Opposite regulation of the HPV 20-URR and HPV 27-URR promoters by ultraviolet irradiation and cytokines

Ruhland, Astrid; Villiers, Ethel Michele De

doi:10.1002/1097-0215(200002)9999:9999<::aid-ijc1129>3.0.co;2-b

Cited by 35 publications

(16 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Future research could involve growing HPV-76 and HPV types from the b2 and c HPV group in raft culture (Bell et al, 1983;Regnier et al, 1981), so the mechanism of action of these HPV types in skin tissues can be investigated. UV radiation damages DNA by inducing mutations and impairing apoptosis, leading to cancer formation (Ruhland & de Villiers, 2001). It has also been found to have suppressing effects on the immune system through skinmediated responses (Kripke, 1988;Morison, 1989;Noonan & De Fabo, 1992).…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus type spectrum in normal skin of individuals with or without a history of frequent sun exposure

Chen

McMillan

Antonsson

2008

Journal of General Virology

View full text Add to dashboard Cite

Cutaneous human papillomavirus (HPV) has been widely detected in healthy skin. Previous studies have found that UV radiation can activate several HPV types, and a possible role for cutaneous HPV in the development of non-melanoma skin cancer has been suggested. This study investigated the prevalence and type-spectrum of cutaneous HPV in relation to UV radiation by studying forehead skin swab samples from 50 healthy males frequently exposed to the sun and 50 healthy males who were not frequently exposed to the sun. A questionnaire including ethnic background of the participants, history of cancers and a self-assessment of sun-exposure was also conducted and analysed. PCR with the FAP primer pair was carried out to detect HPV DNA in samples. HPV prevalence was higher in individuals who spent more time outdoors and in individuals with a history of skin cancers (P50.044 and P50.04, respectively). Furthermore, individuals wearing sunglasses as a means of sun protection had a lower prevalence of HPV (P50.018). Interestingly, HPV-76 was only detected in the group without frequent sun-exposure (P50.001). These results suggest that increased UV radiation exposure may be a factor leading to a difference in prevalence of cutaneous HPV types. INTRODUCTIONHuman papillomaviruses (HPVs) infect cutaneous and mucosal epithelia and are recognized as the causative agents of warts. It has recently become apparent that clinically normal skin harbours many different HPV types, and many of these HPV types have not been described previously (Antonsson et al., 2000(Antonsson et al., , 2003a Astori et al., 1998). Furthermore, it has been shown that asymptomatic HPV infections are acquired very early in infancy with a broad spectrum of HPV types (Antonsson et al., 2003b), and that the prevalence of HPV DNA in healthy skin increases with age (Antonsson et al., 2000). Moreover, cutaneous HPV infections on healthy skin have often been found to persist over time (Hazard et al., 2006). Many studies have also suggested a role for HPV in the carcinogenesis of non-melanoma skin cancer (NMSC). Squamous cell carcinoma (SCC) can metastasize, and is known as the unfavourable form of NMSC compared with basal cell carcinoma (BCC). Interestingly, BCCs are found approximately three times more frequently in immunocompetent individuals; however, in immunosuppressed organ-transplant recipients, SCCs are more prevalent (Pfister, 2003). It has been reported that more HPV types have been found in actinic keratosis and SCC skin lesions than in normal skin (Alotaibi et al., 2006), and a greater variety of HPV types have also been found in immunosuppressed individuals than in immunocompetent individuals (Harwood et al., 2004). Both SCC and BCC develop mostly on sun-exposed sites (Frost & Green, 1994), and some specific types of HPV, including HPV-5, HPV-8, HPV-20 and HPV-77, have been shown to be activated by UV radiation (Akgül et al., 2005;Massimi et al., 2008;Michel et al., 2006;Purdie et al., 1999;Storey et al., 1998). Evidence of UV-induced E6/E7 oncogenici...

show abstract

Section: Discussionmentioning

confidence: 99%

Human papillomavirus type spectrum in normal skin of individuals with or without a history of frequent sun exposure

Chen

McMillan

Antonsson

2008

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The mechanistic role that individual viral proteins of the mucosal papillomaviruses play in the malignant conversion of genital keratinocytes has been described in detail (zur Hausen, 1996;Fehrmann and Laimins, 2003). In contrast, data on the mechanisms by which cutaneous papillomaviruses may function to influence cell proliferation and malignant progression (Jackson and Storey, 2000;Caldeira et al, 2003) or vice versa, the way by which external and cellular factors regulate the cutaneous papillomavirus activity (de Villiers et al, 1999;Ruhland and de Villiers, 2001) have been fractional.…”

Section: Discussionmentioning

confidence: 99%

“…H1299 cells were transfected with either of the pUC18-RSVcJun, pUC18-RSV-JunB, pUC18-RSV-JunD, pRc/CMV-p53 R248W or the pcDNA3.1( þ ) plasmid containing the HA-tagged TAp63a, pcDNA3.1( þ )-TAp63aDSAMDTI, pcDNA3.1( þ )-TAp63aDTI and pcDNA3.1( þ ), respectively. Transfections (4 mg per construct per 1 Â 10 6 cells) were performed using LipofectAmine (Gibco BRL, Paisley, Scotland) as previously described (Ruhland and de Villiers, 2001). All transfections were carried out with a GFP expressing plasmid in addition to control for equal levels of transfection efficiency.…”

Section: Deletion Constructsmentioning

confidence: 99%

“…Â 10 min). These protein extracts (10 mg) were separated by SDS-PAGE on 10% polyacrylamide gels, electro-blotted onto nitrocellulose membranes (Schleicher & Schuell, Dassel, Germany) and treated as previously described (Ruhland and de Villiers, 2001). The membranes were blocked with 5% non-fat dried milk in TBST (1 Â TBS, 0.1% Tween-20).…”

Section: Western Immunoblot Analysismentioning

confidence: 99%

“…The differential activity of the Jun-proteins plays a role in human papillomavirus-linked carcinogenesis in the genital tract (reviewed in zur Hausen, 1996) and increasing data are shedding light on the role of these proteins in the life cycle of mainly the mucosal HPV types in differentiating keratinocytes (Longworth and Laimins, 2004). The HPV types associated with cutaneous lesions react diversely in response to external or cellular factors (de Villiers et al, 1999;Ruhland and de Villiers, 2001). The expression of c-Jun and JunB is enhanced under the expression of bovine papillomavirus in mouse dermal fibroblasts leading to the formation of fibromatosis (Bossy-Wetzel et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

TAp63α indirectly regulates a cutaneous HPV promoter through complex formation with Jun family members

et al. 2006

View full text Add to dashboard Cite

Papillomavirus Infections and Cancerogenesis of Squamous Cell and Basal Cell Carcinomas

Bens

Medical Intelligence Unit

View full text Add to dashboard Cite

Opposite regulation of the HPV 20-URR and HPV 27-URR promoters by ultraviolet irradiation and cytokines

Cited by 35 publications

References 40 publications

Human papillomavirus type spectrum in normal skin of individuals with or without a history of frequent sun exposure

Human papillomavirus type spectrum in normal skin of individuals with or without a history of frequent sun exposure

TAp63α indirectly regulates a cutaneous HPV promoter through complex formation with Jun family members

Papillomavirus Infections and Cancerogenesis of Squamous Cell and Basal Cell Carcinomas

Contact Info

Product

Resources

About