Opposite extremes in ethylene/nitric oxide ratio induce cell death in suspension culture and root apices of tomato exposed to salt stress

Poór, Péter; Borbély, Péter; Kovács, Judit; Papp, Anita; Szepesi, Ágnes; Takács, Zoltan; Tari, Irma

doi:10.1556/abiol.65.2014.4.7

Cited by 16 publications

(19 citation statements)

References 43 publications

(49 reference statements)

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“…Disintegration of cell membranes was determined by the measurement of electrolyte leakage [18]. Tissue segments of the same size were collected on a filter paper and washed twice with 50 ml of double distilled water and then 0.5 g of fresh tissues was incubated in 20 ml of double distilled water at 25 °C for 2 hours.…”

Section: Electrolyte Leakage Measurementmentioning

confidence: 99%

Salicylic acid induced cysteine protease activity during programmed cell death in tomato plants

Kovács¹,

Poór²,

Szepesi³

et al. 2016

Acta Biologica Hungarica

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The hypersensitive response (HR), a type of programmed cell death (PCD) during biotic stress is mediated by salicylic acid (SA). The aim of this work was to reveal the role of proteolysis and cysteine proteases in the execution of PCD in response of SA. Tomato plants were treated with sublethal (0.1 mM) and lethal (1 mM) SA concentrations through the root system. Treatment with 1 mM SA increased the electrolyte leakage and proteolytic activity and reduced the total protein content of roots after 6 h, while the proteolytic activity did not change in the leaves and in plants exposed to 0.1 mM SA. The expression of the papain-type cysteine protease SlCYP1, the vacuolar processing enzyme SlVPE1 and the tomato metacaspase SlMCA1 was induced within the first three hours in the leaves and after 0.5 h in the roots in the presence of 1 mM SA but the transcript levels did not increase significantly at sublethal SA. The Bax inhibitor-1 (SlBI-1), an antiapoptotic gene was over-expressed in the roots after SA treatments and it proved to be transient in the presence of sublethal SA. Protease inhibitors, SlPI2 and SlLTC were upregulated in the roots by sublethal SA but their expression remained low at 1 mM SA concentration. It is concluded that in contrast to leaves the SA-induced PCD is associated with increased proteolytic activity in the root tissues resulting from a fast up-regulation of specific cysteine proteases and down-regulation of protease inhibitors.

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Section: Electrolyte Leakage Measurementmentioning

confidence: 99%

Salicylic acid induced cysteine protease activity during programmed cell death in tomato plants

Kovács¹,

Poór²,

Szepesi³

et al. 2016

Acta Biologica Hungarica

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“…and H 2 O 2 ) and nitrogen (NO and ONOO -) forms in the root apices of wild type and ethylene receptor mutants and to reveal correlations between their accumulation and cell viability. We are also interested in how various forms of reactive oxygen and nitrogen affect the acclimation to salt stress or the initiation of PCD in Nr mutants exposed to salinity stress which had been found to be sublethal and lethal stress in wild type tomato roots (Poór et al 2014).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Flow rates were 35 mL min -1 for He, 30 mL min -1 for H 2 and 300 mL min -1 for air. The oven, injector and detector temperatures were 300, 120 and 100 o C, respectively (Poór et al, 2014). A set of ethylene standards was used to calculate the amount of ethylene generated by the root segments.…”

Section: Measurement Of Ethylene Productionmentioning

confidence: 99%

“…Cell death was determined with the measurement of electrolyte leakage (EL) by the method of Poór et al (2014). One g of root segments was transferred to 15 mL double distilled water.…”

Section: Cell Death Determinationmentioning

confidence: 99%

“…Potassium and sodium contents of the root cells were determined by AAS (Hitachi Z-8200, Tokyo, Japan) (Poór et al, 2014).…”

Section: Determination Of Macroelement Contentsmentioning

confidence: 99%

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Salt stress-induced production of reactive oxygen- and nitrogen species and cell death in the ethylene receptor mutant Never ripe and wild type tomato roots

Poór

Kovács

Borbély

et al. 2015

Plant Physiology and Biochemistry

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The salt stress triggered by sublethal, 100 mM and lethal, 250 mM NaCl induced ethylene production as well as rapid accumulation of superoxide radical and H2O2 in the root tips of tomato (Solanum lycopersicum cv. Ailsa Craig) wild type and ethylene receptor mutant, Never ripe (Nr/Nr) plants. In the wild type plants superoxide accumulation confined to lethal salt concentration while H2O2 accumulated more efficiently under sublethal salt stress. However, in Nr roots the superoxide production was higher and unexpectedly, H2O2 level was lower than in the wild type under sublethal salt stress. Nitric oxide production increased significantly under sublethal and lethal salt stress in both genotypes especially in mutant plants, while peroxynitrite accumulated significantly under lethal salt stress. Thus, the nitro-oxidative stress may be stronger in Nr roots, which leads to the programmed death of tissues, characterized by the DNA and protein degradation and loss of cell viability under moderate salt stress. In Nr mutants the cell death was induced in the absence of ethylene perception. Although wild type roots could maintain their potassium content under moderate salt stress, K(+) level significantly declined leading to small K(+)/Na(+) ratio in Nr roots. Thus Nr mutants were more sensitive to salt stress than the wild type and the viability of root cells decreased significantly under moderate salt stress. These changes can be attributed to a stronger ionic stress due to the K(+) loss from the root tissues.

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Ethylene and Nitric Oxide Under Salt Stress

Iqbal

Saffeullah

Umar

2022

Ethylene in Plant Biology

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Opposite extremes in ethylene/nitric oxide ratio induce cell death in suspension culture and root apices of tomato exposed to salt stress

Cited by 16 publications

References 43 publications

Salicylic acid induced cysteine protease activity during programmed cell death in tomato plants

Salicylic acid induced cysteine protease activity during programmed cell death in tomato plants

Salt stress-induced production of reactive oxygen- and nitrogen species and cell death in the ethylene receptor mutant Never ripe and wild type tomato roots

Ethylene and Nitric Oxide Under Salt Stress

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