Oplopanax elatus adventitious root production through fed-batch culture and their anti-bacterial effects

Jin, Meizhong; Piao, Xiangfan; Wu, Xiaohan; Fan, Mingzhi; Li, X. F.; Yin, Chengri; Lian, Mei-Lan

doi:10.1007/s11240-019-01740-1

Cited by 17 publications

(19 citation statements)

References 27 publications

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“…Optimizing the culture conditions for O. elatus ARs in the bioreactor can largely increase its production ( Jiang et al, 2021 ). Extracts from cultured O. elatus Ars have demonstrated excellent anti-inflammatory, antioxidant, and antibacterial activities in our previous study ( Tian et al, 2019 ; Jin et al, 2020 ). However, the hepatoprotective effect of O. elatus ARs has not yet been investigated.…”

Section: Introductionmentioning

confidence: 75%

Hepatoprotective Effect of Oplopanax elatus Nakai Adventitious Roots Extract by Regulating CYP450 and PPAR Signaling Pathway

et al. 2022

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O. elatus Nakai is a traditional medicine that has been confirmed to exert effective antioxidant and anti-inflammatory functions, and is used for the treatment of different disorders. However, its potential beneficial effects on drug induced hepatotoxicity and relevant molecular mechanisms remain unclear. This study investigated the protective effect and further elucidated the mechanisms of action of O. elatus on liver protection. O. elatus chlorogenic acids-enriched fraction (OEB), which included chlorogenic acid and isochlorogenic acid A, were identified by HPLC-MS/MS. OEB was administrated orally daily for seven consecutive days, followed by a single intraperitoneal injection of an overdose of APAP after the final OEB administration. The effects of OEB on immune cells in mice liver were analyzed using flow cytometry. APAP metabolite content in serum was detected using HPLC-MS/MS in order to investigate whether OEB affects CYP450 activities. The intestinal content samples were processed for 16 s microbiota sequencing. Results demonstrated that OEB decreased alanine aminotransferase, aspartate aminotransferase contents, affected the metabolism of APAP, and decreased the concentrates of APAP, APAP-CYS and APAP-NAC by inhibiting CYP2E1 and CYP3A11 activity. Furthermore, OEB pretreatment regulated lipid metabolism by affecting the peroxisome proliferator-activated receptors (PPAR) signaling pathway in mice and also increased the abundance of Akkermansia and Parabacteroides. This study indicated that OEB is a potential drug candidate for treating hepatotoxicity because of its ability to affect drug metabolism and regulate lipid metabolism.

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Section: Introductionmentioning

confidence: 75%

Hepatoprotective Effect of Oplopanax elatus Nakai Adventitious Roots Extract by Regulating CYP450 and PPAR Signaling Pathway

et al. 2022

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“…Determination of electrical conductivity value, alkaline phosphatase activity, and nucleic acid and protein contents Electronic conductivity (EC) value, and alkaline phosphatase (AKP) activity, nucleic acid and protein contents were determined by using the method of Jin et al (2020). B. subtilis suspension (approximately 1⋅10 8 CFU/mL) was centrifuged for 4 min at 5,000 rpm, and the precipitated bacterial cells were collected.…”

Section: Bacterial Culturementioning

confidence: 99%

“…After 2 h of incubation (130 rpm) at 37°C, the mixture was centrifuged for 5 min at 5,000 rpm, and the supernatant was collected to determine AKP activity and nucleic acid and protein contents. Nucleic acid content was determined at an optical density (OD) of 260 nm (UV-2600, Shimadzu Corporation, Kyoto, Japan) according to Jin et al (2020). The AKP activity and protein content were determined by using an AKP assay kits (Nanjing JianCheng Bioengineering Institute, Nanjing, China) and Coomassie Brilliant Blue (CBB) (Nanjing JianCheng Bioengineering Institute), respectively, in accordance with the manufacturer's instructions.…”

Section: Bacterial Culturementioning

confidence: 99%

“…The intracellular protein content of B. subtilis was measured by applying the methods for bicinchoninic acid (BCA) assay and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis described by Jin et al (2020). In brief, 1 mL of B. subtilis suspension (approximately 1⋅10 8 CFU/mL) was inoculated into 100 mL of liquid BP medium and treatment with OE at 1/2MIC in the OE group.…”

Section: Bacterial Culturementioning

confidence: 99%

“…The inhibitory rate of respiration (I R ) against B. subtilis and superposition rate (S R ) was determined by measuring dissolved oxygen (DO) (Jin et al 2020). In brief, 2 mL of B. subtilis suspension (1⋅10 8 CFU/mL) was mixed with 10 mL of PBS (pH 7.4) and then, OE with 1/2MIC or 0.5 mg/mL typical respiratory inhibitors of iodoacetate acid (IA), malonate acid (MA), and sodium phosphate (SP), or a combination of both (i.e., OE + IA, OE + MA, and OE + SP).…”

Section: Determination Of the Inhibitory Rate Of Respirationmentioning

confidence: 99%

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Antibacterial mechanisms of Orostachys cartilaginous cell cultures: effect on cell permeability and respiratory metabolism of Bacillus subtilis

Liu

Jiang

et al. 2021

Plant Cell Tiss Organ Cult

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Orostachys cartilaginous of Crassulaceae family is a plant native to the Changbai Mountain area, China. Although O. cartilaginous has various medicinal values, its product development and production are restricted by the insu cient resource available. O. cartilaginous cell cultures possess an e cient antibacterial effect against Bacillis subtilis, but the underlying mechanism is not clear yet. Therefore, this study investigated the effects of extract from bioreactor cultured O. cartilaginous cells (OE) on B. subtilis cell permeability and respiratory metabolism to provide a reference for the further utilization of O. cartilaginous cell cultures. Results showed alkaline phosphatase activity, electrical conductivity, nucleic acid and protein contents in B. subtilis suspensions were signi cantly increased (p < 0.01) by OE treatment, indicating the occurrence of cell damage or increase in cell permeability. OE inhibited B. subtilis respiration, and the combination groups of OE+iodoacetic acid (IA) and OE+sodium phosphate (SP) showed low superposition rates (approximately 35%), revealing that OE likely affected IA-and SPrepresented metabolic pathways. The activities of B. subtilis enzymes, speci cally, hexokinase and pyruvate kinase in the Embden-Meyerhof (EMP) pathway and glucose-6-phosphate dehydrogenase in the pentose phosphate (HMP) pathway, decreased after OE treatment. This result proved that OE inhibited B. subtilis respiration by regulating the EMP and HMP pathways.

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Purification of Polysaccharides from Orostachys cartilaginous Cell Cultures by Macroporous Resin Absorption and Bioactivities of the Purified Polysaccharides

Han

et al. 2022

Chemistry & Biodiversity

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Orostachys cartilaginous is a traditional herbal medicine and its cell cultures contain large amounts of polysaccharides. To utilize the cultured O. cartilaginous cells, this study purified the crude polysaccharides of O. cartilaginous cells by macroporous resin absorption and optimized the purification process in the experiment of orthogonal design with four factors (sample concentration and volume, and eluent concentration and volume) and three levels; the antibacterial and anti‐cancer effects of the purified polysaccharides (OTP) were further examined. The results showed that polysaccharide purity reached 95 % in the optimized group, i. e., 1.6 mg/mL of sample (crude polysaccharides) concentration, 3.0 bed volume (BV) of sample volume, 65 % eluent (ethanol) concentration, and 3.0 BV of eluent volume. In the antibacterial experiment, the growth of three bacterial species, i. e., Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis was inhibited by OTP, whereas that of Escherichia coli was not affected; the largest diameter of the inhibitory zone was found on B. subtilis; the extracellular alkaline phosphatase activity and the electrical conductivity, nucleic acid, and protein levels of B. subtilis increased after OTP treatment, indicating that the inhibition of B. subtilis growth was caused by the leakage of cell contents. In the anti‐cancer experiment, OTP decreased the cell viabilities of the tested human cancer cells, i. e., AGS (gastric cancer), HCT116 (colon cancer), HepG2 (liver cancer), and HeLa (cervical cancer), and the highest inhibitory effect was on HCT116. OTP promoted HCT116 apoptosis and affected the expression of apoptosis‐related proteins, i. e., the expression of B‐cell lymphoma‐2 decreased and that of bcl‐2 associated X protein, cytochrome c, caspase 9 and caspase 3 increased. The findings of the present study suggest that O. cartilaginous cell cultures have a potential application in food or drug production.

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Oplopanax elatus adventitious root production through fed-batch culture and their anti-bacterial effects

Cited by 17 publications

References 27 publications

Hepatoprotective Effect of Oplopanax elatus Nakai Adventitious Roots Extract by Regulating CYP450 and PPAR Signaling Pathway

Hepatoprotective Effect of Oplopanax elatus Nakai Adventitious Roots Extract by Regulating CYP450 and PPAR Signaling Pathway

Antibacterial mechanisms of Orostachys cartilaginous cell cultures: effect on cell permeability and respiratory metabolism of Bacillus subtilis

Purification of Polysaccharides from Orostachys cartilaginous Cell Cultures by Macroporous Resin Absorption and Bioactivities of the Purified Polysaccharides

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