Optical biosensor test strip for the screening and direct determination of l-glutamate in food samples

Muslim, Noor Zuhartini Md; Ahmad, Musa; Heng, Lee Yook; Saad, Bahruddin

doi:10.1016/j.snb.2011.10.066

Cited by 33 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This compares favourably with a previously published value for MSG content in OXO cubes [7]. The quantity of glutamate recovered from the stock cube compares favourably with levels calculated with an optical biosensor, validated with HPLC [27] and utilising high performance thin layer chromatography [28].…”

Section: Application Of the Optimum Amperometric Biosensor (Mwcnt-chisupporting

confidence: 80%

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications

Hughes

Pemberton

Fielden

et al. 2015

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Disclaimer UWE has obtained warranties from all depositors as to their title in the material deposited and as to their right to deposit such material. UWE makes no representation or warranties of commercial utility, title, or fitness for a particular purpose or any other warranty, express or implied in respect of any material deposited. UWE makes no representation that the use of the materials will not infringe any patent, copyright, trademark or other property or proprietary rights.UWE accepts no liability for any infringement of intellectual property rights in any material deposited but will remove such material from public view pending investigation in the event of an allegation of any such infringement. The biosensor was fabricated by sequentially depositing the components on the surface of the transducer (MB-SPCE) in a layer-by-layer process, details of which are included in the paper. Each layer was optimized to construct the reagentless device.The biosensor was used in conjunction with amperometry in stirred solution using an applied potential of +0.1V (vs. Ag/AgCl). Optimum conditions for the analysis of glutamate were found to be: temperature, 35°C; phosphate buffer, pH 7 (0.75 mM, containing 0.05 M NaCl).The linear range of the reagentless biosensor was found to be 7.5 µM to 105 µM, and limit of detection was found to be 3 µM (based on n = 5, CV: 8.5% based on three times signal to noise) and the sensitivity was 0.39 nA/µM (± 0.025, coefficient of variation (CV) of 6.37%, n = 5). The response time of the biosensor was 20 -30 seconds.A food sample was analysed for monosodium glutamate (MSG). The endogenous content of MSG was 90.56 mg/g with a CV of 7.52%.The reagentless biosensor was also used to measure glutamate in serum. The endogenous concentration of glutamate was found to be 1.44 mM (n = 5), CV: 8.54%. The recovery of glutamate in fortified serum was 104% (n = 5), CV of 2.91%.

show abstract

Section: Application Of the Optimum Amperometric Biosensor (Mwcnt-chisupporting

confidence: 80%

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications

Hughes

Pemberton

Fielden

et al. 2015

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

show abstract

“…Various procedures were applied for the construction of biosensors such as enzyme entrapment into polymer matrix , physical adsorption by drop coating , crosslinking ,,,, layer‐by‐layer deposition , etc. The sensitivity of glutamate biosensors varied from 533 for biosensor operating at +35 °C based on GluOx covalently bound to polypyrrole‐nanoparticle‐polyaniline‐film to 0.52 μA cm −2 mmol L −1 for biosensor based on bacteria surface expressed glutamate dehydrogenase entrapped into the polyethylnediamine membrane . As seen, the characteristic analytical parameters of glutamate biosensor among the literature differs significantly as the biosensors are constructed employing different methods and they operate under divers conditions, such as potential, temperature, etc.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the determination of this compound is extremely important in both clinical and food samples. Various methods have been used to determine glutamate including high performance liquid chromatography (HPLC) ,, capillary electrophoresis , optical ,, and electrochemical techniques . Among the latter methods, a special attention is payed to electrochemical biosensors due to their sensitivity, selectivity and simple operation .…”

Section: Introductionmentioning

confidence: 99%

A Strategy to Employ Polymerised Riboflavin in the Development of Electrochemical Biosensors

et al. 2017

View full text Add to dashboard Cite

Electrochemically polymerised riboflavin (PRf) film was applied in development of glutamate and hypoxanthine biosensors using appropriate oxidases as recognition elements. PRf served as an enzyme immobilisation matrix composed of natural monomer and as a redox mediator. The PRf film was deposited on glassy carbon electrode (GCE) or on the laser‐designed microstructures of the chitosan‐graphene oxide (Chit‐GO) film deposited on glass slide, which served as a chip sensor. The redox polymer was stable and efficient mediator, however, Chit‐GO was placed on the top of PRf/GCE in order to increase the response signal. Such an approach allowed to extend the stability of glutamate and hypoxanthine biosensors to at least 1 month and 15 days, respectively. The chip microelectrode was a single use disposable biosensor due to insufficiently good Chit‐GO adhesion on the top of the cover‐glass. The glutamate microbiosensor had extremely high sensitivity of 16 μA cm−2 μmol L−1.

show abstract

“…H 2 O 2 has been detected by amperometric methods [16][17] or chromogenic reaction by using flow injection system [18] or test strip [19] .…”

mentioning

confidence: 99%

Colorimetric Determination of Monosodium Glutamate in Food Samples Using Colorimetric Determination of Monosodium Glutamate in Food Samples Using L-glutamate Oxidase-glutamate Oxidase

Alnokkari¹,

Ataie²,

Alasaf³

2013

Chinese Journal of Appplied Environmental Biology

View full text Add to dashboard Cite

A rapid, sensitive and simple chromogenic method was developed for quantitative determination of monosodium glutamate (MSG) in food samples. The method incorporated a glutamate oxidase and peroxidase. The librated H 2 O 2 from a glutamate sample as a result of enzymatic action was measured using 4-aminoantipyrine and phenol as a chromogenic reagent at 502 nm. Glutamate calibration curve was linear up to 125 mmol L -1 with a detection limit of 2 mmol L -1 . The results of analyzing L-glutamate in various food samples using the approach were compared to those of a standard procedure employing HPLC method. MSG concentration in different samples ranged from 0.93 to 4.9 g/kg. This method is relatively sensitive and specific, without the need of pre-treatment for sample. Fig 5, Tab 1, Ref 25

show abstract

Optical biosensor test strip for the screening and direct determination of l-glutamate in food samples

Cited by 33 publications

References 26 publications

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications

A Strategy to Employ Polymerised Riboflavin in the Development of Electrochemical Biosensors

Colorimetric Determination of Monosodium Glutamate in Food Samples Using Colorimetric Determination of Monosodium Glutamate in Food Samples Using L-glutamate Oxidase-glutamate Oxidase

Contact Info

Product

Resources

About