Opposing effects of endothelin-1 on C-type natriuretic peptide actions in rat cardiomyocytes

Fixler, Ruhama; Hasin, Yonathan; Eilam, Y.; Zhang, Dong Fang; Nir, Amiram

doi:10.1016/s0014-2999(01)01094-9

Cited by 13 publications

(4 citation statements)

References 11 publications

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“…n=number of incubated aliquots, number of experiments. Miura et al 1995, Barker & Corder 1997, Hillier et al 2001, and can modify the response of vasoactive peptide at the target cell (Eguchi et al 1994, Vigne et al 1994, Fixler et al 2001, suggest the existence of a finely tuned, self-responsive vasoregulatory system with an important component residing within endothelial cells. The media were not changed after 1 h pretreatment incubation, and normal medium or medium containing bosentan was pipetted into the incubation.…”

Section: Discussionmentioning

confidence: 99%

Effects of endothelin-1 on release of adrenomedullin and C-type natriuretic peptide from individual human vascular endothelial cells

Ah²,

Yandle³

et al. 2002

Journal of Endocrinology

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Regulation of cardiovascular system activity involves complex interactions amongst numerous factors. Three of these vasoactive factors are adrenomedullin, C-type natriuretic peptide (CNP) and endothelin-1 (ET-1), each of which is claimed to have important local effects. To investigate paracrine/autocrine regulation of the secretion of these peptides we used a cell immunoblot method. We postulated that basal release of adrenomedullin and CNP by endothelial cells is modulated by ET-1. Dispersed human aortic endothelial cells were attached to a protein binding membrane and incubated for 1 or 4 h with control medium or with ET-1, endothelin receptor antagonists or antibody to ET-1, and then submitted to immunohistochemical staining. Peptides (adrenomedullin, CNP and ET-1) within individual cells were stained, as was peptide secreted and adjacent to the cell. It was demonstrated that adrenomedullin, CNP and ET-1 can be contained within the same cell. In addition, we observed that individual endothelial cells can secrete all three peptides.The endothelin ET-A/ET-B receptor antagonist, bosentan, the ET-B receptor antagonist, BQ-788, and anti-ET-1 serum decreased the percentage of endothelial cells that secreted adrenomedullin and CNP relative to control. Conversely, the addition of ET-1 induced an increase in the number of endothelial cells that secreted adrenomedullin and CNP. These results provide strong evidence that endogenous ET-1, from human vascular endothelial cells, acts in a paracrine/autocrine manner to modulate the basal release of adrenomedullin and CNP. Our observations of this modulation suggest that vascular endothelial cells of humans constitute an important component of a self-responsive vasoregulatory system.

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Section: Discussionmentioning

confidence: 99%

Effects of endothelin-1 on release of adrenomedullin and C-type natriuretic peptide from individual human vascular endothelial cells

Ah²,

Yandle³

et al. 2002

Journal of Endocrinology

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“…ET-1 inhibits ANP-dependent cGMP accumulation in pulmonary artery endothelial cells (459). In cardiac myocytes, CNP decreased contractility and increased cGMP, while ET-1 inhibited these effects (205, 778). ET-1 can downregulate ANP-C receptors in A-10 smooth muscle cells (65).…”

Section: Endothelin and Humoral Systemsmentioning

confidence: 99%

Regulation of Blood Pressure and Salt Homeostasis by Endothelin

Kohan

Rossi

Inscho

et al. 2011

Physiological Reviews

367

459

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Endothelin (ET) peptides and their receptors are intimately involved in the physiological control of systemic blood pressure and body Na homeostasis, exerting these effects through alterations in a host of circulating and local factors. Hormonal systems affected by ET include natriuretic peptides, aldosterone, catecholamines, and angiotensin. ET also directly regulates cardiac output, central and peripheral nervous system activity, renal Na and water excretion, systemic vascular resistance, and venous capacitance. ET regulation of these systems is often complex, sometimes involving opposing actions depending on which receptor isoform is activated, which cells are affected, and what other prevailing factors exist. A detailed understanding of this system is important; disordered regulation of the ET system is strongly associated with hypertension and dysregulated extracellular fluid volume homeostasis. In addition, ET receptor antagonists are being increasingly used for the treatment of a variety of diseases; while demonstrating benefit, these agents also have adverse effects on fluid retention that may substantially limit their clinical utility. This review provides a detailed analysis of how the ET system is involved in the control of blood pressure and Na homeostasis, focusing primarily on physiological regulation with some discussion of the role of the ET system in hypertension.

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“…CNP was shown to have a direct positive chronotropic [ 88 ] and dromotropic [ 89 ] effect in anaesthetized dogs. Studies in cultured neonatal rat CMs showed negative inotropic effects caused by CNP stimulation [ 90 , 91 ]. In rat papillary muscles, CNP exerted a positive lusitropic and a negative inotropic effect [ 92 ].…”

Section: Compartmentation Of Cgmp Signaling In the Cardiovascular mentioning

confidence: 99%

cGMP Signaling in the Cardiovascular System—The Role of Compartmentation and Its Live Cell Imaging

Bork

Nikolaev

2018

IJMS

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The ubiquitous second messenger 3′,5′-cyclic guanosine monophosphate (cGMP) regulates multiple physiologic processes in the cardiovascular system. Its intracellular effects are mediated by stringently controlled subcellular microdomains. In this review, we will illustrate the current techniques available for real-time cGMP measurements with a specific focus on live cell imaging methods. We will also discuss currently accepted and emerging mechanisms of cGMP compartmentation in the cardiovascular system.

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Opposing effects of endothelin-1 on C-type natriuretic peptide actions in rat cardiomyocytes

Cited by 13 publications

References 11 publications

Effects of endothelin-1 on release of adrenomedullin and C-type natriuretic peptide from individual human vascular endothelial cells

Effects of endothelin-1 on release of adrenomedullin and C-type natriuretic peptide from individual human vascular endothelial cells

Regulation of Blood Pressure and Salt Homeostasis by Endothelin

cGMP Signaling in the Cardiovascular System—The Role of Compartmentation and Its Live Cell Imaging

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