Opportunities for marine bioprocess intensification using novel bioreactor design: frequency of barotolerance in microorganisms obtained from surface waters

Wright, Phillip C.; Stevenson, Colin; McEvoy, Eileen; Burgess, J. Grant

doi:10.1016/s0168-1656(99)00086-3

Cited by 14 publications

(7 citation statements)

References 14 publications

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“…Indeed, bacteria associated with surface film seem to have a higher barotolerance than those not associated with surface film, so they may be able to withstand greater pressures (Wright et al, 1999). The pressure tolerance could be harnessed in a hydrostatic pressure bioreactor to improve O 2 transfer (Zobell and Hittle, 1967) via higher oxygen partial pressure (Enns et al, 1965) without increasing shear stresses.…”

Section: Pressurementioning

confidence: 99%

“…Hydrostatic pressurization to relatively low pressures can be achieved with only minor alterations to a "standard" bioreactor, but can markedly improve parameters such as maximum cell dry weight (Yang and Wang, 1992). Furthermore, the effect of pressure may act as a stressor to increase metabolite production via Le Châtelier's principle, if a negative volume change in reactants to products is favored (Wright et al, 1999). The effect of small pressure increases on secondary metabolism of nonbarophillic bacteria has not been investigated, with most biotechnology work concentrating on barophiles (e.g., Nelson et al, 1992).…”

Section: Pressurementioning

confidence: 99%

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Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

1999

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: There is a lack of research into bioreactor engineering and fermentation protocol design in the field of marine bacterial antibiotic production. Most production strategies are carried out at the shake-flask level and lack a mechanistic understanding of the antibiotic production process, offering poor prospects for successful scale-up. This review shows that data need to be collated on media and physical optima differences between the trophophase and idiophase, along with investigations into the control mechanisms for biosynthesis, to allow implementation of novel fermentation protocols. Immobilization may play a part in bioprocess intensification of marine bacterial antibiotic production, through again this area is understudied. Similarly, mass transfer and shear stress data of fermentations are needed to provide the bioreactor design requirements to intensify antibiotic biosynthesis, with process scale-up in mind. The application of bioprocess intensification methods to the production of antibiotics (and other metabolites) from marine microbes will become an important strategy for improving supply of natural products, in order to assess their suitability as chemotherapeutic drugs.

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Section: Pressurementioning

confidence: 99%

Section: Pressurementioning

confidence: 99%

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

1999

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“…The isolated strains were further exposed to high pressure of 3.1 MPa under absolute O 2 atmosphere in a high pressure reactor (Autoclave Engineers) for a 1-h period and their survival percentage were determined (Wright et al 1999).…”

Section: Methodsmentioning

confidence: 99%

Poly-extremotolerant bacterium isolated from reverse osmosis reject: an implication toward waste water management

Jain¹,

Mishra²,

Shrivastav³

et al. 2010

Folia Microbiol

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We demonstrate the tolerance of bacterial strain SM2014 to various unsustainable conditions and suggest its implication in waste water management. Its sustainability to reverse osmosis pressure (2.1 MPa) during desalination, and survival percentage of 73 % under hyperbaric conditions (pressure tension of 3.1 MPa under absolute oxygen atmosphere) confirmed its pressure tolerance. The growth of this strain at pH 9 or 10 and at 60 °C alone or in combination revealed its unique physiology as poly-extremotolerant strain. As an adaptive mechanism, the ratio of saturated to unsaturated fatty acids changed with growth conditions. Under poly-extreme condition long chain saturated fatty acid (C₁₈:₀, C₁₆:₀, C₁₄:₀, C₁₂:₀) predominated at the expense of unsaturated fatty acids. The nucleotide BLAST of 16S rRNA gene sequence of strain SM2014 with the NCBI gene bank sequences showed its close identity to Bacillus licheniformis with a similarity match of 94 %. The secretion of industrially valuable enzymes proteinase, lipase and amylase under such harsh conditions further signified potential of this strain as a source of extremozymes. Its unique characteristics underscore its relevance in waste water management.

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“…Additionally, Miller et al 78 observed that high pressures associated with natural growth conditions of marine hyperthermophiles, isolated from deep‐sea vents, were influential, although most could be cultured under ambient pressures. Furthermore, this has led to the development of high‐pressure bioreactors and investigation into the survival, yield and selectivity effects of antibiotic production under high hydrostatic pressure 79.…”

Section: Technology and Industrial Development Of Marine Hyperthermmentioning

confidence: 99%

Novel opportunities for marine hyperthermophiles in emerging biotechnology and engineering industries

Bustard

Burgess

Meeyoo

et al. 2000

J. Chem. Technol. Biotechnol.

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Despite the increased interest in hyperthermophiles and the rate of discovery of new species, their potential within the biotechnology industry has not been fully realized to date. The physiological characterization and biochemical survival mechanisms of marine hyperthermophilic Bacteria and Archaea are currently under investigation. However, very little information on their application in bioprocess systems is available. Since only a fraction of the world's oceans has been explored, the potential for isolating novel strains of marine hyperthermophiles is signi®cant and hence they represent an, as yet, untapped biotechnological resource. Although much research has focused on the extraction of thermoactive enzymes, whole cell applications have been relatively overlooked. Running bioprocess systems using marine hyperthermophiles poses an interesting set of objectives, such as high temperature bioreactor operation and corrosion reduction of materials, for bioreactor design and manipulation of their products. Here, we discuss the biotechnological potential of marine hyperthermophiles from a biochemical engineering perspective and their use in`green chemistry' applications. Both the bioprocess intensi®cation implications and problems associated with cultivating these microbes in industrially relevant bioreactor systems are discussed from both a microbiological and chemical engineering perspective. NOTATION aParameter of the Hougen±Watson rate expression (Pa À3/2 mol kg À1 s À1 ) bParameter of the Hougen±Watson rate expression (Pa À1/2 ) cParameter of the Hougen±Watson rate expression (Pa À1/2 ) [C glu ] initial Initial glucose concentration (mol dm À3 ) DG Gibb's free energy of reaction (kJ mol À1 ) DG stab Gibb's free energy of stabilization (kJ mol À1 ) DG 298Gibb's free energy at 298 K (kJ mol À1 ) DG 573Gibb's free energy at 573 K (kJ mol À1 ) DH Enthalpy of reaction (kJ mol À1 ) Kp CO2 Equilibrium constant for partial pressure of CO 2 (kPa 2 ) Kp H2O Equilibrium constant for partial pressure of H 2 O (kPa 2 )Kp H2 Equilibrium constant for partial pressure of H 2 (kPa 2 ) r CH4 Rate of methane production (mol kg À1 s À1 ) T max Maximum temperature for cell growth (°C)

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Opportunities for marine bioprocess intensification using novel bioreactor design: frequency of barotolerance in microorganisms obtained from surface waters

Cited by 14 publications

References 14 publications

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

Poly-extremotolerant bacterium isolated from reverse osmosis reject: an implication toward waste water management

Novel opportunities for marine hyperthermophiles in emerging biotechnology and engineering industries

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