Optical Brain Imaging: A Powerful Tool for Neuroscience

Zhu, Xinpei; Xia, Yanfang; Wang, Xuecen; Si, Ke; Gong, Wei

doi:10.1007/s12264-016-0053-6

Cited by 21 publications

(15 citation statements)

References 66 publications

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“…The US-retrieval combined with β-AR antagonism could be a potential strategy for the treatment of memory disorders, such as substance addiction or post-traumatic stress disorders. The neuronal circuitry underlying memory reconsolidation triggered by US-retrieval or CS-retrieval should be studied in our future research (Zhu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Unconditioned- and Conditioned- Stimuli Induce Differential Memory Reconsolidation and β-AR-Dependent CREB Activation

Bing

Zhu

Zhou

et al. 2017

Front. Neural Circuits

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Consolidated long-term fear memories become labile and reconsolidated upon retrieval by the presentation of conditioned stimulus (CS) or unconditioned stimulus (US). Whether CS-retrieval or US-retrieval will trigger different memory reconsolidation processes is unknown. In this study, we introduced a sequential fear conditioning paradigm in which footshock (FS) was paired with two distinct sounds (CS-A and CS-B). The treatment with propranolol, a β-adrenergic receptor (β-AR) antagonist, after US (FS)-retrieval impaired freezing behavior evoked by either CS-A or CS-B. Betaxolol, a selective β1-AR antagonist, showed similar effects. However, propranolol treatment after retrieval by one CS (e.g., CS-A) only inhibited freezing behavior evoked by the same CS (i.e., CS-A), not the other CS (CS-B). These data suggest that β-AR is critically involved in reconsolidation of fear memory triggered by US- and CS-retrieval, whereas β-AR blockade after US-retrieval disrupts more CS-US associations than CS-retrieval does. Furthermore, significant CREB activation in almost the whole amygdala and hippocampus was observed after US-retrieval, but CS-retrieval only stimulated CREB activation in the lateral amygdala and the CA3 of hippocampus. In addition, propranolol treatment suppressed memory retrieval-induced CREB activation. These data indicate that US-retrieval activates more memory traces than CS-retrieval does, leading to memory reconsolidation of more CS-US associations.

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Section: Discussionmentioning

confidence: 99%

Unconditioned- and Conditioned- Stimuli Induce Differential Memory Reconsolidation and β-AR-Dependent CREB Activation

Bing

Zhu

Zhou

et al. 2017

Front. Neural Circuits

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“…Meanwhile, over 10 different clearing agents are in use (for review, see Refs. 9 , 11 , and 34 ). While a suitable and effective clearing method was essential for LSFM in the case of nonexpanded samples to achieve the required transparency, this aspect became irrelevant when combining it with expansion to LSFEM, because expanded samples were intrinsically transparent due to their high water content.…”

Section: Discussionmentioning

confidence: 99%

Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution

et al. 2019

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The goal of understanding the architecture of neural circuits at the synapse level with a brain-wide perspective has powered the interest in high-speed and large field-of-view volumetric imaging at subcellular resolution. Here, we developed a method combining tissue expansion and light-sheet fluorescence microscopy to allow extended volumetric super resolution high-speed imaging of large mouse brain samples. We demonstrate the capabilities of this method by performing two color fast volumetric super resolution imaging of mouse CA1 and dentate gyrus molecular-, granule cell-, and polymorphic layers. Our method enables an exact evaluation of granule cell and neurite morphology within the context of large cell ensembles spanning several orders of magnitude in resolution. We found that imaging a brain region of 1 mm 3 in super resolution using light-sheet fluorescence expansion microscopy is about 17-fold faster than imaging the same region by a current state-ofthe-art high-resolution confocal laser scanning microscope.

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“…The diffusion barrier properties caused by the lipid bilayer of cells are related to chemical penetration and light scattering properties of the lipid-water interface, making the tissue difficult to visualize [5,24,32]. Therefore, lipid extraction is critical for the optical transparency of tissue.…”

Section: Passive Claritymentioning

confidence: 99%

“…Biological tissue is a three-dimensional structure with complex detailed structures and vast molecular information, and how to acquire these data has always been a critical challenge [1][2][3][4][5][6][7][8]. This task has mainly relied on cutting the tissue into thin slices and provide cellular information at the two-dimensional level.…”

Section: Introductionmentioning

confidence: 99%