Opportunistic microorganisms in individuals with lesions of denture stomatitis

Pereira, Cristiane Aparecida; Toledo, Bruna Costa; Santos, Camila Teles; Costa, Anna Carolina Borges Pereira; Back-Brito, Graziella Nuernberg; Kaminagakura, Estela; Jorge, Antônio Olavo Cardoso

doi:10.1016/j.diagmicrobio.2013.05.001

Cited by 81 publications

(94 citation statements)

References 43 publications

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“…The yeast Candida albicans is found as a commensal microorganism in the digestive tract of mammals [1], as well as in the oral cavity of humans [2]. It is also the most common fungal pathogen in humans, causing both mucosal and systemic infections, particularly in immunocompromised individuals [3].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Thiazolidinedione-8 as an Antibiofilm Agent against Candida albicans

et al. 2014

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Candida albicans is known as a commensal microorganism but it is also the most common fungal pathogen in humans, causing both mucosal and systemic infections. Biofilm-associated C. albicans infections present clinically important features due to their high levels of resistance to traditional antifungal agents. Quorum sensing is closely associated with biofilm formation and increasing fungal pathogenicity. We investigated the ability of the novel bacterial quorum sensing quencher thiazolidinedione-8 (S-8) to inhibit the formation of, and eradication of mature C. albicans biofilms. In addition, the capability of S-8 to alter fungal adhesion to mammalian cells was checked. S-8 exhibited specific antibiofilm and antiadhesion activities against C. albicans, at four- to eightfold lower concentrations than the minimum inhibitory concentration (MIC). Using fluorescence microscopy, we observed that S-8 dose-dependently reduces C. albicans–GFP binding to RAW macrophages. S-8 at sub-MICs also interfered with fungal morphogenesis by inhibiting the yeast-to-hyphal form transition. In addition, the tested agent strongly affected fungal cell wall characteristics by modulating its hydrophobicity. We evaluated the molecular mode of S-8 antibiofilm and antiadhesion activities using real-time RT-PCR. The expression levels of genes associated with biofilm formation, adhesion and filamentation, HWP1, ALS3 and EAP1, respectively, were dose-dependently downregulated by S-8. Transcript levels of UME6, responsible for long-term hyphal maintenance, were also significantly decreased by the tested agent. Both signaling pathways of hyphal formation-cAMP-PKA and MAPK-were interrupted by S-8. Their upstream general regulator RAS1 was markedly suppressed by S-8. In addition, the expression levels of MAPK cascade components CST20, HST7 and CPH1 were downregulated by S-8. Finally, transcriptional repressors of filament formation, TUP1 and NRG1, were dramatically upregulated by our compound. Our results indicate that S-8 holds a novel antibiofilm therapeutic mean in the treatment and prevention of biofilm-associated C. albicans infections.

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Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Thiazolidinedione-8 as an Antibiofilm Agent against Candida albicans

et al. 2014

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“…For instance, this microbe has been implicated as the primary pathogen in oral candidosis in HIV‐infected patients. In addition, when Candida ‐induced denture stomatitis and Candida species colonization were assessed in denture wearers, C. dubliniensis was recovered in several studies . Hence, it has been proposed that C. dubliniensis plays a crucial role in the establishment and persistence of C. dubliniensis induced‐denture stomatitis .…”

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confidence: 99%

Caspofungin‐induced in‐vitro post‐antifungal effect and its impact on adhesion related traits of oral Candida dubliniensis and Candida albicans isolates

Ellepola

Chandy

Khan

et al. 2016

Microbiology and Immunology

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Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation and cell surface hydrophobicity (CSH) are all virulence traits involved in the pathogenicity of Candida. Post-antifungal effect (PAFE) also have a bearing on pathogenicity and virulence of Candida. Candida dubliniensis is associated with oral and systemic candidosis, which can be managed with caspofungin. There is no published information on caspofungin-induced PAFE and its impact on adhesion traits of C. dubliniensis isolates. Thus, the purpose of this investigation was to determine the in vitro duration of PAFE on 20 C. dubliniensis isolates following transient exposure to caspofungin. Furthermore the impacts of caspofungin-induced PAFE on adhesion to BEC and DAS, GT formation and CSH of these isolates were also determined. After establishing the minimum inhibitory concentration (MIC) of caspofungin, C. dubliniensis isolates were exposed to sub-lethal concentrations (Â3 MIC) of caspofungin for 1 hr. Thereafter the duration of PAFE, adhesion to BEC and DAS, GT formation and CSH were determined by previously described in-vitro assays. MIC (mg/mL) of C. dubliniensis isolates to caspofungin ranged from 0.004 to 0.19. Caspofungin-induced mean PAFE on C. dubliniensis isolates was 2.17 hr. Exposure to caspofungin suppressed the ability of C. dubliniensis isolates to adhere to BEC and DAS, form GT and CSH by 69.97%, 71.95%, 90.06% and 32.29% (P < 0.001 for all), respectively. Thus, transient exposure of C. dubliniensis isolates to caspofungin produces an antifungal effect not only by suppressing its growth but also by altering its adhesion traits.

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“…he yeast Candida albicans is found as a commensal microorganism in the digestive tract of mammals (1), as well as in the oral cavity of humans (2). It is also the most common fungal pathogen in humans, causing both mucosal and systemic infections, particularly in immunocompromised individuals (3).…”

mentioning

confidence: 99%

Sustained Release of a Novel Anti-Quorum-Sensing Agent against Oral Fungal Biofilms

Feldman

Shenderovich

Al-Quntar

et al. 2015

Antimicrob Agents Chemother

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b Thiazolidinedione-8 (S-8) has recently been identified as a potential anti-quorum-sensing/antibiofilm agent against bacteria and fungi. Based on these results, we investigated the possibility of incorporating S-8 in a sustained-release membrane (SRM) to increase its pharmaceutical potential against Candida albicans biofilm. We demonstrated that SRM containing S-8 inhibits fungal biofilm formation in a time-dependent manner for 72 h, due to prolonged release of S-8. Moreover, the SRM effectively delivered the agent in its active form to locations outside the membrane reservoir. In addition, eradication of mature biofilm by the SRM containing S-8 was also significant. Of note, S-8-containing SRM affected the characteristics of mature C. albicans biofilm, such as thickness, exopolysaccharide (EPS) production, and morphogenesis of fungal cells. The concept of using an antibiofilm agent with no antifungal activity incorporated into a sustained-release delivery system is new in medicine and dentistry. This concept of an SRM containing a quorum-sensing quencher with an antibiofilm effect could pave the way for combating oral fungal infectious diseases.

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Opportunistic microorganisms in individuals with lesions of denture stomatitis

Cited by 81 publications

References 43 publications

Therapeutic Potential of Thiazolidinedione-8 as an Antibiofilm Agent against Candida albicans

Therapeutic Potential of Thiazolidinedione-8 as an Antibiofilm Agent against Candida albicans

Caspofungin‐induced in‐vitro post‐antifungal effect and its impact on adhesion related traits of oral Candida dubliniensis and Candida albicans isolates

Sustained Release of a Novel Anti-Quorum-Sensing Agent against Oral Fungal Biofilms

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