Opsonizing and bactericidal effects of normal human serum on nontypable Haemophilus influenzae

Musher, Daniel M.; Hague-Park, Mickey; Baughn, Robert E.; Wallace, Richard J.; Cowley, Benjamin D.

doi:10.1128/iai.39.1.297-304.1983

Cited by 65 publications

(25 citation statements)

References 26 publications

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“…A number of previous studies have established clearly that serum is bactericidal for NTHi [15,16]. The effectiveness of serum killing has not been well characterized in subjects with chronic bronchial infection with NTHi.…”

Section: Discussionmentioning

confidence: 99%

Systemic humoral immunity to non-typeable Haemophilus influenzae

King

Ngui

Gunawardena

et al. 2008

Clinical and Experimental Immunology

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SummaryNon-typeable Haemophilus influenzae (NTHi) is a major cause of respiratory but rarely systemic infection. The host defence to this bacterium has not been well defined in patients with chronic airway infection. The aim of this study was to assess the effect of humoral immunity in host defence to NTHi. Responses were measured in control and bronchiectasis subjects who had recurrent bronchial infection. Antibody and complement-mediated killing was assessed by incubating NTHi with serum and the role of the membraneattack complex and classical/alternate pathways of complement activation measured. The effect of one strain to induce protective immunity against other strains was assessed. The effect of antibody on granulocyte intracellular killing of NTHi was also measured. The results showed that both healthy control subjects and bronchiectasis patients all had detectable antibody to NTHi of similar titre. Both groups demonstrated effective antibody/ complement-mediated killing of different strains of NTHi. This killing was mediated through the membrane-attack complex and the classical pathway of complement activation. Immunization of rabbits with one strain of NTHi resulted in protection from other strains in vitro. Antibody activated granulocytes to kill intracellular bacteria. These findings may explain why NTHi rarely causes systemic disease in patients with chronic respiratory mucosal infection and emphasize the potential importance of cellular immunity against this bacterium.

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Section: Discussionmentioning

confidence: 99%

Systemic humoral immunity to non-typeable Haemophilus influenzae

King

Ngui

Gunawardena

et al. 2008

Clinical and Experimental Immunology

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“…Antibody responses to antigens that arise as a result of either infection or immunization play a role in facilitating bacterial clearance. This is achieved by a number of mechanisms including bactericidal activity and opsonophagocytosis by polymorphonuclear leukocytes (26); however, in mucosal defenses the role of IgA in inhibiting microbial adherence is a significant first line defense against infection (22). With respect to antibody titer and specificity, immunization with the recombinant OMP26 that included the leader sequence induced higher titers of both IgG and IgA that recognized both recombinant proteins equivalently.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Gene Encoding a 26-Kilodalton Protein (OMP26) from Nontypeable Haemophilus influenzae and Immune Responses to the Recombinant Protein

et al. 1999

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A 26-kDa protein (OMP26) isolated and purified from nontypeable Haemophilus influenzae (NTHI) strain 289 has been shown to enhance clearance of infection following pulmonary challenge with NTHI in rats. DNA sequence analysis revealed that it was 99% identical to a gene encoding a cell envelope protein of the H. influenzae Rd strain (TIGR accession no. HI0916). The deduced amino acid sequence revealed a hydrophilic polypeptide rich in basic amino acids. Restriction fragment length polymorphism analysis suggested that the OMP26 gene was relatively conserved among isolates of NTHI. Analysis of the deduced amino acid sequence of the OMP26 gene from 20 different isolates showed that similarity with NTHI-289 ranged from 96.5% (1 isolate) to 99.5% (14 isolates). Two recombinant forms of OMP26, a full length 28-kDa protein (equivalent to preprotein) and a 26-kDa protein lacking a 23-amino-acid leader peptide (equivalent to processed protein), were assessed in immunization studies for the ability to induce an immune response that would be as effective as the native protein in enhancing the clearance of NTHI following pulmonary challenge in rats. Immunization with the recombinant protein that included the leader peptide was more effective in enhancing pulmonary clearance, and it induced a better cell-mediated response and higher titers of systemic and mucosal antibody. This study has characterized a 26-kDa protein from NTHI that shows significant potential as a vaccine candidate.

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“…Since the presence of bactericidal antibodies in serum is related to protection from infection due to H. influenzae type b (9) and encapsulated meningococci (11), there has been interest in studying bactericidal antibody to nontypeable H. influenzae in human serum (4,7,10,13,23,24). Shurin et al (29) have provided indirect evidence that the presence of bactericidal antibody is correlated with protection from otitis media caused by the bacterium.…”

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confidence: 99%