good UV-absorbing chromophore, detection limits are 0.1 µ% at best (Morris and Lee, 1981).…”

“…These methods vary in their approach and include colorimetric assays (Bushway et al, 1980;Coxon et al, 1979;Fitzpatrick and Osman, 1974; Sanford and Sinden, 1972), thin-layer Vegetable Research Foundation; and state and federal funds appropriated to the Ohio Agricultural Research and Development Center (OARDC), The Ohio State University. Manuscript chromatography (TLC) (Coxon and Jones, 1981; Deahl and Sinden, 1987;Hunter et al, 1976;Jellema et al, 1980Jellema et al, , 1981; Shih and Ku6, 1974), high-performance liquid chromatography (HPLC) (Crabbe and Fryer, 1980;Morris and Lee, 1981), and gas chromatography (GC) (King, 1980;van Gelder, 1985). However, these chromatographic methods lack an accurate measure of glycoalkaloid recovery during sample preparation.…”