Optical anisotropy composition of benign and malignant prostate tissues revealed by Mueller-matrix imaging

Abstract

A Mueller matrix imaging approach is employed to disclose the three-dimensional composition framework of optical anisotropy within cancerous biotissues. Visualized by the Mueller matrix technique spatial architecture of optical anisotropy of tissues is characterised by high-order statistical moments. Thus, quantitative analysis of the spatial distribution of optical anisotropy, such as linear and circular birefringence and dichroism, is revealed by using high-order statistical moments, enabling definitively discriminate prostate adenoma and carcinoma. The developed approach provides greater (>90%) accuracy of diagnostic achieved by using either the 3-rd or 4-th order statistical moments of the linear anisotropy parameters. Noticeable difference is observed between prostate adenoma and carcinoma tissue samples in terms of the extinction coefficient and the degree of depolarisation. Juxtaposition to other optical diagnostic modalities demonstrates the greater accuracy of the approach described herein, paving the way for its wider application in cancer diagnosis and tissue characterization.

3D polarization-interference holographic histology for wavelet-based differentiation of the polycrystalline component of biological tissues with different necrotic states. Forensic applications

“…Analytical processing of interference patterns was carried out using the digital Fourier transform FTðυ; νÞ: 27,28,35,36,38,44,45,49 E Q -T A R G E T ; t e m p : i n t r a l i n k -; e 0 2 7 ; 1 1 4 ; 5 0 2 FT x;y ðυ;…”

Nonlocal quantum differentiation between polarization objects using entanglement

“…Understanding the polarization response of an object of interest can reveal characteristic features in the sample's structure and/or behavior, e.g., the birefringence of crystals and biotissues, 1,2 polarization selectivity in reflection/transmission of periodic structures, [3][4][5] and chemical composition via detection of chiral proteins, molecules, or their assemblies. 6,7 The knowledge about the object under study that can be thus acquired is then widely employed for optical biomedical diagnostics, [8][9][10] the control of nonlinear phenomena, 11 fundamental studies, 12 technical characterization, 13,14 and remote sensing, 15 as well as for currently expanding quantum technologies in the fields of communication, computing, and metrology. 16,17 A comprehensive characterization in terms of polarization is enabled by the well-acknowledged techniques of classical optics-Stokes and Mueller polarimetry 18 -which are based on projective analysis of the polarization state or its change.…”

Mueller-matrix imaging polarimetry elevated by wavelet decomposition and polarization-singular processing for analysis of specific cancerous tissue pathology

“…The use of polarized light as an “instrumental probe” allows for assessing quantitatively optical anisotropy of the poly-crystalline structure of biological fluids and tissues 3 – 9 The polarization introscopy approach is well developed and known as Mueller-matrix (MM) microscopy in transmitted mode 10 – 15 In fact, MM microscopy is an example of a successful synthesis of instrumental imaging polarimetry, diverse theoretical modeling, and image processing, utilizing the regression model of optical anisotropy, 11 logarithmic MM decomposition, 12 – 15 Monte Carlo-based assessment of polarized light conversion, 14 and statistical analysis of MM images and optical anisotropy maps 11 , 15 .…”

“…It should be noted that the analyzed polarization introscopy methods 3 – 6 , 8 , 9 , 17 , 18 and MM 10 – 16 microscopy facilitate obtaining and visual analysis of the topological and coordinate structure of optical anisotropy maps of biological preparations. However, such analysis is somewhat subjective and does not provide a quantitative (objective) assessment of the severity or stages of pathology.…”