OPT3 Is a Phloem-Specific Iron Transporter That Is Essential for Systemic Iron Signaling and Redistribution of Iron and Cadmium in<i>Arabidopsis</i>

Zhai, Zhiyang; Gayomba, Sheena R.; Jung, Ha-il; Vimalakumari, Nanditha K.; Piñeros, Miguel A.; Craft, Eric; Rutzke, Michael; Danku, John; Lahner, Brett; Punshon, Tracy; Guerinot, Mary Lou; Salt, David E.; Kochian, Leon V.; Vatamaniuk, Olena K.

doi:10.1105/tpc.114.123737

Cited by 228 publications

(266 citation statements)

References 73 publications

(111 reference statements)

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“…This suggests that the phloem Fe compound acting as a repressive signal could be a peptide (probably a Fe-peptide) transported by OPT3 [82,87]. However, recently it has been published that OPT3 could transport Fe ions into the phloem [89]. Even if these results are confirmed, it would not be ruled out that the Fe ions, once inside the phloem, could be bound to organic compounds to be transported.…”

Section: Hormones and Signaling Substances In The Regulation Of Fe Ancontrasting

confidence: 39%

“…Even if these results are confirmed, it would not be ruled out that the Fe ions, once inside the phloem, could be bound to organic compounds to be transported. Taken together, the results obtained with the frd3 and opt3-2 mutants suggest that Fe availability, represented by a phloem Fe-related signal, could participate in the regulation of Fe acquisition genes in roots along with ethylene and other hormones [82,89,90].…”

Section: Hormones and Signaling Substances In The Regulation Of Fe Anmentioning

confidence: 62%

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Similarities and Differences in the Acquisition of Fe and P by Dicot Plants

et al. 2018

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This review deals with two essential plant mineral nutrients, iron (Fe) and phosphorus (P); the acquisition of both has important environmental and economic implications. Both elements are abundant in soils but are scarcely available to plants. To prevent deficiency, dicot plants develop physiological and morphological responses in their roots to specifically acquire Fe or P. Hormones and signalling substances, like ethylene, auxin and nitric oxide (NO), are involved in the activation of nutrient-deficiency responses. The existence of common inducers suggests that they must act in conjunction with nutrient-specific signals in order to develop nutrient-specific deficiency responses. There is evidence suggesting that P-or Fe-related phloem signals could interact with ethylene and NO to confer specificity to the responses to Fe-or P-deficiency, avoiding their induction when ethylene and NO increase due to other nutrient deficiency or stress. The mechanisms responsible for such interaction are not clearly determined, and thus, the regulatory networks that allow or prevent cross talk between P and Fe deficiency responses remain obscure. Here, fragmented information is drawn together to provide a clearer overview of the mechanisms and molecular players involved in the regulation of the responses to Fe or P deficiency and their interactions.

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Section: Hormones and Signaling Substances In The Regulation Of Fe Ancontrasting

confidence: 39%

Section: Hormones and Signaling Substances In The Regulation Of Fe Anmentioning

confidence: 62%

Similarities and Differences in the Acquisition of Fe and P by Dicot Plants

et al. 2018

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“…Several reports have indicated the importance of proper loading of metal ions into seeds for successful plant reproduction. The role of YSL (AtYSL1 and AtYSL3) and the oligopeptide transporter (AtOPT3) protein family in iron translocation to developing embryo and into the seed coat is critical for reproductive growth (Le Jean et al, 2005;Stacey et al, 2008;Chu et al, 2010;Mendoza-Cózatl et al, 2014;Zhai et al, 2014). Additionally, the role of Arabidopsis thaliana FERRIC REDICTASE DEFECTIVE3 (AtFRD3) in iron acquisition in reproductive tissues such as pollen grain and embryos suggests a complex mechanism of iron nutrition during embryogenesis (Roschzttardtz et al, 2011b).…”

Section: Discussionmentioning

confidence: 99%

Iron-Binding E3 Ligase Mediates Iron Response in Plants by Targeting Basic Helix-Loop-Helix Transcription Factors

et al. 2014

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Iron uptake and metabolism are tightly regulated in both plants and animals. In Arabidopsis (Arabidopsis thaliana), BRUTUS (BTS), which contains three hemerythrin (HHE) domains and a Really Interesting New Gene (RING) domain, interacts with basic helix-loop-helix transcription factors that are capable of forming heterodimers with POPEYE (PYE), a positive regulator of the iron deficiency response. BTS has been shown to have E3 ligase capacity and to play a role in root growth, rhizosphere acidification, and iron reductase activity in response to iron deprivation. To further characterize the function of this protein, we examined the expression pattern of recombinant ProBTS::b-GLUCURONIDASE and found that it is expressed in developing embryos and other reproductive tissues, corresponding with its apparent role in reproductive growth and development. Our findings also indicate that the interactions between BTS and PYE-like (PYEL) basic helix-loop-helix transcription factors occur within the nucleus and are dependent on the presence of the RING domain. We provide evidence that BTS facilitates 26S proteasome-mediated degradation of PYEL proteins in the absence of iron. We also determined that, upon binding iron at the HHE domains, BTS is destabilized and that this destabilization relies on specific residues within the HHE domains. This study reveals an important and unique mechanism for plant iron homeostasis whereby an E3 ubiquitin ligase may posttranslationally control components of the transcriptional regulatory network involved in the iron deficiency response.

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“…Analysis of our RNA-seq data revealed that the expression of several genes from (A) Seeds of the indicated plant lines were germinated and grown in soil, and fertilized with the N-P-K fertilizer and a standard nutrient solution (Zhai et al, 2014). Note the retarded growth of the citf1 spl7 double mutant seedling (inside a white square) that eventually died.…”

Section: Citf1 and Spl7 Are Required For Cu Delivery To Flowersmentioning

confidence: 99%

“…Different Arabidopsis plant lines were grown either on solid 0.53 Murashige and Skoog (MS) medium (pH 5.7) (Murashige and Skoog, 1962) or hydroponically (Zhai et al, 2014). For growing plants on solid medium, seeds were surface-sterilized as described (Zhai et al, 2014).…”

Section: Growth Conditions and Experimental Treatmentsmentioning

confidence: 99%

Arabidopsis Pollen Fertility Requires the Transcription Factors CITF1 and SPL7 That Regulate Copper Delivery to Anthers and Jasmonic Acid Synthesis

et al. 2017

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A deficiency of the micronutrient copper (Cu) leads to infertility and grain/seed yield reduction in plants. How Cu affects fertility, which reproductive structures require Cu, and which transcriptional networks coordinate Cu delivery to reproductive organs is poorly understood. Using RNA-seq analysis, we showed that the expression of a gene encoding a novel transcription factor, CITF1 (Cu-DEFICIENCY INDUCED TRANSCRIPTION FACTOR1), was strongly upregulated in Arabidopsis thaliana flowers subjected to Cu deficiency. We demonstrated that CITF1 regulates Cu uptake into roots and delivery to flowers and is required for normal plant growth under Cu deficiency. CITF1 acts together with a master regulator of copper homeostasis, SPL7 (SQUAMOSA PROMOTER BINDING PROTEIN LIKE7), and the function of both is required for Cu delivery to anthers and pollen fertility. We also found that Cu deficiency upregulates the expression of jasmonic acid (JA) biosynthetic genes in flowers and increases endogenous JA accumulation in leaves. These effects are controlled in part by CITF1 and SPL7. Finally, we show that JA regulates CITF1 expression and that the JA biosynthetic mutant lacking the CITF1-and SPL7-regulated genes, LOX3 and LOX4, is sensitive to Cu deficiency. Together, our data show that CITF1 and SPL7 regulate Cu uptake and delivery to anthers, thereby influencing fertility, and highlight the relationship between Cu homeostasis, CITF1, SPL7, and the JA metabolic pathway.

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OPT3 Is a Phloem-Specific Iron Transporter That Is Essential for Systemic Iron Signaling and Redistribution of Iron and Cadmium inArabidopsis

Cited by 228 publications

References 73 publications

Similarities and Differences in the Acquisition of Fe and P by Dicot Plants

Similarities and Differences in the Acquisition of Fe and P by Dicot Plants

Iron-Binding E3 Ligase Mediates Iron Response in Plants by Targeting Basic Helix-Loop-Helix Transcription Factors

Arabidopsis Pollen Fertility Requires the Transcription Factors CITF1 and SPL7 That Regulate Copper Delivery to Anthers and Jasmonic Acid Synthesis

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