Optical assay using B-doped core–shell Fe@BC nanozyme for determination of alanine aminotransferase

Tian, Dazhi; Xiang, Wen; Wang, Hao; Jiang, Wentao; Li, Ting; Yang, Minghui

doi:10.1007/s00604-021-05056-w

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…ALT converts L-alanine and α-ketoglutarate into L-glutamate, and the oxidation of L-glutamate by glutamate oxidase produces H 2 O 2 , which further oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) to oxTMB by the POD-like activity of Fe@BC nanozyme, resulting in a blue-colored compound. The assay was measured in the dynamic range of 10 to 1000 U/L with a detection limit of 4 U/L [ 34 ]. Paper-based colorimetric assays for the detection of ALT and AST from various reports are summarized in Table 1 .…”

Section: Evaluation Of Hypothesis: Point-of-care Test For Diagnosis A...mentioning

confidence: 99%

Low-Cost Point-of-Care Monitoring of ALT and AST Is Promising for Faster Decision Making and Diagnosis of Acute Liver Injury

Chinnappan,

Mir,

Alsalameh

et al. 2023

Diagnostics

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Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) are important liver enzymes in clinical settings. Their levels are known to be elevated in individuals with underlying liver diseases and those consuming hepatotoxic drugs. Serum ALT and AST levels are crucial for diagnosing and assessing liver diseases. Serum ALT is considered the most reliable and specific candidate as a disease biomarker for liver diseases. ALT and AST levels are routinely analyzed in high-risk individuals for the bioanalysis of both liver function and complications associated with drug-induced liver injury. Typically, ALT and AST require blood sampling, serum separation, and testing. Traditional methods require expensive or sophisticated equipment and trained specialists, which is often time-consuming. Therefore, developing countries have limited or no access to these methods. To address the above issues, we hypothesize that low-cost biosensing methods (paper-based assays) can be applied to the analysis of ALT and AST levels in biological fluids. The paper-based biodetection technique can semi-quantitatively measure ALT and AST from capillary finger sticks, and it will pave the way for the development of an inexpensive and rapid alternative method for the early detection and diagnosis of liver diseases. This method is expected to significantly reduce the economic burden and aid routine clinical analysis in both developed and underdeveloped countries. The development of low-cost testing platforms and their diagnostic utility will be extremely beneficial in helping millions of patients with liver disorders.

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Section: Evaluation Of Hypothesis: Point-of-care Test For Diagnosis A...mentioning

confidence: 99%

Low-Cost Point-of-Care Monitoring of ALT and AST Is Promising for Faster Decision Making and Diagnosis of Acute Liver Injury

Chinnappan,

Mir,

Alsalameh

et al. 2023

Diagnostics

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“…The LOD of the sensor was found to be 4 U/L from the signal measured in the range of 10–1000 U/L. The results from this method were compared with the standard methods used in hospitals [ 41 ]. In another electrochemical sensing device, both ALT and AST were detected, in which the electrode was modified with pyruvate oxidase (POX) and oxaloacetate decarboxylase.…”

Section: Introductionmentioning

confidence: 99%

Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury

Chinnappan,

Mir,

Alsalameh

et al. 2023

Life

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Abnormal levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in human serum are the most sensitive indicator of hepatocellular damage. Because liver-related health problems are directly linked to elevated levels of ALT and AST, it is important to develop accurate and rapid methods to detect these enzymes for the early diagnosis of liver disease and prevention of long-term liver damage. Several analytical methods have been developed for the detection of ALT and AST. However, these methods are based on complex mechanisms and require bulky instruments and laboratories, making them unsuitable for point-of-care application or in-house testing. Lateral flow assay (LFA)-based biosensors, on the other hand, provide rapid, accurate, and reliable results, are easy to operate, and are affordable for low-income populations. However, due to the storage, stability, batch-to-batch variations, and error margins, antibody-based LFAs are considered unaffordable for field applications. In this hypothesis, we propose the selection of aptamers with high affinity and specificity for the liver biomarkers ALT and AST to build an efficient LFA device for point-of-care applications. Though the aptamer-based LFA would be semiquantitative for ALT and AST, it would be an inexpensive option for the early detection and diagnosis of liver disease. Aptamer-based LFA is anticipated to minimize the economic burden. It can also be used for routine liver function tests regardless of the economic situation in each country. By developing a low-cost testing platform, millions of patients suffering from liver disease can be saved.

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“…4,5 In addition to the stationary equipment in hospitals, there are many methods to detect liver function, including electrochemistry, fluorescence, and colorimetry methods. [6][7][8] Among them, electrochemistry methods have lower sensitivity and narrower detection range, fluorescence methods require expensive equipment and highly qualified operators, and colorimetry methods have greater influence on the test results from the environment. We used AST and ALT test strips for dry chemistry testing.…”

Section: Introductionmentioning

confidence: 99%

Portable dual-channel blood enzyme analyzer for point-of-care liver function detection

Sang,

Cheng,

et al. 2023

Analyst

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Optical assay using B-doped core–shell Fe@BC nanozyme for determination of alanine aminotransferase

Cited by 5 publications

References 25 publications

Low-Cost Point-of-Care Monitoring of ALT and AST Is Promising for Faster Decision Making and Diagnosis of Acute Liver Injury

Low-Cost Point-of-Care Monitoring of ALT and AST Is Promising for Faster Decision Making and Diagnosis of Acute Liver Injury

Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury

Portable dual-channel blood enzyme analyzer for point-of-care liver function detection

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