Opposite effects of moderate heat stress and hyperthermia on cholinergic system of soil nematodes Caenorhabditis elegans and Caenorhabditis briggsae

Kalinnikova, T. B.; Колсанова, Р Р; Belova, E. B.; Шагидуллин, Р Р; Gainutdinov, M. Kh.

doi:10.1016/j.jtherbio.2016.05.007

Cited by 9 publications

(2 citation statements)

References 69 publications

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“…It is known that levamisole disturbs nematodes swimming due to binding with nAChRs in neural circuit regulating locomotion or in body muscles. In both cases the hypercontraction of locomotory muscles occurs leading to failure in coordination of movements during swimming, and under more continuous impact -to nematodes paralysis [9][10]. Results of our experiments give the evidence that A. sativum juice contains components which increase nAChRs sensitivity to levamisole action.…”

Section: Resultsmentioning

confidence: 64%

Soil nematodeCaenorhabditis elegansMaupas as a convenient model organism to study anthelmintic activity of plant extracts

et al. 2020

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The article deals with the study of the possibility to use free-living soil nematode C. elegans in researches of anthelmintic activity of plant raw material. A. sativum juice and aqueous extract of T. vulgare flowers were used in experiments. It is shown that both A. sativum juice and tansy flowers extract caused dose-dependent disturbances of C. elegans swimming motor program. These disturbances are similar with disturbances caused by agonist of cholinoreceptors levamisole. Therefore, the target of A. sativum juice and T. vulgare extract action is C. elegans cholinergic system. The mechanism of toxic action of A. sativum and T. vulgare on C. elegans organism consists in hyperactivation of nicotinic cholinoreceptors. The possibility of quick adaptation of C. elegans nicotinic cholinoreceptors to active components of tansy flowers extract was revealed. Soil nematode C. elegans may be used for investigation of anthelmintic activity of A. sativum and T. vulgare for the purpose of identification of secondary metabolites responsible for the toxic action on helminths.

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Section: Resultsmentioning

confidence: 64%

Soil nematodeCaenorhabditis elegansMaupas as a convenient model organism to study anthelmintic activity of plant extracts

et al. 2020

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“…All experiments were carried out in M9 buffer (3 g/l KH2PO4, 6 g/l Na2HPO4, 5 g/l NaCl, 1 мМ MgSO4) [19]. After washing away from growth media, bacteria and exomethabolites [20] nematodes were transferred into glass centrifuge tubes 10 ml in volume (50 worms in each tube). After worms' settling on tubes' bottom supernatant was removed, extracts from D. filix-mas were added, and M9 buffer was added to final volume of 1 ml.…”

Section: In Vitro Assays Of Toxicity Of Dryopteris Filix-mas Roots An...mentioning

confidence: 99%

Toxic action of substances from male fern Dryopteris filix-mas (L.) Schott (1834) on free-living soil nematode Caenorhabditis elegans Maupas (1900)

et al. 2021

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The study of biological activity of extracts from roots and rhizomes of male fern Dryopteris filix-mas was carried out in experiments with soil nematode Caenorhabditis elegans. The toxicity of extracts of D. filix-mas roots and rhizomes obtained by different methods varied over a wide range. Crude extract of male fern roots and rhizomes in concentration range 62.5–250 µg/ml had a weak toxic action on C. elegans organism by inducing death of 26.0–43.3% nematodes. The toxicity of relatively high concentrations (500 and 250 µg/ml) of water-ethanolic extracts might be compared with such of crude extract in concentrations 62.5–250 µg/ml. Biological activity of filicinic acids ethers was found very high: concentrations of crude extract of D. filix-mas roots and rhizomes and concentrations of filicinic acids ethers with similar toxicity for C. elegans organism were 40-fold different. Substances isolated from D. filix-mas roots and rhizomes have nematicidal activity and may be used for development of drugs to treat helminthiasis of humans and animals.

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Heat Stress and Agonists of Muscarinic Cholinergic Receptors Modulate Sensitivity of Nicotinic Cholinergic Receptors in Soil Nematode Caenorhabditis elegans

et al. 2017

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We studied the effect of moderate heat stress (30oC) and muscarinic cholinergic receptor agonists arecoline and pilocarpine on sensitivity of the behavior of the nematode Caenorhabditis elegans of N2 line to the action of the agonist of nicotinic cholinergic receptor agonist levamisole. Heat stress and muscarinic cholinergic receptor agonists increased the sensitivity of swimming induced by mechanical stimulation to levamisole (32-64 μM), which manifested in dyscoordination of locomotor muscles during swimming and complete loss of ability to swim. Combined exposure to heat stress and muscarinic cholinergic receptor agonists revealed their synergism in the influence on sensitivity of swimming behavior to levamisole: heating to 30oC potentiated the effect of arecoline and arecoline potentiated the effect of heat stress. It is assumed, that the effect of heat stress on the sensitivity of nicotinic receptors is mediated by its effect on muscarinic receptors.

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Opposite effects of moderate heat stress and hyperthermia on cholinergic system of soil nematodes Caenorhabditis elegans and Caenorhabditis briggsae

Cited by 9 publications

References 69 publications

Soil nematodeCaenorhabditis elegansMaupas as a convenient model organism to study anthelmintic activity of plant extracts

Soil nematodeCaenorhabditis elegansMaupas as a convenient model organism to study anthelmintic activity of plant extracts

Toxic action of substances from male fern Dryopteris filix-mas (L.) Schott (1834) on free-living soil nematode Caenorhabditis elegans Maupas (1900)

Heat Stress and Agonists of Muscarinic Cholinergic Receptors Modulate Sensitivity of Nicotinic Cholinergic Receptors in Soil Nematode Caenorhabditis elegans

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