2023
DOI: 10.1007/s00018-022-04672-w
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Opposing USP19 splice variants in TGF-β signaling and TGF-β-induced epithelial–mesenchymal transition of breast cancer cells

Abstract: Ubiquitin-specific protease (USP)19 is a deubiquitinating enzyme that regulates the stability and function of multiple proteins, thereby controlling various biological responses. The alternative splicing of USP19 results in the expression of two major encoded variants that are localized to the endoplasmic reticulum (ER) (USP19-ER) and cytoplasm (USP19-CY). The importance of alternative splicing for the function of USP19 remains unclear. Here, we demonstrated that USP19-CY promotes TGF-β signaling by directly i… Show more

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Cited by 7 publications
(5 citation statements)
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“…Of note, TGFβ ligand is an essential factor of EndoMT and induces SERPINE1 expression, which is a molecular switch controlling cardiac TGFβ signaling. 29,30…”
Section: Resultsmentioning
confidence: 99%
“…Of note, TGFβ ligand is an essential factor of EndoMT and induces SERPINE1 expression, which is a molecular switch controlling cardiac TGFβ signaling. 29,30…”
Section: Resultsmentioning
confidence: 99%
“…To investigate the TGF‐β‐induced changes in GSL‐glycans in cells undergoing EMT, we chose two established cell models with prominent TGF‐β‐induced EMT responses, i.e., NMuMG (Zhang et al , 2020a) and A549‐vimentin (VIM)‐red fluorescent protein (RFP) cell lines (Wang et al , 2020). We performed quantitative glycomic analysis of these cell lines using porous graphitized carbon (PGC) nano‐liquid chromatography‐electrospray ionization tandem mass spectrometry (nano‐LC‐ESI‐MS/MS) in negative ESI mode, allowing in‐depth determination of GSL profiles (Appendix Fig S1; Anugraham et al , 2015).…”
Section: Resultsmentioning
confidence: 99%
“…Activation of EMT can endow cells with an enhanced ability to migrate (Zhang et al , 2020a). We thus further investigated the basal migration rate upon Ugcg KO in NMuMG cells.…”
Section: Resultsmentioning
confidence: 99%
“…Even though there was evidence connecting USP19 to the progression of BC, only a handful of the underlying molecular pathways have been discussed. [11][12][13] ER is an important organelle responsible for protein processing, modification and folding, and plays a crucial role in cell Apoptosis. 14 Different oncogenic factors, as well as metabolic and transcriptional abnormalities in the tumor microenvironment cooperate to disrupt ER homeostasis and immune cell infiltration, which trigger a persistent state of ER stress.…”
Section: Introductionmentioning
confidence: 99%
“…found that C607S, an enzyme‐inactive mutant site of USP19 , failed to inhibit TNFA and IL‐1β‐triggered NF‐κB activation after mutation. Even though there was evidence connecting USP19 to the progression of BC, only a handful of the underlying molecular pathways have been discussed 11–13 …”
Section: Introductionmentioning
confidence: 99%