Optical activity and conformation of carbohydrates. II. Optical rotatory dispersion and circular dichroism studies on immunochemically reactive oligo- and polysaccharides containing amino sugars and their derivatives

Preparation and circular dichroism analysis of sodium hyaluronate oligosaccharides and chondroitin

“…These data are in fairly good agreement with previously reported spectra (Chakrabarti & Balazs, 1973;Buffington et al, 1977). The CD enhancement for NaHA is not directly related to the formation of glycosides per se, since methyl a-or /3-glycosides of the sugars exhibit properties nearly identical with those of the free sugars above 200 nm (Rabat et al, 1969;Morris et al, 1975;Coduti et al, 1977).…”

Purification, properties, and analysis of human asthmatic bronchial mucin

“…A distinctive characteristic of the circular dichroic spectra of both the intact mucin and the glycopeptide fraction was a negative trough at 210 mM for the glycopeptide fraction, similar to the results reported for milk oligosaccharides (Kabat et al, 1969). The intensity of the negative absorption observed after Pronase digestion of the mucin indicates that the spectrum is largely dependent on the carbohydrate moiety of the glycopeptide.…”

Structure and serological specificity of the K13-antigenic polysaccharide (K13 antigen) of urinary tract-infective Escherichia coli

“…Our measurements of the optical rotatory dispersion indicated a secondary structure of the K13 polysaccharide in which the carboxyl groups of KDO seem to play a role. Similar results were obtained with a polyneuraminic acid occurring in E. coli and N. meningitidis (12,13). In the K13 polysaccharide, the carboxyl groups of the KDO residues must be accessible, since they represent the major antigenic determinants of this antigen.…”