Optical Aptamer-Based Sensors for Detecting Plant Hormones

Feng, Silu; Chen, Changtian; Song, Chao; Ding, Xiaoke; Wang, Wei; Que, Long

doi:10.1109/jsen.2020.3041266

Cited by 14 publications

(14 citation statements)

References 36 publications

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“…In order to achieve label-free detection of pathogens on the chip, a portion of the inner FIP and BIP primers were immobilized on the sensors as shown in Figure A , so that the resulting LAMP products were bound to the sensor via immobilized inner primers, whereas all unbound DNA, primers, polymerase, dNTPs, and chemicals were removed by subsequent washing. To enable immobilization, FIP and BIP were designed to be modified by incorporating the amino-C-12 modifier to provide a 12-carbon spacer onto the 5′ terminus of each inner primer.…”

Section: Resultsmentioning

confidence: 99%

Integrated Sensing Chip for Ultrasensitive Label-Free Detection of the Products of Loop-Mediated Isothermal Amplification

et al. 2023

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Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique that has been widely used for the detection of pathogens in many organisms. Current LAMP-based sensors usually require the LAMP products to be labeled in order for them to be detected. Here, we present a novel label-free LAMP chip, which consists of a nanopore thin-film sensor embedded inside a LAMP reaction chamber. A fraction of LAMP primers is immobilized on the sensor surface, allowing the LAMP products to be synthesized and bound to the sensor surface via immobilized primers. After the LAMP reaction components are removed from the reaction chamber, the amplified LAMP products bound to the sensor surface give rise to significantly increased transducing signals, which can be measured by a portable optical spectrometer through an optical fiber probe. As a demonstration, we used the LAMP chip to detect the causal agent of late blight, Phytophthora infestans, which is one of the most devastating plant pathogens and poses a major threat to sustainable crop production worldwide. We show that this chip can detect as low as 1 fg/μL of P. infestans DNA in 30 min, which corresponds to an attomolar level of 1.6 × 10 −6 attomole/μL and is at least 10 times more sensitive than the currently available methods. This label-free sensing technology holds great promise to open up a new avenue for ultrasensitive, highly specific, rapid, and cost-effective point-of-care diagnostics of plant, animal, human, and foodborne pathogens.

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Section: Resultsmentioning

confidence: 99%

Integrated Sensing Chip for Ultrasensitive Label-Free Detection of the Products of Loop-Mediated Isothermal Amplification

et al. 2023

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“…Reusable biosensors can be generated using an aptamer as the receptor due to its reversible binding and temperature resistance. 72,73 The immobilization technique has a signicant effect on the stability of the biorecognition molecule. The adsorption technique is relatively simple but offers a lower lifespan than other methods.…”

Section: Quality Managementmentioning

confidence: 99%

“…The aptamer-based biosensor can be regenerated by heating it to 95 °C and washing it with a buffer. 73 Trace analysis of plant biomarkers is also crucial in predicting natural disasters and diseases. miRNA was found to be a good indicator for drought stress prediction.…”

Section: Wearable Sensorsmentioning

confidence: 99%

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Modern analytical and bioanalytical technologies and concepts for smart and precision farming

Tsong,

Khor

2023

Anal. Methods

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“…To rapidly and efficiently detect various plant hormones, Feng et al (Feng et al, 2020), developed a new chip equipped with aptamer-based sensors interfaced with a capillary microfluidics suitable for screening and detecting hormones in plant samples (Figure 10). Using this type of sensor, even if the hormones inside the sample are unknown, the sensor can screen or determine which type of plant hormones are in the samples.…”

Section: Reflectometric Interference Spectroscopymentioning

confidence: 99%

Advanced Nanoporous Anodic Alumina-Based Optical Sensors for Biomedical Applications

Feng

2021

Front. Nanotechnol.

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Close-packed hexagonal array nanopores are widely used both in research and industry. A self-ordered nanoporous structure makes anodic aluminum oxide (AAO) one of the most popular nanomaterials. This paper describes the main formation mechanisms for AAO, the AAO fabrication process, and optical sensor applications. The paper is focused on four types of AAO-based optical biosensor technology: surface-Enhanced Raman Scattering (SERS), surface Plasmon Resonance (SPR), reflectometric Interference Spectroscopy (RIfS), and photoluminescence Spectroscopy (PL). AAO-based optical biosensors feature very good selectivity, specificity, and reusability.

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Optical Aptamer-Based Sensors for Detecting Plant Hormones

Cited by 14 publications

References 36 publications

Integrated Sensing Chip for Ultrasensitive Label-Free Detection of the Products of Loop-Mediated Isothermal Amplification

Integrated Sensing Chip for Ultrasensitive Label-Free Detection of the Products of Loop-Mediated Isothermal Amplification

Modern analytical and bioanalytical technologies and concepts for smart and precision farming

Advanced Nanoporous Anodic Alumina-Based Optical Sensors for Biomedical Applications

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