Opposed cannabinoid 1 receptor (CB1R) expression in the prefrontal cortex vs. nucleus accumbens is associated with alcohol consumption in male rats

Amancio-Belmont, Octavio; Meléndez, Alline L. Becerril; Ruiz-Contreras, Alejandra E.; Méndez-Dı́az, Mónica; Prospéro-Garcı́a, Oscar

doi:10.1016/j.brainres.2019.146485

Cited by 10 publications

(5 citation statements)

References 54 publications

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“…Some of these data differ from anterior reports concerning expression of CBR, nonetheless most of those reports used different techniques and did not measure the protein conforming CBR’s but mostly mRNA [21,46,47], which highlights the novelty of the data presented in this work, by showing a direct measure of the expressed protein in question under this particularly conditions of exercise. On the same line, the increased expression of CBR in splenocytes, particularly in lymphocytes from the EXE group, concurs with previous data reporting changes in the expression of the protein through the assessment by different techniques, like western blot and immunofluorescense [21,48,49], adding validity to our results.…”

Section: Discussionsupporting

confidence: 93%

Chronic exercise modulates the cellular immunity and its cannabinoid receptors expression

Valencia-Sánchez

Nava-Castro²,

Palacios-Arreola³

et al. 2019

PLoS ONE

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The impact of performing exercise on the immune system presents contrasting effects on health when performed at different intensities. In addition, the consequences of performing chronic exercise have not been sufficiently studied in contrast to the effects of acute bouts of exercise. The porpoise of this work was to determine the effect that a popular exercise regimen (chronic/moderate/aerobic exercise) has on the proportion of different immune cell subsets, their function and if it affects the cannabinoid system with potentially functional implications on the immune system. A marked increase in several immune cell subsets and their expression of cannabinoid receptors was expected, as well as an enhanced proliferative and cytotoxic activity by total splenocytes in exercised animals. For this study male Wistar rats performed treadmill running 5 times a week for a period of 10 weeks, at moderate intensity. Our results showed a significant decrease in lymphocyte subpopulations (CD4+, Tγδ, and CD45 RA+ cells) and an increase in the cannabinoid receptors expression in those same cell. Although functional assays did not reveal any variation in total immunoglobulin production or NK cells cytotoxic activity, proliferative capability of total splenocytes increased in trained rats. Our results further support the notion that exercise affects the immunological system and extends the description of underlying mechanisms mediating such effects. Altogether, our results contribute to the understanding of the benefits of exercise on the practitioner´s general health.

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Section: Discussionsupporting

confidence: 93%

Chronic exercise modulates the cellular immunity and its cannabinoid receptors expression

Valencia-Sánchez

Nava-Castro²,

Palacios-Arreola³

et al. 2019

PLoS ONE

Self Cite

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“…Hence, MS may be a valid paradigm for the evaluation of drug addiction therapies in traumatized children. 4 MD in rodents is a viable model of ELS that causes significant anxiety, motivation, 38 and cognitive 50 changes, which may enhance sensitivity to alcohol 38 and drug dependence.…”

Section: Resultsmentioning

confidence: 99%

“…Early life trauma rewires the brain’s dopamine and endocannabinoid circuits, making people more susceptible to anxiety, alcohol, and drug dependence. 38 , 39 …”

Section: Resultsmentioning

confidence: 99%

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Maternal Substance Use and Early-Life Adversity: Inducing Drug Dependence in Offspring, Interactions, Mechanisms, and Treatments

Fadaei-Kenarsary,

Esmaeilpour,

Shabani

et al. 2024

Addict Health

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The likelihood of substance dependency in offspring is increased in cases when there is a family history of drug or alcohol use. Mothering is limited by maternal addiction because of the separation. Maternal separation (MS) leads to the development of behavioural and neuropsychiatric issues in the future. Despite the importance of this issue, empirical investigations of the influences of maternal substance use and separation on substance use problems in offspring are limited, and studies that consider both effects are rare. This study aims to review a few studies on the mechanisms, treatments, genetics, epigenetics, molecular and psychological alterations, and neuroanatomical regions involved in the dependence of offspring who underwent maternal addiction and separation. The PubMed database was used. A total of 95 articles were found, including the most related ones in the review. The brain’s lateral paragigantocellularis (LPGi), nucleus accumbens (NAc), caudate-putamen (CPu), prefrontal cortex (PFC), and hippocampus, can be affected by MS. Dopamine receptor subtype genes, alcohol biomarker minor allele, and preproenkephalin mRNA may be affected by alcohol or substance use disorders. After early-life adversity, histone acetylation in the hippocampus may be linked to brain-derived neurotrophic factor (BDNF) gene epigenetics and glucocorticoid receptors (GRs). The adverse early-life experiences differ in offspring›s genders and rewire the brain›s dopamine and endocannabinoid circuits, making offspring more susceptible to dependence. Related psychological factors rooted in earlylife stress (ELS) and parental substance use disorder (SUD). Treatments include antidepressants, histone deacetylase inhibitors, lamotrigine, ketamine, choline, modafinil, methadone, dopamine, cannabinoid 1 receptor agonists/antagonists, vitamins, oxytocin, tetrahydrocannabinol, SR141716A, and dronabinol. Finally, the study emphasizes the need for multifaceted strategies to prevent these outcomes.

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“…The results demonstrated that the expression of CB1R in neuron cells was signi cantly higher than that in BV2 cells (P = 0.0039, N = 3), con rming the antibody's speci city. It is also observed that CB1R is expressed in both PFC and hippocampus of mice [26,37]. To further con rm the speci city of CB1R antibody, immuno uorescence staining for CB1R was conducted in PFC of mice and monkey, revealing positive signals for CB1R in both species (Fig.…”

Section: Cb1r Is Expressed In the Pfc Of Monkeysmentioning

confidence: 92%

Laminar Distribution of Cannabinoid Receptor 1 in the Prefrontal Cortex of Nonhuman Primates

Han,

Dong,

Peng

et al. 2023

Mol Neurobiol

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Cannabis is an annual herb of the genus Cannabis, with a history of medical use going back thousands of years. However, its abuse causes many side-effects, including confusion of consciousness, alienation, and mental disorders such as schizophrenia and depression. Research conducted on rodents suggests that there are two types of cannabinoid receptors -cannabinoid receptor 1 (CB1R) and cannabinoid receptor 2 (CB2R). CB1R is found mostly in the central nervous system, particularly in the prefrontal cortex (PFC), and alterations in its expression in the PFC have been strongly linked to mental disorders.Within the layers of the PFC, Brodmann area 46 is associated with the processing of complex cognitive information. However, it remains unclear whether CB1R is expressed in the PFC 46 area of non-human primate. In this work, we applied western blotting along with immuno uorescent histochemical staining to investigate the distribution pattern of CB1R in the PFC of nonhuman primate, Our ndings reveal that CB1R is highly expressed in the monkey PFC, especially in area 46. Furthermore, CB1R exhibits a layered distribution pattern within area 46 of the PFC, with the inner granular layer displaying the highest expression levels. Additionally, CB1R + PV + cells are widely distributed in lay -of area 46, with layer IV showing notable prevalence. In conclusion, CB1R is distributed in the PV interneurons in area 46 of the prefrontal cortex, particularly in layer , suggesting that cannabis may modulate PFC activities via regulating interneuron in the PFC. And cannabis-induced side effects may be caused by abnormal expression of CB1R.

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Opposed cannabinoid 1 receptor (CB1R) expression in the prefrontal cortex vs. nucleus accumbens is associated with alcohol consumption in male rats

Cited by 10 publications

References 54 publications

Chronic exercise modulates the cellular immunity and its cannabinoid receptors expression

Chronic exercise modulates the cellular immunity and its cannabinoid receptors expression

Maternal Substance Use and Early-Life Adversity: Inducing Drug Dependence in Offspring, Interactions, Mechanisms, and Treatments

Laminar Distribution of Cannabinoid Receptor 1 in the Prefrontal Cortex of Nonhuman Primates

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