Opposing Functions of Calcineurin and CaMKII Regulate G-protein Signaling in Egg-laying Behavior of C.elegans

Lee, Jungsoo; Jee, Chang Hoon; Song, Hyun Ok; Bandyopadhyay, Jaya; Lee, Jin I.; Yu, Jeong Jin; Park, Byung Jae; Ahnn, Joohong

doi:10.1016/j.jmb.2004.09.050

Cited by 31 publications

(27 citation statements)

References 32 publications

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“…Since only the shn-1(tm488) mutant allele displayed defecation defect, we constructed a shn-1(tm488);itr-1(sa73)(lf) double mutant and observed its defecation behavior. As described previously (Dal Santo et al, 1999;Lee et al, 2004), itr-1(sa73)(lf) mutant showed much prolonged defecation intervals as compared to wild-type animal (Table 1). Interestingly, we found that shn-1(tm488);itr-1(sa73) (lf) double mutant showed a similar defecation interval to that of itr-1(sa73)(lf) single mutant (Table 1).…”

Section: Resultssupporting

confidence: 77%

“…Hermaphrodite fertility was analyzed by measuring brood size. The brood size was measured by counting progenies (F 1 ) after placing and daily transferring individual worms (P 0 ) on seeded plates at 16°C, 20°C and 25°C as described (Kim et al, 2008;Lee et al, 2004). Male fertility was analyzed by mating efficiency test, sperm competition and male mating behavior.…”

Section: Phenotype Analysismentioning

confidence: 99%

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ANK Repeat-Domain of SHN-1 Is Indispensable for In Vivo SHN-1 Function in C. elegans

Song

Cho

et al. 2011

Molecules and Cells

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Shank protein is one of the postsynaptic density (PSD) proteins which play a major role in proper localization of proteins at membranes. The shn-1, a homolog of Shank in Caenorhabditis elegans, is expressed in neurons, pharynx, intestine, vulva and sperm. We have previously reported a possible genetic interaction between Shank and IP 3 receptor by examining shn-1 RNAi in IP 3 receptor (itr-1) mutant background. In order to show the direct interaction of Shank and IP 3 receptor as well as to show the direct in vivo function of Shank, we have characterized two different mutant alleles of shn-1, which have different deletions in the different domains. shn-1 mutants were observed for Ca 2+-related behavioral defects with itr-1 mutants. We found that only shn-1 mutant defective in ANK repeatdomain showed significant defects in defecation, pharyngeal pumping and fertility. In addition, we found that shn-1 regulates defecation, pharyngeal pumping and probably male fertility with itr-1. Thus, we suggest that Shank ANK repeat-domain along with PDZ may play a crucial role in regulating Ca 2+-signaling with IP 3 receptor.

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Section: Resultssupporting

confidence: 77%

Section: Phenotype Analysismentioning

confidence: 99%

ANK Repeat-Domain of SHN-1 Is Indispensable for In Vivo SHN-1 Function in C. elegans

Song

Cho

et al. 2011

Molecules and Cells

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“…A role for CaMKII in meiosis resumption has not been explored in this organism; however, CaN has been implicated in the completion of meiosis through its regulator, Calcipressin (Horner et al, 2006;Takeo et al, 2006). Mutations in the Drosophila Calcipressin, sarah (sra), result in an anaphase I arrest with high levels of cyclin B (Horner et al, 2006;Takeo et al, 2006 (Fuentes et al, 2000;Kingsbury and Cunningham, 2000;Lee et al, 2004;Chan et al, 2005), but at endogenous levels, Calcipressin has a stimulatory effect on CaN activity (Hilioti and Cunningham, 2003;Vega et al, 2003). During Drosophila egg activation, it is likely that Sra acts to stimulate CaN activity, because CaN mutants also arrest at anaphase I (S. Takeo and T. Aigaki, personal communication).…”

Section: Meiosis Arrest and Release In Drosophilamentioning

confidence: 99%

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

Horner

Wolfner

2008

Developmental Dynamics

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The transition from mature oocyte to developing embryo requires a coordinated series of events, collectively known as egg activation. Egg activation includes changes to egg coverings to prevent polyspermy, release of oocyte meiotic arrest, generation of haploid female and male pronuclei, changes in maternal mRNAs and protein populations, and cytoskeletal rearrangements. In many animals, egg activation is triggered by fertilization, which increases intracellular calcium within the oocyte and thereby regulates molecular events of egg activation. In other animals, fertilization-independent external signals, including mechanical stimulation of eggs and/or changes in ionic milieu, trigger activation. Recent studies have clarified the upstream portion of pathways leading to eggshell changes and cell cycle resumption and have identified activation-induced changes in maternal mRNA and protein profiles that can identify molecular players in the downstream events of egg activation. We review signals that trigger activation and how they link to subsequent molecular events of egg activation. Developmental Dynamics 237:527-544, 2008.

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“…It is extensively expressed in neurons as well as other tissues, including hypodermal seam cells, body-wall muscle, vulva muscle, sperm, and the spermatheca (11)(12)(13). Studies have revealed that calcineurin functions in regulating various Ca 2+ -dependent signaling pathways, including thermotaxis, chemotaxis, locomotion, egg-laying behavior, and defecation in C. elegans (11)(12)(13). In addition, we have previously shown that calcineurin regulates endocytosis in coelomocytes, which are scavenger cells located in the body cavity of C. elegans (14).…”

Section: Introductionmentioning

confidence: 99%

Calcineurin may regulate multiple endocytic processes in C. elegans

Song

Ahnn²

2011

BMB Reports

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Calcineurin is a serine/threonine protein phosphatase controlled by Ca 2+ and calmodulin that has been implicated in various signaling pathways. Previously, we reported that calcineurin regulates coelomocyte endocytosis in Caenorhabditis elegans. So far, simple and powerful in vivo approaches have been developed to study various endocytic processes in C. elegans. Using these in vivo assays, we further analyzed the endocytic phenotypes of calcineurin mutants. We observed that the calcineurin mutants were defective in apical endocytosis in the intestine as well as synaptic vesicle recycling in the nerve cord. However, we found that calcineurin mutants displayed normal receptor-mediated endocytosis in oocytes. Therefore, our results suggest that calcineurin may regulate specific sets of endocytic processes in nematode. [BMB reports 2011; 44(2): 96-101]

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Opposing Functions of Calcineurin and CaMKII Regulate G-protein Signaling in Egg-laying Behavior of C.elegans

Cited by 31 publications

References 32 publications

ANK Repeat-Domain of SHN-1 Is Indispensable for In Vivo SHN-1 Function in C. elegans

ANK Repeat-Domain of SHN-1 Is Indispensable for In Vivo SHN-1 Function in C. elegans

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

Calcineurin may regulate multiple endocytic processes in C. elegans

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