Optic nerve regeneration within artificial Schwann cell graft in the adult rat

Negishi, Hisanari; Dezawa, Mari; Oshitari, Toshiyuki; Adachi‐Usami, Emiko

doi:10.1016/s0361-9230(01)00534-2

Cited by 38 publications

(30 citation statements)

References 43 publications

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“…Al contrario que este tipo celular, las células de Schwann ejercen efectos positivos tanto en estadios tempranos como en adultos (38)(39)(40)(41) .…”

Section: Cultivos Bifásicosunclassified

“…La segunda compresión fue de 4 minutos a 140⁰C y 100 bares y la tercera compresión tuvo lugar a 140⁰C y 200 bares durante 20 segundos. Entre cada compresión se enfriaban las placas de la prensa hasta 60⁰C y con una presión de entre [40][41][42][43][44][45][46][47][48][49][50] bares. Además, se deben precalentar las placas durante 1 minuto antes de la segunda y la tercera compresión.…”

Section: Elaboración Del "Scaffold" 3d De Peaunclassified

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Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Gamboa-MartÍnez

Ribelles

Ferrer

2011

Journal of Bioactive and Compatible Polymers

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A hybrid scaffold was obtained by the deposition of a thin network of submicron fibrin fibrils on the microporous walls of a macroporous poly(L-lactide) (PLLA) three-dimensional structure. The fibrin coating is homogeneous across the entire substrate and allowed the pore structure remain open in the hybrid scaffold. The elastic modulus of the hybrid scaffold (0.65 MPa) was increased up to twofold compared to the pure PLLA scaffold (0.29 MPa). Mouse pre-osteoblastic cells, MC3T3, were seeded on both pure PLLA and hybrid scaffolds, and cultured for 3, 6, and 24 h. The coating enhanced the cell colonization and proliferation and provided a more homogeneous distribution of cells within the scaffolds. In addition, the coating improved the scaffold adhesion properties by supplying new binding sites to the cells that modify the transmembrane receptors involved in initial cell adhesion mechanism. The expression of the β3 integrin was observed in cells cultured on fibrin-coated scaffolds instead of the α5 integrin, which was expressed in the uncoated scaffold. These hybrid PLLA/fibrin scaffolds have cell culture features suitable to promote early tissue regeneration.

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“…Al contrario que este tipo celular, las células de Schwann ejercen efectos positivos tanto en estadios tempranos como en adultos (38)(39)(40)(41) .…”

Section: Cultivos Bifásicosunclassified

Section: Elaboración Del "Scaffold" 3d De Peaunclassified

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Gamboa-MartÍnez

Ribelles

Ferrer

2011

Journal of Bioactive and Compatible Polymers

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“…Single BDNF intravitreal injections confer no protection in experimental glaucoma36, nor in experimental retinal detachment37, but BDNF injection and virally-mediated overexpression of BDNF slow RGC death38,39,40 and increase RGC regeneration41 after optic nerve transection. Repeated BDNF injections alone or by injection combined with additional measures prevent some experimental glaucoma injury42,43.…”

Section: Introductionmentioning

confidence: 99%

Effect of CNTF on Retinal Ganglion Cell Survival in Experimental Glaucoma

Pease

Zack

Berlinicke

et al. 2009

Invest. Ophthalmol. Vis. Sci.

208

137

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Purpose To assess the neuroprotective effect of virally-mediated over-expression of ciliary derived neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) in experimental rat glaucoma. Methods Laser-induced glaucoma was produced in one eye of 224 Wistar rats after injection of adeno-associated viral vectors (type 2) containing either CNTF, BDNF or both, using saline injected eyes and uninjected glaucoma eyes as controls. IOP was measured with the TonoLab and semi-automated optic nerve axon counts were performed by masked observers. IOP exposure over time was adjusted in multivariate regression analysis to calculate the effect of CNTF and BDNF. Results By multivariate regression, CNTF had a significant protective effect, with 15% less RGC axon death (p < 0.01). Both combined CNTF—BDNF and BDNF over-expression alone had no statistically significant improvement in RGC axon survival. By Western blot, there was a quantitative increase in CNTF and BDNF expression in retinas exposed to single viral vectors carrying each gene, but no increase with sequential injection of both vectors. Conclusion These data confirm that CNTF can exert a protective effect in experimental glaucoma. The reason for a lack of observed effect with the BDNF overexpression groups is unclear, but may be a function of the level of neurotrophin expression achieved.

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“…They showed that neurotrophin receptors (tropomyosin receptor kinase [Trk]-A and Trk-B) were detected in nerve fibers in both retina and graft. 28 On the axonal regeneration by PN transplantation in cats, a combined injection of BDNF, CNTF, and forskolin resulted in a 3.4-fold increase of beta-RGCs with regenerating axons. Watanabe et al suggested that the increase of regenerating beta-RGCs was mainly due to the enhanced effect of neurotrophic factors on their survival, and possibly to a change of RGC properties by cAMP to facilitate their axonal regeneration.…”

Section: Neurotrophic Factorsmentioning

confidence: 99%

Restoration of optic neuropathy

You¹,

Wu²,

Kuang³

et al. 2017

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Optic neuropathy refers to disorders involving the optic nerve (ON). Any damage to ON or ON-deriving neurons, the retinal ganglion cells (RGCs), may lead to the breakdown of the optical signal transmission from the eye to the brain, thus resulting in a partial or complete vision loss. The causes of optic neuropathy include trauma, ischemia, inflammation, compression, infiltration, and mitochondrial damages. ON injuries include primary and secondary injuries. During these injury phases, various factors orchestrate injured axons to die back and become unable to regenerate, and these factors could be divided into two categories: extrinsic and intrinsic. Extrinsic inhibitory factors refer to the environmental conditions that influence the regeneration of injured axons. The presence of myelin inhibitors and glial scar, lack of neurotrophic factors, and inflammation mediated by injury are regarded as these extrinsic factors. Extrinsic factors need to trigger the intracellular signals to exert inhibitory effect. Proper regulation of these intracellular signals has been shown to be beneficial to ON regeneration. Intrinsic factors of RGCs are the pivotal reasons that inhibit ON regeneration and are closely linked with extrinsic factors. Intracellular cyclic adenosine monophosphate (cAMP) and calcium levels affect axon guidance and growth cone response to guidance molecules. Many genes, such as Bcl-2, PTEN, and mTOR, are crucial in cell proliferation, axon guidance, and growth during development, and play important roles in the regeneration and extension of RGC axons. With transgenic mice and related gene regulations, robust regeneration of RGC axons has been observed after ON injury in laboratories. Although various means of experimental treatments such as cell transplantation and gene therapy have achieved significant progress in neuronal survival, axonal regeneration, and restoration of the visual function after ON injury, many unresolved scientific problems still exist for their clinical applications. Therefore, we still need to overcome hurdles before developing effective therapy to treat optic neuropathy diseases in patients.

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Optic nerve regeneration within artificial Schwann cell graft in the adult rat

Cited by 38 publications

References 43 publications

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Effect of CNTF on Retinal Ganglion Cell Survival in Experimental Glaucoma

Restoration of optic neuropathy

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