Opisthorchis viverrini Infection Augments the Severity of Nonalcoholic Fatty Liver Disease in High-Fat/High-Fructose Diet–Fed Hamsters

Abstract

The prevalence of nonalcoholic fatty liver disease (NAFLD) is increasing worldwide, including in regions where helminth infections such as the fish-borne liver fluke Opisthorchis viverrini (Ov) also occur. We investigated the effects of a high-fat and high-fructose (HFF) diet on the development and progression of NAFLD in experimental opisthorchiasis. Two groups of hamsters were infected with Ov for 4 months before the experiment to induce chronic inflammation. One of these groups (OvHFF) was fed with a HFF diet for up to further 4 months. One uninfected group of hamsters served as the normal control group, and another received the HFF diet (HFF group) for up to 4 months. Histopathology, biochemical parameters, and ultrastructural features of liver were investigated. In a short-term treatment, the OvHFF group showed significantly better homeostatic model assessment for insulin resistance level and lower liver lipid than did the HFF group. By contrast, histopathological characteristics of severe NAFLD were prominent in the OvHFF group after 4 months on the HFF diet, findings which were supported by confirmatory ultrastructural changes. In conclusion, opisthorchiasis induced the severe NAFLD in hamsters fed high-fat/high-fructose diets.

Curcumin-loaded nanocomplexes ameliorate the severity of nonalcoholic steatohepatitis in hamsters infected with Opisthorchis viverrini

“…Fifty live O . viverrini metacercariae were administered to each hamster by oral inoculation using gastric intubation [ 11 ].…”

“…The high-fat diet used was modified from that described previously [ 11 ]. The ingredients were a mixture of 40% control diet (Smart Heart; PCG, Bangkok, Thailand), 10% coconut oil (Roi Thai, Thailand), 10% corn oil (Golden Drop, Thailand), 1.25% cholesterol, 0.25% sodium deoxycholate (Sigma-Aldrich, St, Louis, Mo, USA), and 38.5% sucrose (Mitr Phol, Thailand).…”

“…Prior experimental study has demonstrated that O . viverrini infection and high-fat diets can rapidly accelerate the progression from NAFLD to NASH [ 11 ]. Although praziquantel is an effective drug for treating O .…”

A combination of monosodium glutamate and high-fat and high-fructose diets increases the risk of kidney injury, gut dysbiosis and host-microbial co-metabolism

“…The high-fat diet was prepared as previously described [28]. In brief, this diet was prepared by mixing 63.25% of the control diet (Smart Heart, Thailand) with 15% coconut oil, 15% corn oil, 0.5% cholesterol (Sigma-Aldrich, St. Louis, MO, USA), and 0.25% deoxycholate (Sigma-Aldrich), 6% tapioca starch (Fish Band, Thailand).…”

The Comparative Analysis of Blood Cell Morphology and Its Biochemical Features in Rabbits With Chronic Opisthorchiasis

“…Множество вовлеченных клеток разрушается при инвазии, как контактных с паразитом, так и удаленно расположенных. Паразиты группы описторхид (например, Opisthorchis viverrini) увеличивают концентрацию провоспалительных молекул, индуцируют повышенную экспрессию TLR-2, COX-2 и SOD-2 и пониженную экспрессию генов CAT у пациентов, дисбаланс оксидантов/антиоксидантов, изменяют углеводный и липидный обмен, даже при специальной диете [17][18][19].…”