Opposite Effects of Flexible Single-Stranded DNA Regions and Rigid Loops in DNAzyme on Colloidal Nanoparticle Stability for “Turn-On” Plasmonic Detection of Lead Ions

Diao, Wenhui; Wang, Guoqing; Wang, Luyang; Zhang, Lan; Ding, Shansen; Takarada, Tohru; Maeda, Mizuo; Liang, Xingguo

doi:10.1021/acsabm.0c00873

Cited by 31 publications

(15 citation statements)

References 43 publications

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Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

“…In another approach, Diao et al developed a highly sensitive DNAzyme-based mechanism for Pb 2+ detection using a “turn-on” strategy, as depicted in Figure c . Briefly, the system consisted of a thiolated Pb 2+ -dependent DNAzyme bonded to gold nanoparticles, which were subjected to hybridization of the substrate strand.…”

Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

“…In another approach, Diao et al developed a highly sensitive DNAzyme-based mechanism for Pb 2+ detection using a "turnon" strategy, as depicted in Figure 5c. 108 Briefly, the system consisted of a thiolated Pb 2+ -dependent DNAzyme bonded to gold nanoparticles, which were subjected to hybridization of the substrate strand. Thus, when hybridization occurred, base stacking caused the nanoparticles to aggregate, which in turn decreased their localized surface plasmon resonance (LSPR).…”

Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

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DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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Since their discovery almost three decades ago, DNAzymes have been used extensively in biosensing. Depending on the type of DNAzyme being used, these functional oligonucleotides can act as molecular recognition elements within biosensors, offering high specificity to their target analyte, or as reporters capable of transducing a detectable signal. Several parameters need to be considered when designing a DNAzyme-based biosensor. In particular, given that many of these biosensors immobilize DNAzymes onto a sensing surface, selecting an appropriate immobilization strategy is vital. Suboptimal immobilization can result in both DNAzyme detachment and poor accessibility toward the target, leading to low sensing accuracy and sensitivity. Various approaches have been employed for DNAzyme immobilization within biosensors, ranging from amine and thiol-based covalent attachment to non-covalent strategies involving biotin–streptavidin interactions, DNA hybridization, electrostatic interactions, and physical entrapment. While the properties of each strategy inform its applicability within a proposed sensor, the selection of an appropriate strategy is largely dependent on the desired application. This is especially true given the diverse use of DNAzyme-based biosensors for the detection of pathogens, metal ions, and clinical biomarkers. In an effort to make the development of such sensors easier to navigate, this paper provides a comprehensive review of existing immobilization strategies, with a focus on their respective advantages, drawbacks, and optimal conditions for use. Next, common applications of existing DNAzyme-based biosensors are discussed. Last, emerging and future trends in the development of DNAzyme-based biosensors are discussed, and gaps in existing research worthy of exploration are identified.

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Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

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DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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“…Starting with analytes possessing simple chemical structure such as heavy metals ( e.g. , Hg 2+ , Pb 2+ ) and melamine, researchers have design various sensing systems based on their well-understood interactions with DNA, 201 DNAzyme 205 and peptides 207 (for heavy metals) as well as citrate, 219 methanobactin 217 and DTT, 173 etc. (for melamine).…”

Section: Trends and Challenges Of Gold Nanoparticles-based Optical Se...mentioning

confidence: 99%

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

et al. 2022

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“…Target DNA-induced base pair stacking assembly of DNA-functionalized gold nanoparticles (DNA-AuNPs) accompanied by the red-to-purple color change induced by the interparticle plasmon coupling has suggested its applicability in facile identification of short DNA oligonucleotides. − In addition, we have quite recently found that this assembly takes place not only in aqueous solutions, but also in complicated matrices . The aim of the present work is to examine whether exogeneous short DNA oligonucleotides (DNA markers) in liquid foods can be detected using DNA-AuNPs for traceability.…”

Section: Introductionmentioning

confidence: 99%

Identifying Exogenous DNA in Liquid Foods by Gold Nanoparticles: Potential Applications in Traceability

Ding

Wang

et al. 2021

ACS Food Sci. Technol.

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Quality control of food products requires the development of powerful analytical tools and regulations to avoid fraud to consumers and ineffective product recalls. DNA barcoding represents a potential strategy for application in food traceability, which involves the use of exogeneous short DNA oligonucleotides (DNA markers) for tracing the origin of food, such as suppliers, producers, attributes, and points of distribution. However, it is generally considered that exogeneous DNA molecules are strongly shielded by complicated interactions with intrinsic ingredients and easily degraded in complex food systems. This consideration leads to the lack of analytical methods to readily identify DNA markers in food, thereby preventing one from exploiting the barcoding capability of DNA markers in food. In this work, we explored the applicability of DNA markers coupled with rapid DNA detection by gold nanoparticles to traceability of various liquid foods, including wines, condiments, and soft drinks. Our results revealed that the gold nanoparticles afford colorimetric identification of DNA markers in liquors, condiments, and milk with a quick (≤5 min) “yes” or “no” readout depending on the solution color. Importantly, the DNA markers stored in these liquid foods remain chemically stable and bioactive in hybridization within months, thereby allowing for barcoding liquid foods with considerably long shelf lives. Given the nutritional value of nucleic acids and the direct DNA identification avoiding complicated isolation and analysis, the present work could provide a practical approach to food traceability.

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Opposite Effects of Flexible Single-Stranded DNA Regions and Rigid Loops in DNAzyme on Colloidal Nanoparticle Stability for “Turn-On” Plasmonic Detection of Lead Ions

Cited by 31 publications

References 43 publications

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

Identifying Exogenous DNA in Liquid Foods by Gold Nanoparticles: Potential Applications in Traceability

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